The effect of cycloheximide on IAA-stimulated transport of 14C-ABA and 14C-sucrose in long pea epicotyl segments

The effect of cycloheximide (CH) on the indol-3yl-acetic acid (IAA)-stimulated transport of 14C-labelled abscisic acid (ABA) and 14C-labelled sucrose was studied in 110 mm long pea epicotyl segments. IAA application resulted in elongation growth of the segments. This effect was decreased by CH treatment which also reduced [14C] ABA and [14C] sucrose accumulation in the growing apical part of the segments. A reduction in [14C] IAA uptake and in protein synthesis in this part of the segments was also observed. The simultaneous inhibition of protein synthesis and reduction of [14C] ABA and [14C] sucrose transport suggests that IAA can stimulate the transport of ABA and sucrose through a protein synthesis-based elongation growth.