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Sự biểu hiện của microRNA-34a có mối tương quan nghịch với c-MET và CDK6 và có ý nghĩa tiên lượng ở bệnh nhân ung thư phổi biểu mô tuyến
Tóm tắt
Chúng tôi nhằm mục đích xác định xem sự biểu hiện của microRNA-34a (miR-34a) có tương quan với c-MET và các yếu tố điều hòa giai đoạn G1 như cyclin dependent kinase (CDK) 4, CDK6, và cyclin D (CCND) 1 trong ung thư phổi không nhỏ (NSCLC), và liệu có mối liên hệ nào giữa sự biểu hiện của miR-34a và các yếu tố lâm sàng bệnh lý cũng như thời gian sống không tái phát (RFS). Đối với 58 mẫu được lưu trữ từ bệnh nhân NSCLC, chúng tôi đã đo lường sự biểu hiện của miR-34a và c-MET, CDK4/6, và CCND1 bằng phương pháp RT-PCR định lượng và đánh giá mối quan hệ giữa sự biểu hiện của miR-34a, các yếu tố lâm sàng-bệnh lý và RFS. Sự biểu hiện của miR-34a thấp hơn đáng kể trong các khối u tế bào vảy (P < 0.001) và trong các khối u liên quan đến sự xâm lấn bạch huyết (P = 0.001). Chúng tôi nhận thấy có mối tương quan nghịch đáng kể giữa miR-34a và c-MET (R = −0.316, P = 0.028) và biểu hiện CDK6 (R = −0.4582, P = 0.004). Thời gian sống không tái phát dài hơn ở bệnh nhân adenocarcinoma có sự biểu hiện miR-34a cao hơn so với những người có sự biểu hiện miR-34a thấp (55.6 so với 21.6 tháng; P = 0.020). Với phân tích đơn biến, các yếu tố tiên lượng có ý nghĩa thống kê cho RFS ở bệnh nhân adenocarcinoma bao gồm sự biểu hiện miR-34a (Nguy cơ tương đối (RR), 8.14; P = 0.049), giai đoạn TNM (RR, 13.55; P = 0.001), di căn hạch bạch huyết (RR, 4.19; P = 0.043), và sự hiện diện của xâm lấn bạch huyết (RR, 7.05; P = 0.015). Trong phân tích đa biến, chỉ có miR-34a có ý nghĩa tiên lượng cho RFS (RR, 11.5; P = 0.027). Sự biểu hiện miR-34a có tương quan nghịch với c-MET và CDK6 trong NSCLC, và có ý nghĩa tiên lượng cho RFS, đặc biệt là ở bệnh nhân adenocarcinoma.
Từ khóa
#microRNA-34a #c-MET #CDK6 #ung thư phổi không nhỏ #prognostic significance #sống không tái phátTài liệu tham khảo
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