Các yếu tố phiên mã lớp I thuộc họ homeodomain-leucine zipper (HD-Zip) ATHB7 và ATHB12 điều chỉnh tín hiệu axit abscisic bằng cách điều tiết hoạt động của gen protein phosphatase 2C và gen thụ thể axit abscisic

Plant Molecular Biology - Tập 80 - Trang 405-418 - 2012
Ana Elisa Valdés1,2, Elin Övernäs1, Henrik Johansson1, Alvaro Rada-Iglesias3, Peter Engström1,2
1Physiological Botany, Uppsala BioCenter, Uppsala University, Uppsala, Sweden
2Linnean Center for Plant Biology, Uppsala, Sweden
3Department of Chemical and Systems Biology, Stanford University, School of Medicine, Stanford, USA

Tóm tắt

Cây cảnh giác với tình trạng hạn hán kích hoạt nhiều phản ứng để cải thiện khả năng sống sót, bao gồm những thay đổi quy mô lớn trong biện pháp biểu hiện gen. Bài viết này báo cáo về vai trò trong phản ứng với tình trạng hạn hán của hai gen ATHB7 và ATHB12 thuộc lớp I homeodomain-leucine zipper của Arabidopsis thaliana; cả hai gen này đều có sự kích thích mạnh mẽ bởi sự thiếu nước và axit abscisic (ABA). Quá trình điều chỉnh phiên mã trung gian ABA của cả hai gen được chỉ ra là phụ thuộc vào hoạt động của protein phosphatase loại 2C (PP2C). Do đó, ATHB7 và ATHB12 là những mục tiêu của cơ chế tín hiệu ABA được xác định bởi PP2C và gia đình thụ thể ABA PYR/PYL, với protein PP2C tương tác. Kết quả của chúng tôi từ phân tích miễn dịch tinh thể chromatin và biểu hiện gen cho thấy ATHB7 và ATHB12 hoạt động như những điều tiết viên phiên mã dương tính của các gen PP2C, và do đó là những điều tiết viên âm tính của tín hiệu axit abscisic. Để hỗ trợ cho quan điểm này, kết quả của chúng tôi cũng cho thấy rằng ATHB7 và ATHB12 hoạt động để ức chế phiên mã của các gen mã hóa thụ thể ABA PYL5 và PYL8 trong phản ứng với kích thích ABA. Tóm lại, chúng tôi chứng minh rằng ATHB7 và ATHB12 có chức năng thiết yếu trong phản ứng ban đầu với hạn hán, như là những trung gian của tác động phản hồi âm tính trên tín hiệu ABA trong phản ứng của cây đối với tình trạng thiếu nước.

Từ khóa

#hạn hán #axit abscisic #ATHB7 #ATHB12 #protein phosphatase 2C #thụ thể axit abscisic

