Tỷ lệ tổng hợp phân đoạn của protein huyết tương được xác định bằng cách sử dụng nước deuterated nhạy cảm với lượng lysine trong chế độ ăn của chuột

Amino Acids - Tập 50 - Trang 1719-1727 - 2018
Ying Tian1, Minghui Shi1, Qianqian Dai1, Chanfang Meng1, Ruixia Gu2,3, Jing Peng1, Yu Chen1, Yunsheng Jiang1
1Department of Nutrition, School of Food Science and Engineering, Yangzhou University, Yangzhou, China
2Department of Food Science and Engineering, School of Food Science and Engineering, Yangzhou University, Yangzhou, China
3Jiangsu Provincial Key Laboratory of Dairy Biotechnology and Safety Control, School of Food Science and Engineering, Yangzhou University, Yangzhou, China

Tóm tắt

Truyền thống, tác động của lysine từ chế độ ăn đối với sức khỏe được xác định thông qua nồng độ của protein trong huyết tương, nhưng đôi khi chúng không phản ứng với lượng lysine tiêu thụ. Chúng tôi giả thuyết rằng tỷ lệ tổng hợp phân đoạn (FSR) của protein huyết tương có thể nhạy cảm hơn với chế độ ăn lysine hơn là nồng độ protein trong huyết tương. Bảy mươi hai con chuột Sprague-Dawley đực được chia ngẫu nhiên thành ba nhóm dựa trên chế độ ăn trong 18 tuần: lysine thấp (LG), lysine bình thường (NG) và lysine cao (HG). Chuột được gán nhãn bằng nước deuterated, một loại chất đánh dấu đáng tin cậy hơn so với các chất đánh dấu amino acid. FSR của albumin và immunoglobulin (Ig) G trong huyết tương tăng lên khi lượng lysine trong chế độ ăn tăng. Tuy nhiên, nồng độ albumin trong huyết tương ở chuột thuộc nhóm LG không giảm đáng kể so với nhóm NG, và một kết quả tương tự cũng được thấy ở nồng độ IgG giữa nhóm NG và HG. Những kết quả này cho thấy rằng FSR của albumin và IgG trong huyết tương nhạy cảm hơn với lượng lysine trong chế độ ăn so với nồng độ của chúng, và có thể hữu ích như những chỉ số nhạy cảm về tác động của lysine trong chế độ ăn đối với sức khỏe.

Từ khóa

#lysine #protein huyết tương #chuột Sprague-Dawley #tỷ lệ tổng hợp phân đoạn #nước deuterated

