The effect of selenium on the localization of autometallographic mercury in dorsal root ganglia of rats
Tóm tắt
The autometallographic technique was used to demonstrate the localization of mercury in dorsal root ganglia of adult Wistar rats. The animals were either exposed to mercury vapour, 100 μg Hg m−3, 6 h day−1, 5 days per week, or treated with organic mercury in the drinking water, 20 mg CH3HgCl per litre, for 4 weeks. The effect of orally administered sodium selenite on the pattern of intracellular distribution of mercury in these two situations was investigated. In rats exposed to mercury vapour alone, faint staining was present in ganglion cells. The selenite induced a conspicuous increase in the number of stained cells and in the intracellular staining intensity. In rats treated with organic mercury, mercury deposits were detected within ganglion cells and macrophages. The number of mercury-containing cells was increased by co- administration of selenite. In addition, satellite cells, the capsule and vessel walls were faintly stained. Twenty weeks after cessation of the organic mercury treatment, mercury staining was reduced. Again, selenite treatment enhanced staining intensity. When studied using the electron microscope, mercury was restricted to lysosomes, irrespective of treatments. The present study shows that the deposition of autometallographic mercury in the dorsal root ganglia depends on the chemical type of mercury, the co-administration of selenite and the length of the survival period.
Tài liệu tham khảo
Albers, J.W., Cavender, G., Levine, S.P. & Langolf, G.D. (1982) Asymptomatic sensorimotor polyneuropathy in workers exposed to elementary mercury. Neurology32, 1168–74.
Albers, J.W., Kallenbach, L.R., Lawrence, J.F., Langolf, G.D., Wolfe, R.A., Donofrio, P.D., Alessi, A.G., Stolp-Smith, K.A., Bromberg, M.B., AND The Mercury Workers Study Group (1988) Neurological abnormalities associated with remote occupational elemental mercury exposure. Ann. Neurol.25, 651–9.
Alexander, J. & Norseth, T. (1979) The effect of selenium on the biliary excretion and organ distribution of mercury in the rat after exposure to methyl mercuric chloride. Acta Pharmacol. Toxicol.44, 168–76.
Aschner, M. & Aschner, J.L. (1990) Mercury neurotoxicity: mechanisms of blood-brain barrier transport. Neurosci. Biobehav. Rev.14, 169–76.
Baatrup, E. & Danscher, G. (1987) Cytochemical demonstration of mercury deposits in trout liver and kidney following methyl mercury intoxication: differentiation of two mercury pools by selenium. Ecotoxicol. Environ. Saf.14, 129–41.
Baatrup, E., Nielsen, G.N. & Danscher, G. (1986) Histochemical demonstration of two mercury pools in trout tissues: mercury in kidney and liver after mercuric chloride exposure. Ecotoxicol. Environ. Saf.12, 267–82.
Baatrup, E., Thorlacius-Ussing, O., Nielsen, H.L. & Wilsky, K. (1989) Mercury-selenium interactions in relation to histochemical staining of mercury in the rat liver. Histochem. J.21, 89–98.
Berlin, M. (1986) Mercury. In Handbook of the Toxicology of Metals, 2nd edn. (edited by Friberg, L., Nordberg, G.F. & Vouk, V.B.), pp. 346–87. Amsterdam, New York: Elsevier/North Holland.
Carmichael, N., Cavanagh, J.B. & Rodda, R.A. (1975) Some effects of methyl mercury salts on the rabbit nervous system. Acta Neuropathol.32, 115–25.
Chang, L.W. (1983) Protective effects of selenium against methylmercury neurotoxicity: a morphological and biochemical study. Exp. Pathol.23, 143–56.
Chapman, L.J., Sauter, S.L., Henning, R.A., Dodson, V.N., Reddan, W.G. & Matthews, C.G. (1990) Differences in frequency of finger tremor in otherwise asymptomatic mercury workers. Br. J. Indust. Med.47, 838–43.
