The effects of carbonate and Mg on in vitro mineralization of demineralized dentin
Tóm tắt
The demineralized sections of dentins of rats were mineralized in vitro. The sections were observed with contact microradiography (CMR) and their mineral contents were determined with micro-photometry of CMR films. In the mineralizing solution containing no carbonate, mineral appeared to adhere only on the surface of the demineralized dentins, regardless of their structure or their chemical nature. However, once the demineralized dentins were immersed in a mineralizing solution containing carbonate, the mineralization proceeded continuounly even in mineralizing solution containing no carbonate, suggesting that the action of carbonate participates in the initiation of mineral deposition. The effect of Mg in the mineralizing solution containing carbonate was studied. Mg showed an inhibitory effect in the mineralization of matrices. This effect seemed to participate in the process of growth or increase of precipitation after the initiation.
Tài liệu tham khảo
Shipley, P.G., Kramer, B. and Howland, J.: Studies upon calcification in vitro. Biochem. J. 20, 379–387, 1926.
Robison, R., Macleod, M. and Rosenheim, A.H.: The possible significance of hexosephosphoric esters in ossification. IX. Calcification in vitro. Biochem. J. 24, 1927–1941, 1930.
Sobel, A.E. and Hanok, A.: Calcification. VII. Reversible inactivation of calcification in vitro and related studies. J. Biol. Chem. 197, 669–685, 1952.
Masuhara, T. and Okada, M.: Physiology and pharmacology of hard tissues (report 52). Calcification in vitro (3). Folia Pharmacol. Japon. 53, 174, 1957.
Bachra, B.N., Sobel, A.E. and Stanford, J.W.: Calcification. XXIV. Mineralization of collagen and other fibers. Archs. Biochem. Biophys. 84, 79–95, 1959.
Solomons, C.C. and Neuman, W.F.: On the mechanisms of calcification: The remineralization of dentin. J. Biol. Chem. 235, 2502–2506, 1960.
Thomas, W.C. and Tomita, A.: Mineralization of human and bovine tissue in vitro. Am. J. Pathol. 51, 621–628, 1967.
Bachra, B.N. and Ischer, H.R.A.: Mineral deposition in collagen in vitro. Calcif. Tissue Res. 2, 343–352, 1968.
Katz, E.P.: The kinetics of mineralization in vitro. I. The nucleation properties of 640 A collagen at 25. Biochim. Biophys. Acta 194, 121–129, 1969.
Jethi, R.K. and Wadkins, C.L.: Studies of the mechanism of biological calcification. II. Evidence for a multi-step mechanism of calcification by tendon matrix. Calcif. Tissue Res. 7,277–289, 1971.
Bachra, B.N.: Calcification in vitro of demineralized bone Matrix. Electron microscopic and chemical aspects. Calcif. Tissue Res. 8,287–303, 1972.
Luben, R.A., Sherman, J.K. and Wadkins, C.L.: Studies of the mechanism of biological calcification. IV. Ultrastructural analysis of calcifying tendon matrix. Calcif. Tissue Res. 11,39–55, 1973.
DeSteno, C.V. and Feagin, F.F.: Effect of matrix bound phosphate and fluoride on mineralization of dentin. Calcif. Tissue Res. 17,151–159, 1975.
DeSteno, C.V., Feagin, F.F. and Butler, W.T.: Mineralization of dentin, bone and tendon in vitro. Calcif. Tissue Res. 17,161–163, 1975.
Boyer, D.B. and Eirich, F.R.: Remineralization of dentin in vitro. Calcif. Tissue Res. 21, 17–28, 1976.
Quittner, C. and Wadkins, C.L.: A macromolecular inhibitor of in vitro calcification of tendon matrix. Calcif. Tissue Res. 25, 161–168, 1978.
Wadkins, C.L.: In vitro mineralization of bovine aorta. In: The chemistry and biology of mineralized connective tissues. (Veis, A.ed.) Elsevier/North-Holland, pp. 273–277, 1981.
Driessens, F.C.M.: Mineral aspects of dentistry. Karger, 1982.
Bachra, B.N., Trautz, O.R. and Simon, S.L.: Precipitation of calcium carbonates and phosphates. I. Spontaneous precipitation of calcium carbonates and phosphates under physiological conditions. Archs. Biochem. Biophys. 103, 124–138, 1963.
Greenwald, I.: The effect of phosphate on the solubility of calcium carbonate and of bicarbonate on the solubility of calcium and magnesium phosphates. J.Biol. Chem. 161, 697–704, 1945.
Kuyper, A.C.: The chemistry of bone formation. II. Some factors which affect the solubility of calcium phosphate in blood serum. J. Biol. Chem. 159, 417–424, 1945.
Bachra, B.N., Trautz, O.R. and Simon, S.L.: Precipitation of calcium carbonates and phosphates. III. The effect of magnesium and fluoride ions on the spontaneous precipitation of calcium carbonate and phosphates. Archs. oral Biol. 10,731–738, 1965.
Takeishi, S.: Physiology and Pharmacology of hard tissues (report 35). Studies on the antagonistic action between magsesium and calcium (5). Folia Pharmacol. Japon. 49,180, 1953.
Ogata, T: Comparison of dentin formed in vivo and dentin mineralized in vitro by contact microradiography: Effect of Mg in mineralizing solution. J.B.M.M. 6, 1988, in press.