The comet assay: a comprehensive review

Ager, 1990, Calibration of pulsed-field gel electrophoresis for measurement of DNA double-strand breaks, Int. J Radiat. Biol., 58, 249, 10.1080/09553009014551601 Ahnström, 1988, Techniques to measure single-strand breaks in cells: A review, Int. J Radiat. Biol., 54, 695, 10.1080/09553008814552151 Ahnström, 1974, Radiation-induced single-strand breaks in DNA determined by rate of alkaline strand separation and hydroxyapatite chromatography: an alternative to velocity sedimentation, Int. J. Radiat. Biol., 26, 493, 10.1080/09553007414551511 Ahuja, 1993, Single cell gel electrophoresis on leukocytes of patients with precancerous and cancerous lesions of the cervix, Am. J Hum. Gen., 53, 1515 Alderson, 1993, Fas transduces activation signals in normal human T-lymphocytes, J Exp. Med., 178, 2231, 10.1084/jem.178.6.2231 Ames, 1983, Dietary carcinogens and anticarcinogens, Science, 221, 1256, 10.1126/science.6351251 Anderson, 1994, The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay, Mutation Res., 307, 261, 10.1016/0027-5107(94)90300-X Arends, 1990, Apoptosis: the role of the endonuclease, Am. J Pathol., 136, 593 Arlett, 1993, Hypersensitivity of human lymphocytes to UV-B and solar irradiation, Cancer Res., 53, 609 Barber, 1993, The role of oxygenation in embryotoxic mechanisms of three bioreducible agents, Teratology, 47, 209, 10.1002/tera.1420470305 Barnard, 1994 Betti, 1993, Comparative studies on cytotoxic and genotoxic effects of two organic mercury compounds in lymphocytes and gastric mucosa cells of Sprague-Dawley rats, Env. Mol. Mutagen., 22, 172, 10.1002/em.2850220310 Betti, 1994, Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects, Mutation Res., 307, 232, 10.1016/0027-5107(94)90306-9 Bose, 1992, Effect of linker length on DNA-binding affinity, cross-linking efficiency and cytotoxicity of C8-linked pyrrolobenzodiazepine dimers, J Chem. Soc. Chem. Commun., 20, 1518, 10.1039/c39920001518 Bradley, 1979, X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution, Nucleic Acids Res., 7, 793, 10.1093/nar/7.3.793 Brown, 1993, SR4233 (Tirapazamine): a new anticancer drug exploiting hypoxia in solid tumours, Br. J. Cancer, 67, 1163, 10.1038/bjc.1993.220 Cerutti, 1985, Prooxidant states and tumor promotion, Science, 227, 375, 10.1126/science.2981433 Cheeseman, 1993, An introduction to free radical biochemistry, Br. Med. Bull., 49, 481, 10.1093/oxfordjournals.bmb.a072625 Collins, 1992, 7, 357 Collins, 1993, Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA, Carcinogenesis, 14, 1733, 10.1093/carcin/14.9.1733 Combes, 1992, Genotoxicity testing: recent advances and future trends, Chem. Indust., 24, 950 Cook, 1975, Supercoils in human DNA, J Cell Science, 19, 261, 10.1242/jcs.19.2.261 Correa, 1992, Human gastric carcinogenesis: a multi-step and multi-factorial process — First American Cancer Society award lecture on cancer epidemiology and prevention, Cancer Res., 52, 6735 Deeley, 1992, Heterogeneity of radiation-induced damage measured by microgel electrophoresis, Int. J Radiat. Biol., 62, 372 Delaney, 1993, DNA damage in rat islet cells and HIT-T15 cells exposed to interleukin-1β and nitric oxide- studies using the comet assay, Diabetologia, 36, A77 Delaney, 1993, Endogenous nitric oxide induced by interleukin-1β in rat islets of Langerhans and HIT-T15 cells causes significant DNA damage as measured by the ‘comet’ assay, FEBS lett., 333, 291, 10.1016/0014-5793(93)80673-I De Méo, 1991, Genotoxic activity of potassium permanganate in acidic solutions, Mutation Res., 260, 295, 10.1016/0165-1218(91)90038-N Dewith, 1994, UV-B-laser-induced DNA-damage in