Tài liệu tham khảo

Ades SE, Sauer RT (1994) Differential DNA-binding specificity of the engrailed homeodomain: the role of residue 50. Biochem 33:9187–9194 Agalou A, Purwantomo S, Övernäs E, Johannesson H, Zhu X, Estiati A, de Kam RJ, Engström P, Slamet-Loedin IH, Zhu Z et al (2008) A genome-wide survey of HD-Zip genes in rice and analysis of drought-responsive family members. Plant Mol Biol 66:87–103 Barrero JM, Millar AA, Griffiths J, Czechowski T, Scheible WR, Udvardi M, Reid JB, Ross JJ, Jacobsen JV, Gubler F (2010) Gene expression profiling identifies two regulatory genes controlling dormancy and ABA sensitivity in Arabidopsis seeds. Plant J 61:611–622 Bowler C, Benvenuto G, Laflamme P, Molino D, Probst AV, Tariq M, Paszkowski J (2004) Chromatin techniques for plant cells. Plant J 39:776–789 Bray EA (2004) Genes commonly regulated by water-deficit stress in Arabidopsis thaliana. J Exp Bot 55:2331–2341 Czechowski T, Stitt M, Altmann T, Udvardi MK, Scheible W-R (2005) Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Plant Physiol 139:5–17 Deng X, Phillips J, Bräutigam A, Engström P, Johannesson H, Ouwerkerk PBF, Ruberti I, Salinas J, Vera P, Iannacone R et al (2006) A homeodomain leucine zipper gene from Craterostigma plantagineum regulates abscisic acid responsive gene expression and physiological responses. Plant Mol Biol 61:469–489 Dezar CA, Gago GM, González DH, Chan RL (2005) Hahb-4, a sunflower homeobox-leucine zipper gene, is a developmental regulator and confers drought tolerance to Arabidopsis thaliana plants. Transgenic Res 14:429–440 Fujii H, Zhu JK (2009) Arabidopsis mutant deficient in 3 abscisic acid-activated protein kinases reveals critical roles in growth, reproduction, and stress. PNAS 106:8380–8385 Fujii H, Chinnusamy V, Rodrigues A, Rubio S, Antoni R, Park S-Y, Cutler SR, Sheen J, Rodríguez PL, Zhu J-K (2009) In vitro reconstitution of an abscisic acid signalling pathway. Nature 462:660–664 Fujii H, Verslues PE, Zhu J-K (2011) Arabidopsis decuple mutant reveals the importance of SnRK2 kinases in osmotic stress responses in vivo. Proc Natl Acad Sci 108:1717–1722 González DH, Valle EM, Chan GG (1997) Interaction between proteins containing homeodomains associated to leucine zippers from sunflower. Biochem Biophys Acta 1351:137–149 Haring M, Offermann S, Danker T, Horst I, Peterhansel C, Stam M (2007) Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization. Plant Methods 3:11–26 Henriksson E, Olsson ASB, Johannesson H, Johansson H, Hanson J, Engström P, Söderman E (2005) Homeodomain leucine zipper class I genes in Arabidopsis. Expression patterns and phylogenetic relationships. Plant Physiol 139:509–518 Himmelbach A, Hoffmann T, Leube M, Höhener B, Grill E (2002) Homeodomain protein ATHB6 is a target of the protein phosphatase ABI1 and regulates hormone responses in Arabidopsis. EMBO J 21:3029–3038 Hjellström M, Olsson ASB, Engström P, Söderman E (2003) Constitutive expression of the water deficit-inducible homeobox gene ATHB7 in transgenic Arabidopsis causes a suppression of stem elongation growth. Plant Cell Env 26:1127–1136 Johannesson H, Wang Y, Engström P (2001) DNA-binding and dimerisation preferences of Arabidopsis homeodomain-leucine zipper transcription factors in vitro. Plant Mol Biol 45:63–73 Johannesson H, Wang Y, Hanson J, Engström P (2003) The Arabidopsis thaliana homeobox gene ATHB5 is a potential regulator of abscisic acid responsiveness in developing seedlings. Plant Mol Biol 51:719–729 Kobayashi Y, Yamamoto S, Minami H, Kagaya Y, Hattori T (2004) Differential activation of the rice sucrose nonfermenting 1-related protein kinase 2 family by hyperosmotic stress and abscisic acid. Plant Cell 16:1163–1177 Kuhn JM, Boisson-Dernier A, Dizon MB, Maktabi MH, Schroeder JI (2006) The protein phosphatase AtPP2CA negatively regulates abscisic acid signal transduction in Arabidopsis, and effects of abh1 on AtPP2CA mRNA. Plant Physiol 140:127–139 Lechner E, Leonhardt N, Eisler H, Alioua M, Jacquet H, Leung J, Genschik P (2011) MATH/BTB CRL3 receptors target the homeodomain-leucine zipper ATHB6 to modulate abscisic acid signalling. Dev Cell 21:1116–1128 Leung J, Giraudat J (1998) Abscisic acid signal transduction. Annu Rev Plant Physiol Plant Mol Biol 49:199–222 Ma S, Gong Q, Bohnert HJ (2007) An Arabidopsis gene network based on the graphical Gaussian model. Genome Res 17:1614–1625 Ma Y, Szostkiewicz I, Korte A, Moes D, Yang Y, Christmann A, Grill E (2009) Regulators of PP2C phosphatase activity function as abscisic acid sensors. Science 324:1064–1068 Merlot S, Gosti F, Guerrier D, Vavasseur A, Giraudat J (2001) The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway. Plant J 25:295–303 