Tài liệu tham khảo

Belloto E, Fdr Diraison, Basset A, Allain G, Abdallah P, Beylot M (2007) Determination of protein replacement rates by deuterated water: validation of underlying assumptions. Am J Physiol Endocrinol Metab 292:1340–1347 Bonjour J (2005) Dietary protein: an essential nutrient for bone health. J Am Coll Nutr 24:526–536 Busch R et al (2006) Measurement of protein turnover rates by heavy water labeling of nonessential amino acids. Biochem Biophys Acta 1760:730–744 Canfield LM, Chytil F (1978) Effect of low lysine diet on rat protein metabolism. J Nutr 108:1343–1347 Chen Y-Y, Lin S-Y, Yeh Y-Y, Hsiao H-H, Wu C-Y, Chen S-T, Wang AH-J (2005) A modified protein precipitation procedure for efficient removal of albumin from serum. Electrophoresis 26:2117–2127 Debro J, Korner A (1956) Solubility of albumin in alcohol after precipitation by trichloroacetic acid: a simplified procedure for separation of albumin. Nature 178:1067 Dufner DA et al (2005) Using 2H2O to study the influence of feeding on protein synthesis: effect of isotope equilibration in vivo vs. in cell culture. Am J Physiol Endocrinol Metab 288:1277–1283 Gasier HG, Riechman SE, Wiggs MP, Previs SF, Fluckey JD (2009) A comparison of 2H2O and phenylalanine flooding dose to investigate muscle protein synthesis with acute exercise in rats. Am J Physiol Endocrinol Metab 297:252–259 Gasier HG, Fluckey JD, Previs SF (2010) The application of 2H2O to measure skeletal muscle protein synthesis. Nutr Metab 7:31–38 Ghosh S, Pellett PL, Aw-Hassan A, Mouneime Y, Smriga M, Scrimshaw NS (2008) Impact of lysine-fortified wheat flour on morbidity and immunologic variables among members of rural families in northwest Syria. Food Nutr Bull 29:163–171 Gietzen D, Rogers Q (2006) Nutritional homeostasis and indispensable amino acid sensing: a new solution to an old puzzle. Trends Neurosci 29:91–99 Goto S, Nagao K, Bannai M, Takahashi M, Nakahara K, Kangawa K, Murakami N (2010) Anorexia in rats caused by a valine-deficient diet is not ameliorated by systemic ghrelin treatment. Neuroscience 166:333–340 Hao S et al (2005) Uncharged tRNA and sensing of amino acid deficiency in mammalian piriform cortex. Science 307:1776–1778 Holm L et al (2013) Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans. Am J Physiol Endocrinol Metab 304:895–907 Hrupka BJ, Lin Y, Gietzen DW, Rogers QR (1999) Lysine deficiency alters diet selection without depressing food intake in rats. J Nutr 129:424–430 Huang L et al (2011) Lysine requirement of the enterally fed term infant in the first month of life. Am J Clin Nutr 94:1496–1503 Jackson AA, Phillips G, Mcclelland I, Jahoor F (2001) Synthesis of hepatic secretory proteins in normal adults consuming a diet marginally adequate in protein. Am J Physiol Gastrointest Liver Physiol 281:1179–1187 James WP, Hay AM (1968) Albumin metabolism: effect of the nutritional state and the dietary protein intake. J Clin Invest 47:1958–1972 Jeffay H, Winzler RJ (1958) The metabolism of serum proteins. II. The effect of dietary protein on the turnover of rat serum protein. J Biol Chem 231:111–116 Khan L, Bamji M (1979) Tissue carnitine deficiency due to dietary lysine deficiency: triglyceride accumulation and concomitant impairment in fatty acid oxidation. J Nutr 109:24–31 Li P, Yin YL, Li D, Kim SW, Wu G (2007) Amino acids and immune function. Br J Nutr 98:237–252 Nagao K, Bannai M, Seki S, Kawai N, Mori M, Takahashi M (2010) Voluntary wheel running is beneficial to the amino acid profile of lysine-deficient rats. Am J Physiol Endocrinol Metab 298:1170–1178 Nielsen K, Nansen P (1967) Metabolism of bovine immunoglobulin II. Metabolism of bovine igg in cattle with secondary hypoimmunoglobulinemia. Can J Comp Med Vet Sci 31:106–110 Petro TM, Bhattacharjee JK (1980) Effect of dietary essential amino acid limitations upon native levels of murine serum immunoglobulins, transferrin, and complement. Infect Immun 27:513–518 Pillai RR, Elango R, Ball RO, Kurpad AV, Pencharz PB (2015) Lysine requirements of moderately undernourished school-aged indian children are reduced by treatment for intestinal parasites as measured by the indicator amino acid oxidation technique. J Nutr 145:954–959 Previs SF, Fatica R, Chandramouli V, Alexander JC, Brunengraber H, Landau BR (2004) Quantifying rates of protein synthesis in humans by use of 2H2O: application to patients with end-stage renal disease. Am J Physiol Endocrinol Metab 286:665–672 Reeves PG, Nielsen FH, Fahey GC (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951 Regmi N, Wang T, Crenshaw M, Rude BJ, Liao SF (2017) Effects of dietary lysine levels on the concentrations of selected nutrient metabolites in blood plasma of late-stage finishing pigs. J Anim Physiol Anim Nutr 102:1–12 Schwert GW (1957) Recovery of native bovine serum albumin after precipitation with trichloroacetic acid and solution in organic solvents. J Am Chem Soc 79:139–141 Shimomura A et al (2014) Dietary l-lysine prevents arterial calcification in adenine-induced uremic rats. J Am Soc Nephrol 25:1954–1965 Smriga M, Kameishi M, Uneyama H, Torii K (2002) Dietary l-lysine deficiency increases stress-induced anxiety and fecal excretion in rats. J Nutr 132:3744–3746 Volpi E et al (1996) Contribution of amino acids and insulin to protein anabolism during meal absorption. Diabetes 45:1245–1252 Wada Y, Sato Y, Miyazakia K, Takedaa Y, Kuwahatac M (2017) The reduced/oxidized state of plasma albumin is modulated by dietary protein intake partly via albumin synthesis rate in rats. Nutr Res 37:46–57 Wei Z, Peebles E, Wang X, Gerard P, Olanrewaju H, Mercier Y (2016) Effects of dietary lysine and methionine supplementation on Ross 708 male broilers from 21 to 42 d of age (III): serum metabolites, hormones, and their relationship with growth performance. J Appl Poultry Res 25:223–231 Wen Z, Rasolofomanana T, Tang J, Jiang Y, Xie M, Yang P, Hou S (2017) Effects of dietary energy and lysine levels on growth performance and carcass yields of Pekin ducks from hatch to 21 days of age. Poult Sci 96:3361–3366 Wilson W, Roach P (2002) Nutrient-regulated protein kinases in budding yeast. Cell 111:155–158 Zhao W et al (2004) Lysine-fortified wheat flour improves the nutritional and immunological status of wheat-eating families in northern China. Food Nutr Bull 25:123–129