lymphocytes observed by single-cell gel-electrophoresis, J Photochem. Photobiol. B. Biology, 24, 47, 10.1016/1011-1344(94)07007-5 Dive, 1992, Induction of apoptosis — new targets for cancer chemotherapy, Sem. Cancer Biol., 3, 417 Duvall, 1985, Macrophage recognition of cells undergoing programmed cell death (apoptosis), Immunology, 56, 351 Evans, 1993, DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines, Radiat. Res., 134, 307, 10.2307/3578190 Fairbairn, 1994, Detection of apoptosis induced DNA cleavage in scrapie infected sheep brain, FEMS Microbiol. Lett., 115, 341, 10.1111/j.1574-6968.1994.tb06661.x Fairbairn, 1994, Detection of DNA damaging agents in environmental water samples, Bull. Environ. Contam. Tox., 52, 687, 10.1007/BF00195488 Fairbairn, 1994, The effect of electromagnetic field exposure on the formation of DNA single strand breaks in human cells, Cell. Molec. Biol., 40, 561 Fairbairn, 1993, Application of confocal laser scanning microscopy to analysis of H2O2-induced DNA damage in human cells, Scanning, 15, 136, 10.1002/sca.4950150305 Fairbairn, 1994, Laser scanning microscopic analysis of DNA damage in frozen tissues, Cancer Lett., 76, 127, 10.1016/0304-3835(94)90388-3 Fairbairn, 1994, Alkali-labile sites are prevalent in kidney tissue DNA, Cancer Lett., 81, 67, 10.1016/0304-3835(94)90166-X Fearon, 1990, A genetic model for colorectal tumorigenesis, Cell, 61, 759, 10.1016/0092-8674(90)90186-I Fertil, 1984, Detection in situ of Á-ray induced DNA strand breaks in single cells: enzymatic labeling of free 3′-Oh ends, Int. J. Radiat. Biol., 46, 529, 10.1080/09553008414551741 Felsenfeld, 1992, Chromatin as an essential part of the transcriptional mechanism, Nature, 355, 219, 10.1038/355219a0 Floyd, 1994, Rapid measurement of DNA damage at low doses of radiation: experience with the comet assay, Int. J Radiat. Biol., 65, 126 Freeman, 1986, Quantitation of radiation-, chemical-, or enzyme-induced single-strand breaks in nonradioactive DNA by alkaline gel electrophoresis: application to pyrimidine dimers, Anal. Biochem., 158, 119, 10.1016/0003-2697(86)90599-3 Frenkel, 1992, Carcinogen-mediated oxidant formation and oxidative DNA damage, Pharmacol. Ther., 53, 127, 10.1016/0163-7258(92)90047-4 Futscher, 1992, Gene specific DNA interstrand crosslinks produced by nitrogen mustard in the human tumor line COLO320HSR, Cell Growth Diff., 3, 217 Gedik, 1992, Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells, Int. J Radiat. Biol., 62, 313, 10.1080/09553009214552161 Green, 1994, Effects of cytokines and nitric oxide donors on insulin secretion, cyclic GMP and DNA damage: relation to nitric oxide production, Biochem. Soc. Trans., 22, 30, 10.1042/bst0220030 Green, 1992, UV-C sensitivity of unstimulated and stimulated human lymphocytes from normal and xeroderma pigmentosum donors in the comet assay: A potential diagnostic technique, Mutation Res., 273, 137, 10.1016/0921-8777(92)90075-E Green, 1994, Effect of diet and vitamin C on DNA strand breakage in freshly-isolated human white blood cells, Mutation Res., 316, 91, 10.1016/0921-8734(94)90011-6 Green, 1994, Effect of deoxyribonucleosides on the hypersensitivity of human peripheral blood lymphocytes to UV-B and UV-C irradiation, Mutation Res., 315, 25, 10.1016/0921-8777(94)90024-8 Grigsby, 1993, Differential DNA damage detected in hybridomas, Hybridoma, 12, 755, 10.1089/hyb.1993.12.755 Guyton, 1993, Oxidative mechanisms in carcinogenesis, Br. Med. Bull., 49, 523, 10.1093/oxfordjournals.bmb.a072628 Hara, 1994, A single-cell gel-electrophoresis technique for the detection of DNA-damage induced by ACNU, an alkylating agent or irradiation in murine