The effect of active components of Lycium barbarum and garlic (LB-GO) on the synthesis of dna and ultrastructure of u14 cervix cancer cells in mice

Mice, inoculated with U14 ascitic type cervix cancer cells, were administered with effective components of Lyclum barbarum (LB) and garlic (GO). On the 4th day afteri.p. administration, general condition of the animals were found improved. Examination of ascitic fluid revealed damage of the cancer cells, blanching of fluorescence staining of DNA and RNA, and the cancer cells besieged by large numbers of macrophages and leucocytes. Flow cell metric (FCM) analysis found: accumulation of cells of G1 stage. Ultrastructure study disclosed: swelling of mitochondria in cytoplasm and damage of mitochondrial crests even with cavity formation; enlargement and degranulation of rough ER. It seemed the effect of LBGO was affirmable. It was postulated that macrophages, being activated by LB, came in close contact with the cancer cells giving rise to carcinolysls. In addition to the direct but transient killing effect of GO, the anti-cancer results could be greatly enhanced.