The direct measurement of inhibitory capacity to crystal growth of calcium oxalate in undiluted urine and in other inhibitor containing solutions

Urological Research - Tập 8 - Trang 171-175 - 1980
J. M. Baumann1,2, M. Wacker1,2
1Department of Urology, Regionalspital, Biel, Switzerland
2Stone Research Laboratory, Regionalspital, Biel, Switzerland

Tóm tắt

This paper describes a simple method to measure the capacity of undiluted urine and of other inhibitor containing solutions (PPi and EHDP) to protect a given mass of calcium oxalate crystals from growth. The method has also been used to determine relative urinary saturation with respect to calcium oxalate. It is based on titration with oxalate and measures the critical changes of concentration necessary to reach saturation or to induce crystal growth. From these changes inhibitory capacity as well as the level of urinary saturation can be calculated in terms of differences of concentration products. The use of the methods at present available to measure urinary oxalate are thereby avoided. In order to compare the results from different urines without the need for cumbersome calculations of activity products we have introduced a saturation-inhibition ratio.

Tài liệu tham khảo

Baumann, J. M., Bisaz, S., Felix, R., Fleisch, H., Ganz, U., Russel, R. G. G.: The role of inhibitors and other factors in the pathogenesis of recurrent calcium-containing renal stones. Clinical Science and Molecular Medecine 53, 141 (1977) Baumann, J. M., Wacker, M.: Experiences with the Measurement of Inhibitory Activity of Urine and Crystallisation Inhibitors by Different Techniques. Urological Research 7, 183 (1979) Finlayson, B., Reid, F.: The Expectation of free and fixed Particles in Urinary Stone Disease. Investigative Urology 15, 442 (1978) Fleisch, H.: Inhibitors and promoters of stone formation. Kidney International 13, 361 (1978) Meyer, J. L., Smith, L. H.: Growth of calcium oxalate crystals. I. A model for urinary stone growth. Investigative Urology 13, 31 (1975) Pak, C. Y. C., Hayashi, Y., Finlayson, B., Chu, S.: Estimation of the state of saturation of brushite and calcium oxalate in urine: A comparison of three methods. Journal of Laboratory and Clinical Medicine 89, 891 (1977) Robertson, W. G., Peacock, M., Nordin, B. E. C.: Activity products in stone-forming and non-stone-forming urine. Clinical Science and Molecular Medecine 34, 579 (1968) Robertson, W. G., Peacock, M., Marshall, R. W., Marshall, D. H., Nordin, B. E. C.: Saturation-inhibition index as a measure of the risk of calcium oxalate stone formation in the urinary tract. New England Journal of Medecine 294, 249 (1976)