glioma cell lines, Neurol. Res., 16, 234, 10.1080/01616412.1994.11740234 Harland, 1983, Transcription of DNA injected into Xenopus oocytes is influenced by template topology, Nature, 302, 38, 10.1038/302038a0 Hartley, 1993, Electrophoretic and chromatographic separation methods used to reveal interstrand crosslinking of nucleic acids, J Chromatogr., 618, 277, 10.1016/0378-4347(93)80038-6 Hartmann, 1994, Does physical activity induce DNA damage?, Mutagenesis, 9, 269, 10.1093/mutage/9.3.269 Hartmann, 1994, Comparative investigations of the genotoxic effects of metals in the single-cell gel (SCG) assay and the sister-chromatid exchange (SCE) test, Env. Mol. Mutagen., 23, 299, 10.1002/em.2850230407 Hickman, 1992, Apoptosis induced by anticancer drugs, Cancer Metast. Rev., 11, 121, 10.1007/BF00048059 Higami, 1994, An age-related increase in the basal level of DNA damage and DNA vulnerability to oxygen radicals in the individual hepatocytes of male F344 rats, Mutation Res., 316, 59, 10.1016/0921-8734(94)90008-6 Hirai, 1993, Evaluation of chemically induced DNA damage in mouse hepatocyte culture using the single cell gel (SCG) electrophoretic assay, Mutation Res., 292, 268, 10.1016/0165-1161(93)90048-5 Iliakis, 1991, The role of DNA double-strand breaks in ionizing radiation-induced killing of eukaryotic cells, BioEssays, 13, 641, 10.1002/bies.950131204 Imlay, 1988, Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro, Science, 240, 640, 10.1126/science.2834821 Imlay, 1988, DNA damage and oxygen radical toxicity, Science, 240, 1302, 10.1126/science.3287616 Jostes, 1993, Single-cell gel technique supports hit probability calculations, Health Physics, 64, 675, 10.1097/00004032-199306000-00016 Kane, 1993, Bcl-2 inhibition of neural death: decreased generation of reactive oxygen species, Science, 262, 1274, 10.1126/science.8235659 Kennelly, 1993, Genotoxic activity of 1-chloromethylpyrene in stomach epithelium in vivo: insensitivity of the stomach scintillation UDS assay, Carcinogenesis, 14, 637, 10.1093/carcin/14.4.637 Kent, 1994, Optimization of image endpoints in the comet assay, Int. J Rad. Biol., 65, 125 Kleiman, 1993, DNA single strand breaks in human lens epithelial cells from patients with cataract, Current Eye Res., 12, 423, 10.3109/02713689309024624 Kohn, 1981, Measurement of strand breaks and crosslinking by alkaline elution, Vol 1, 379 Kovacs, 1990, Investigation of the repair of single strand breaks in human DNA using alkaline gel electrophoresis, Mutation Res., 124, 137 Kreja, 1993, In Vitro study on the radiosensitivity of canine hemopoietic and stroma cells with the single cell gel (‘comet’) assay, Exp. Hematol., 21, 1051 Kruszewski, 1994, DNA strand breakage, cytotoxicity and mutagenicity of hydrogen peroxide treatment at 4C and 37C in L5178Y sublines, Mutation Res., 308, 233, 10.1016/0027-5107(94)90158-9 Laget, 1993, Mutagenicity of 16 imidazo [1,2-a] pyridine by the Ames test and the Comet assay, Mutation Res., 291, 252 Latinwo, 1994, Effects of low levels of ionizing particles on rat kidney DNA, FASEB J, 8, A906 Lehman, 1970, Double-strand breaks in the DNA of a mammalian cell after X-irradiation, Biochim. Biophys. Acta, 217, 268, 10.1016/0005-2787(70)90526-5 Lewis, 1986, The effect of macrophage development on the release of reactive oxygen intermediates and lipid oxidation products, and their ability to induce oxidative DNA damage in mammalian cells, Carcinogenesis, 7, 813, 10.1093/carcin/7.5.813 Martins, 1991, Role of antioxidants in protecting cellular DNA from damage by oxidative stress, Mutation Res., 250, 95, 10.1016/0027-5107(91)90166-L McGrath, 1966, Reconstruction in vivo of