Clarkson, T.W., Hursh, J.B., Sager, P.R. & Syversen, T. (1988) Mercury. In Biological Monitoring of Toxic Metals (edited by Clarkson, T.W., Friberg, L., Nordberg, G.F. & Sager, P.R.), pp. 199–246. New York: Plenum Press.
Danscher, G. & MØller-Madsen, B. (1985) Silver amplification of mercury sulphide and selenide: a histochemical method for light and electron microscopic localization of mercury in tissue. J. Histochem. Cytochem.33, 219–28.
Eide, R. & Wesenberg, G.B.R. (1990) A simple system for generating low-dosage mercury vapor for animals experiments. Acta Odontol. Scand.48, 251–6.
Fukuda, K. (1971) Metallic mercury induced tremor in rabbits and mercury content of the central nervous system. Br. J. Indust. Med.28, 308–11.
Garman, R.H., Weiss, B. & Evans, H.L. (1975) Alkylmercurial encephalopathy in the monkey (Saimiri sciurius and Macaca arctoides). Acta Neuropathol.32, 61–74.
Halbach, S. & Clarkson, T.W. (1978) Enzymatic oxidation of mercury vapor by erythrocytes. Biochim. Biophys. Acta523, 522–31.
Hansen, J.C., Kristensen, P. & Westergaard, I. (1981) The influence of selenium on mercury distribution in mice after exposure to low dose Hg· vapours. J. Appl. Toxicol.3, 149–53.
Hargreaves, R.J., Evans, J.G., Janota, I., Magos, L. & Cavanagh, J.B. (1988) Persistent mercury in the nerve cells 16 years after metallic mercury poisoning. Neuropathol. Appl. Neurobiol.14, 443–52.
Iwata, H., Okamoto, H. & Oshawa, Y. (1973) Effect of selenium on methyl mercury poisoning. Res. Commun. Chem. Pathol. Pharmacol.5, 673–80.
Jacobs, J.M. (1982) Vascular permeability and neurotoxicity. In Nervous System Toxicology (edited by Mittshell, C.L.), pp. 285–98. New York: Raven Press.
Jacobs, J.M., Carmichael, N. & Cavanagh, J.B. (1975a) Ultrastructural changes in the dorsal root and trigeminal ganglia of rats poisoned with methyl mercury. Neuropathol. Appl. Neurobiol.1, 1–19.
Jacobs, J.M., Cavanagh, J.B. & Carmichael, N. (1975b) The effect of chronic dosing with mercuric chloride on dorsal root and trigeminal ganglia of rats. Neuropathol. Appl. Neurobiol.3, 321–37.
Jacobs, J.M., Carmichael, N. & Cavanagh, J.B. (1977) Ultrastructural changes in the nervous system of rabbits poisoned with methyl mercury. Toxicol. Appl. Pharmacol.39, 249–61.
Kishi, R., Hashimoto, K., Shimizu, S. & Kobayashi, M. (1978) Behavioral changes and mercury concentrations in tissues of rats exposed to mercury vapor. Toxicol. Appl. Pharmacol.46, 555–66.
Liebermann, A.R. (1976) Sensory ganglia. In The Peripheral Nerve (edited by Landon, D.N.), pp 188–278. London: Chapman & Hall.
Magos, L. (1968) Uptake of mercury by the brain. Br. J. Indust. Med.25, 315–8.
Magos, J. & Weeb, M. (1977) The effect of selenium on the brain uptake of methylmercury. Arch. Toxicol.38, 201–7.
Magos, L. & Weeb, M. (1980) The interactions of selenium with cadmium and mercury. Crit. Rev. Toxicol.8, 1–42.
Magos, L., Halbach, S. & Clarkson, T.W. (1978) Role of catalase in the oxidation of mercury vapor. Biochem. Pharmacol.27, 1373–7.
