The fluctuation of APC gene in WNT signaling with adenine deletion of adenomatous polyposis coli, is associated in colorectal cancer

Journal of Coloproctology - Tập 40 - Trang 135-142 - 2020
Seyedeh Elham Norollahi1, Seyed Mohammad Taghi Hamidian2, Zeynab Khazaee Kohpar3, Rezvan Azadi4, Pooya Rostami5, Sogand Vahidi1, Sahar Ghazanfari6, Farnaz Azar Shabe3, Roya Khaksar7, Ali Akbar Samadani1
1Guilan University of Medical Sciences, Gastrointestinal and Liver Diseases Research Center, Rasht, Iran
2Babol University of Medical Sciences, Department of Gastroenterology, Babol, Iran
3Islamic Azad University of Tonekabon Branch, Department of Biology, Tonekabon, Iran
4Shahid Beheshti University of Medical Sciences, Department of Medicine, Tehran, Iran
5New York University, Londgone Medical Center, Brooklyn, NY, USA
6Tarbiyat Modares University, Department of Mycology, Tehran, Iran
7Islamic Azad University of Tehran Shargh, Department of Cellular and Molecular Biology, Tehran, Iran

Tóm tắt

AbstractColorectal cancer is one of the most important malignancies in the classification of gastrointestinal cancers. One of the predisposing factors at molecular level for this cancer is via WNT signaling which is associated with the vast numbers of different genes. Thus, in this study, we aimed to investigate whether Adenomatous Polyposis Coli gene (APC) mutation of rs41115in two locations such as 132.002 and 131.989 acts as a trigger or cause of colorectal cancer. Relatively, 30 blood samples of colorectal cancer patients and 30 normal blood samples as control group after colonoscopy and also confirmation of pathology report at Rohani Hospital in Babol (Iran) were investigated. The primers were designed in order to be included the rs41115 to identify the particular polymorphisms of gene. The polymerase chain reaction (PCR direct sequencing method) was used. Conclusively, deletion of adenine in two specific locations such as 131.989 and 132.002 has been identified, but there was no relationship between rs41115 polymorphisms located in adenomatous polyposis coli gene and colorectal cancer.

Tài liệu tham khảo

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