Gen NF-YC tỏi, AsNF-YC8, điều chỉnh tích cực khả năng chịu áp lực thẩm thấu không ion ở thuốc lá

Protoplasma - Tập 254 - Trang 1353-1366 - 2016
Xiudong Sun1, Haifeng Lian1, Xingchen Liu1, Shumei Zhou2, Shiqi Liu1
1State Key Laboratory of Crop Biology, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region), College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, People’s Republic of China
2State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, People’s Republic of China

Tóm tắt

Để điều tra mối quan hệ giữa yếu tố nhân bản Y (NF-Y) và khả năng chịu stress trong tỏi, chúng tôi đã nhân bản một gen thuộc họ NF-Y, AsNF-YC8 từ tỏi, gen này được điều chỉnh tăng lên rõ rệt ở giai đoạn mất nước. Các phân tích mẫu biểu hiện trong tỏi cho thấy rằng AsNF-YC8 được kích thích thông qua axit abscisic (ABA) và các stress vô cơ, chẳng hạn như NaCl và PEG. So với các cây giống hoang dã, cây thuốc lá chuyển gen AsNF-YC8 biểu hiện quá mức cho thấy tỷ lệ nảy mầm hạt cao hơn, chiều dài rễ dài hơn và sự phát triển tốt hơn dưới các áp lực muối và hạn hán. Dưới áp lực hạn hán, các cây chuyển gen duy trì hàm lượng nước tương đối (RWC) cao hơn, quá trình quang hợp ròng, mức độ malondialdehyde (MDA) thấp hơn, và ít rò rỉ ion (IL) hơn so với các cây giống hoang dã đối chứng. Những kết quả này cho thấy khả năng chịu đựng cao của các cây chuyển gen trước áp lực hạn hán so với dòng WT. Các dòng thuốc lá chuyển gen tích lũy ít loài oxy phản ứng (ROS) hơn và biểu hiện hoạt tính enzyme chống oxy hóa cao hơn so với các cây giống hoang dã (WT) dưới áp lực hạn hán, điều này cho thấy rằng sự biểu hiện quá mức của AsNF-YC8 cải thiện hệ thống phòng thủ chống oxy hóa thông qua việc điều chỉnh hoạt tính của các enzyme chống oxy hóa này, từ đó bảo vệ các dòng chuyển gen khỏi áp lực hạn hán. Những kết quả này gợi ý rằng AsNF-YC8 đóng một vai trò quan trọng trong khả năng chịu đựng stress do hạn hán và muối.

Từ khóa

#yếu tố nhân bản Y #NF-Y #tỏi #khả năng chịu stress #áp lực thẩm thấu #thuốc lá #axit abscisic #ion #oxy phản ứng #enzyme chống oxy hóa

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