Miyazono K, Mikayawa T, Sawano Y, Kubota K, Kang H-J, Asano A, Miyauchi Y, Takahashi M, Zhi Y, Fujita K et al (2009) Structural basis of abscisic acid signalling. Nature 462:609–614 Murashige T, Skoog F (1962) A revised medium for growth and bioassays with tobacco tissue culture. Physiol Plant 15:493–497 Nakashima K, Fujita Y, Kanamori N, Katagiri T, Umezawa T, Kidokiro S, Maruyama K, Yoshida T, Ishiyama K, Kobayashi M et al (2009) Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, involved in ABA signaling are essential for the control of seed development and dormancy. Plant Cell Physiol 50:1345–1363 Olsson ASB (2004) HD-Zip I transcription factors in Arabidopsis thaliana: expression and function in relation to environmental stress conditions. Dissertation, Uppsala University Olsson ASB, Engström P, Söderman E (2004) The homeobox genes ATHB12 and ATHB7 encode potential regulators of growth in response to water deficit in Arabidopsis. Plant Mol Biol 55:663–677 Park SY, Fung P, Nishimura N, Jensen DR, Fujii H, Zhao Y, Lumba S, Santiago J, Rodrigues A, Chow TF et al (2009) Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science 324:1068–1071 Ré DA, Dezar CA, Chan RL, Baldwin IT, Bonaventure G (2011) Nicotiana attenuata NaHD20 plays a role in leaf ABA accumulation during water stress, bezylacetone emission from flowers and the timing of bolting and flower transitions. J Exp Bot 62:155–166 Reyes D, Rodríguez D, González-García MP, Lorenzo O, Nicolás G, García-Martínez JL, Nicolás C (2006) Overexpression of a protein phosphatase 2C from beech seeds in Arabidopsis shows phenotypes related to abscisic acid responses and gibberellin biosynthesis. Plant Physiol 141:1414–1424 Robert N, Merlot S, N′Guyen V, Boisson-Dernier A, Schroeder J (2006) A hypermorphic mutation in the protein phosphatases 2C HAB1 strongly affects ABA signalling in Arabidopsis. FEBS Lett 580:4691–4696 Rubio S, Rodrigues A, Saez A, Dizon MB, Galle A, Kim T-H, Santiago J, Flexas J, Schroeder JI, Rodríguez PL (2009) Triple loss of function of protein phosphatases type 2C leads to partial constitutive response to endogenous abscisic acid. Plant Physiol 150:1354–1355 Saez A, Apostolova N, González-Guzmán M, González-García MP, Nicolás C, Lorenzo O, Rodríguez PL (2004) Gain-of-function and loss-of-function phenotypes of the protein phosphatases 2C HAB1 reveal its role as a negative regulator of abscisic acid signaling. Plant J 37:354–369 Santiago J, Rodrigues A, Saez A, Rubio S, Antoni R, Dupeux F, Park S-Y, Márquez JA, Cutler SR, Rodríguez PL (2009) Modulation of drought resistance by the abscisic acid-receptor PYL5 through inhibition of clade A PP2Cs. Plant J 60:575–588 Schweighofer A, Hirt H, Meskiene I (2004) Plant PP2C phosphatases: emerging functions in stress signaling. Trends Plant Sci 9:236–243 Sessa G, Morelli G, Ruberti I (1993) The ATHB-1 and -2 HD-Zip domains homodimerize forming complexes of different DNA binding specificities. EMBO J 12:3507–3517 Shinozaki K, Yamaguchi-Shinozaki K, Seki M (2003) Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol 6:410–417 Söderman E, Mattsson J, Engström P (1996) The Arabidopsis homeobox gene ATHB-7 is induced by water deficit and by abscisic acid. Plant J 10:375–381 Son O, Hur Y-S, Kim Y-K, Lee H-J, Kim S, Kim M-R, Nam KH, Lee M-S, Kim B-Y, Park J et al (2010) ATHB12, an ABA-inducible homeodomain-leucine zipper (HD-Zip) protein of Arabidopsis, negatively regulates the growth of the inflorescence stem by decreasing the expression of a gibberellin 20-oxidase gene. Plant Cell Physiol 51:1537–1547 Umezawa T, Yoshida R, Maruyama K, Yamaguchi-Shinozaki K, Shinozaki K (2004) SRK2C, a SNF1-related protein kinase 2, improves drought tolerance by controlling stress-responsive gene expression in Arabidopsis thaliana. Proc Natl Acad Sci 101:17306–17311 Umezawa T, Sugiyama N, Mizoguchi M, Hayashi S, Myouga F, Yamaguchi-Shinozaki K, Ishihama Y, Hirayama T, Shinozaki K (2009) Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis. Proc Natl Acad Sci 106:17588–17593 Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3(7):research 0034.1–0034.11 Xiong L, Shumaker KS, Zhu J-K (2002) Cell signaling during cold, drought and salt stress. Plant Cell 14(Suppl.):S165–S183 Yoshida T, Nishimura M, Kitahata N, Kuromori T, Ito T, Asami T, Shinozaki K, Hirayama T (2006) ABA-Hypersensitive Germination3 encodes a protein phosphatase 2C (AtPP2CA) that strongly regulates abscisic acid signaling during germination among Arabidopsis protein phosphatase 2Cs. Plant Physiol 140:115–126 Zimmermann P, Hirsch-Hoffmann M, Hennig L, Gruissem W (2004) GENEINVESTIGATOR. Arabidopsis microarray database and analysis toolbox. Plant Physiol 136:2621–2632