The hydrolysis of cephalosporin G by free and immobilized penicillin G acylsse from a mutant of Escherichia coli ATCC 11105

Vandamme, 1990, Biochemical, genetical and biotechnical aspects of antibiotic production via immobilized biocatalysts, 379 Fujii, 1973, Enzymatic synthesis of cephalexin, Proc. Biochem., 11, 21 Toyo Jozo Company, 7-Aminodesacetoxycephalosporanic acid by enzymic deacylation of cephalosporins. Belgium Patent 801044, 1973. Vojtisek, 1989, Immobilized microbial cell aggregates for biotransformation of antibiotics, aminoacids, sugars and other compounds, Biotechnol. Appl. Biochem., 11, 1 Fleming, J. D., Turner, M. K. & Napier, E. J., Method of deacylating cephalosporins. British Patent 1473100, 1977. Erarslan, 1991, Fermentation of penicillin G acylase by a mutant strain of Escherichia coli ATCC 11105, Doğa — Tr. J. Biology, 15, 167 Erarslan, 1989, Purification and kinetic properties of penicillin acylase from Escherichia coli ATCC 11105, Doğa — Tr. J. Biology, 13, 8 Erarslan, 1990, Several immobilization methods of penicillin acylase purified from Escherichia coli ATCC 11105 and kinetic properties of enzyme immobilized on carboxymethyl-cellulose, Doğa — Tr. J. Eng. Env. Sci., 14, 381 Erarslan, 1991, Purification and kinetics of penicillin G acylase from a mutant strain of Escherichia coli ATCC 11105, J. Chem. Technol. Biotechnol., 51, 27, 10.1002/jctb.280510103 Erarslan, 1991, Kinetic investigation of penicillin G acylase from a mutant strain of Escherichia coli ATCC 11105 immobilized on oxirane-acrylic beads, J. Chem. Technol. Biotechnol., 51, 181, 10.1002/jctb.280510205 Meevootisom, 1983, Simple screening method for isolation of penicillin acylase producing bacteria, Appl. Environ. Microbiol., 46, 1227, 10.1128/AEM.46.5.1227-1229.1983 Krämer, D. (Röhm Pharma GmbH), Verfahren zur erstellung von stabilisierten trägergenundenen Proteinen. German Patent 2732301, 1979. Batchelor, 1961, 6-Aminopenicillanic acid. III. Isolation and purification, Proc. Roy. Soc., B, 154, 498, 10.1098/rspb.1961.0047 Shewale, 1987, Evaluation and determination of 6-aminopenicillanic acid by p-dimethylaminobenzaldehyde, Biotechnol. Techniques, 1, 69, 10.1007/BF00156291 Spector, 1978, Refinement of the Coomassie blue method of protein quantitation, Anal. Biochem., 86, 142, 10.1016/0003-2697(78)90327-5 Sedmak, 1977, A rapid, sensitive and versatile assay for protein using Coomassie brilliant blue G 250, Anal. Biochem., 79, 544, 10.1016/0003-2697(77)90428-6 Cole, 1969, Hydrolysis of penicillins and related compounds by the cell-bound penicillin acylase of Escherichia coli, Biochem. J., 115, 733, 10.1042/bj1150733 Margolin, 1980, Substrate specificity of penicillin amidase from E. coli., Biochim. Biophys. Acta, 616, 283, 10.1016/0005-2744(80)90145-X