Miyama, T., Minowa, K., Seki, H., Tanura, Y., Mizoguchi, I., Ohi, G. & Suzuki, T. (1983) Chronological relationship between neurological signs and electrophysiological changes in rats with methylmercury poisoning — special reference to selenium protection. Arch. Toxicol.52, 173–81.
MØller-Madsen, B. & Danscher, G. (1991) Localization of mercury in CNS of the rat. IV. The effect of selenium on orally administered organic and inorganic mercury. Toxicol. Appl. Pharmacol.108, 457–73.
Nielsen-Kudsk, F. (1969) Uptake of mercury vapour in blood in vivo and in vitro from Hg-containing air. Acta Pharmacol. Toxicol.27, 149–60.
NØrgaard, J.O.R., MØller-Madsen, B. & Danscher, G. (1991) Autometallographic localization of mercury in rat kidney — interaction between mercury and selenium. Prog. Histochem. Cytochem.23, 187–93.
Nygaard, S.P. & Hansen, J.C. (1978) Mercury-selenium interaction at concentrations of selenium and of mercury vapours as prevalent in nature. Bull. Environ. Contam. Toxicol.20, 20–3.
Ohi, G., Nishigaki, S., Seki, H., Tamura, Y., Maki, T., Maeda, H., Ochiai, S., Yamada, H., Shimamura, Y. & Yagyu, H. (1975) Interaction of dietary methylmercury and selenium on accumulation and retention of these substances in rat organs. Toxicol. Appl. Pharmacol.32, 527–33.
Ohi, G., Nishigaki, S., Seki, H., Tamura, Y., Maki, T., Konno, H., Ochiai, S., Yamada, H., Shimamura, Y., Mizoguchi, I. & Yagyu, H. (1976) Efficacy of selenium in tuna and selenite in modifying methylmercury intoxication. Environ. Res.12, 49–58.
Ohi, G., Nishigaki, S., Seki, H., Tamura, Y., Maki, T., Minowa, K., Shimamura, Y. & Mizoguchi, I. (1980) The protective potency of marine animal meat against the neurotoxicity of methylmercury: its relationship with the organ distribution of mercury and selenium in the rat. Food Cosmet. Toxicol.18, 139–45.
SchiØnning, J. & MØller-Madsen, B. (1991) Autometallographic mapping of mercury deposits in the spinal cord of rats treated with inorganic mercury. Acta Neuropathol.81, 434–42.
SchiØnning, J.D., MØller-Madsen, & Danscher, G.S (1991) Mercury in dorsal root ganglia of rats treated with inorganic or organic mercury. Environ. Res.56, 48–56.
SchiØnning, J.D., Eide, R., MØller-Madsen, B. & Ernst, E. (1993) Detection of mercury in rat spinal cord and dorsal root ganglia after exposure to mercury vapor. Exp. Mol. Pathol.58, 215–28.
Singer, R., Valciukas, J.A. & Rosenman, K.D. (1987) Peripheral neurotoxicity in worker exposed to inorganic mercury compounds. Arch. Environ. Health42, 181–4.
Stillings, B.R., Lagally, H., Bauersfield, P. & Soares, J. (1974) Effect of cystine, selenium and fish protein on the toxicity and metabolism of methylmercury in rats. Toxicol. Appl. Pharmacol.30, 243–54.
Takeuchi, T., Morikawa, N., Matsumoto, H. & Shiraishi, Y. (1962) A pathological study of Minamata disease in Japan. Acta Neuropathol.2, 40–57.
Thomas, D.J. & Smith, J.C. (1984) Effects of co-administered sodium selenite on short-term distribution of methyl mercury in the rat. Environ. Res.34, 287–94.
Whittaker, S.G., Smith, D.G. & Foster, J.R. (1991) Cytochemical localization of mercury in Saccharomyces cerevisiae treated with mercury chloride. J. Histochem. Cytochem.38, 823–7.
Yip, R.K. & Chang, L.W. (1981) Vulnerability of dorsal root neurons and fibers toward methylmercury toxicity: a morphological evaluation. Environ. Res.26, 152–67.