irradiated E. coli deoxyribonucleic acid; the rejoining of broken pieces, Nature, 212, 534, 10.1038/212534a0 McKelvey, 1992, Analysis of DNA content and integrity in cells extracted from bladder washing and voided urine specimens, in bladder cancer patients, using the comet assay, Mutation Res., 271, 163, 10.1016/0165-1161(92)91198-Z McKelvey, 1991, Decreased DNA repair capacity in APRT-deficient Friend erythroleukaemia cells following H2O2 treatment, Mutagenesis, 6, 441 McKelvey, 1992, Induction of DNA damage by low level laser irradiation in Friend mouse erythroleukaemia cells, Mutation Res., 271, 131, 10.1016/0165-1161(92)91121-7 McKelvey, 1990, A technique for measuring DNA single strand breaks and alkali labile sites in individual cells, Br. J Cancer, 62, 529 McKelvey-Martin, 1993, The single cell gel electrophoresis assay (comet assay): A European review, Mutation Res., 288, 47, 10.1016/0027-5107(93)90207-V McMillan, 1990, Measurement of radiation-induced DNA damage, Int. J Radiat. Biol., 58, 391, 10.1080/09553009014551751 Meyers, 1993, Measuring the repair of H2O2-induced DNA single strand breaks using the single cell gel assay, Cytobios, 74, 147 Michalek, 1993, Application of the single cell microgel electrophoresis to detect genotoxic effects in rat pancreatic cells, Mutation Res., 291, 256 Moraes, 1990, Mutagenesis by hydrogen peroxide treatment of mammalian cells: a molecular analysis, Carcinogenesis, 11, 283, 10.1093/carcin/11.2.283 Moraes, 1989, The spectrum of mutations generated by passage of a hydrogen peroxide damaged shuttle vector plasmid through a mammalian host, Nucleic Acids Res., 17, 8301, 10.1093/nar/17.20.8301 Morris, 1984, Hormone-induced cell death. Surface changes in thymocytes undergoing apoptosis, Am. J. Pathol., 115, 426 Müller, 1994, Comet assay studies of radiation-induced DNA damage and repair in various tumour cell lines, Int. J Radiat. Biol., 65, 315, 10.1080/09553009414550371 Nordén, 1991, Microscopic behavior of DNA: electrophoretic orientation, Q. Rev. Biophys., 24, 103, 10.1017/S0033583500003395 Nose, 1983, Detection of carcinogen-induced by DNA breaks by nick translation in permeable cells, Biochem. Biophys. Res. Commun., 111, 383, 10.1016/0006-291X(83)90317-0 Olive, 1989, Cell proliferation as a requirement for development of the contact effect in Chinese hamster V79 spheroids, Radiat. Res., 117, 79, 10.2307/3577279 Olive, 1992, DNA organization affects cellular radiosensitivity and detection of initial DNA strand breaks, Int. J Radiat. Biol., 62, 389, 10.1080/09553009214552261 Olive, 1994, Radiation-induced reoxygenation in the SCCVII murine tumor: evidence for a decrease in oxygen consumption and an increase in tumor perfusion, Radiother. Oncol., 10.1016/0167-8140(94)90447-2 Olive, 1993, Detection of DNA double-strand breaks through the cell cycle after exposure to X-rays, bleomycin, etoposide and 125IdUrd, Int. J Radiat. Biol., 64, 349, 10.1080/09553009314551531 Olive, 1992, Growth fraction measured using the comet assay, Cell Prolif., 25, 447, 10.1111/j.1365-2184.1992.tb01453.x Olive, 1993, Induction and rejoining of radiation-induced DNA single-strand breaks: ‘tail moment’ as a function of position in the cell cycle, Mutation Res., 294, 275, 10.1016/0921-8777(93)90010-E Olive, 1990, Detection of etoposide resistance by measuring DNA damage in individual Chinese hamster cells, J. Natl. Cancer Inst., 82, 779, 10.1093/jnci/82.9.779 Olive, 1990, Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the ‘comet’ assay, Radiat. Res., 122, 86, 10.2307/3577587 Olive, 1993, Cell killing and DNA damage by etoposide in Chinese hamster V79 monolayers and spheroids: influence of growth kinetics, growth environment and DNA packaging, Br. J. Cancer, 67, 522, 10.1038/bjc.1993.97 Olive, 1994, Influence of DNA strand breakage and DNA structure on staining by propidium iodide using the comet assay, Cytometry, 10.1002/cyto.990160404 Olive, 1988, Comparison between the DNA precipitation and alkali unwinding assays for detecting DNA strand breaks and cross-links, Cancer Res., 48, 6444 Olive, 1992, Detection of hypoxic cells in a murine tumor with the use of the comet assay, J Natl. Cancer Inst., 84, 707, 10.1093/jnci/84.9.707 Olive, 1991, Etoposide sensitivity and topoisomerase II activity in Chinese hamster V79 monolayers and small spheroids, Int. J Radiat. Biol., 60, 453, 10.1080/09553009114552311 Olive, 1993, Gel electrophoresis of individual cells to quantify hypoxic fraction in human breast cancers, Cancer Res., 53, 733 Olive, 1993, Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay, Radiat. Res., 136, 130, 10.2307/3578650 Olive, 1993, Predicting tumor response to radiotherapy using the comet assay, 65 Olive, 1993, Growth fraction of human tumors: assays and complications, Vol 3, 90 Olive, 1994, Lack of correlation between DNA double-strand break induction/rejoining and radiosensitivity in six human tumor cell lines, Cancer Res. Olive, 1994, Hypoxic fractions measured in murine tumors and normal tissues using the comet assay, Inter. J. Radiat. Oncol. Biol. Phys., 29, 487, 10.1016/0360-3016(94)90443-X Olive, 1991, DNA double-strand breaks measured in individual cells subjected to gel eleetrophoresis, Cancer Res., 51, 4671 Olive, 1992, Factors influencing DNA migration from individual cells subjected to gel electrophoresis, Exp. Cell Res., 198, 259, 10.1016/0014-4827(92)90378-L O'Neill, 1993, Analysis of single cell gel electrophoresis using laser-scanning microscopy, Mutation Res., 319, 129, 10.1016/0165-1218(93)90071-K Östling, 1987, Bleomycin, in contrast to gamma irradiation, induces extreme variation of DNA strand breakage from cell to cell, Int. J Radiat. Biol., 52, 683, 10.1080/09553008714552201 Östling, 1984, Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells, Biochem. Biophys. Res. Commun., 123, 291, 10.1016/0006-291X(84)90411-X Östling, 1987, DNA damage in clinical radiation therapy studied by microelectrophoresis in single tumor cells, Acta Oncol., 26, 45, 10.3109/02841868709092977 Paranjape, 1994, Role of chromatin structure in the regulation of transcription by RNA polymerase II, Annu. Rev. Biochem., 63, 265, 10.1146/annurev.bi.63.070194.001405 Plappert, 1994, Early effects of benzene exposure in mice. Hematological versus genotoxic effects, Arch. Toxicol., 68, 284, 10.1007/s002040050070 Plappert, 1993, Rapid detection of benzene toxicity in mice using the comet assay, Exp. Hematol., 21, 1042 Pool-Zobel, 1993, Antigenotoxic properties of lactic acid bacteria in vivo in the gastrointestinal tract of rats, Nutr. Cancer, 20, 271, 10.1080/01635589309514295 Pool-Zobel, 1993, Assessment of genotoxic effects by lindane, Food Chem. Tox., 31, 271, 10.1016/0278-6915(93)90077-C Pool-Zobel, 1992, Systemic genotoxic effects of tobacco-related nitrosamines following oral and inhalation administration to Sprague-Dawley rats, Clin. Invest., 70, 299, 10.1007/BF00184666 Pool-Zobel, 1992, Detection of genotoxicity in cells of the gastrointestinal tract, Mutagenesis, 7, 166 Pool-Zobel, 1994, Detection of genotoxic effects in human gastric and nasal-mucosa cells isolated from biopsy samples, Env. Mol. Mutagen., 24, 23, 10.1002/em.2850240105 Roti Roti, 1987, Visualization of DNA loops in nucleoids from HeLa cells: assays for DNA damage and repair, Cytometry, 8, 461, 10.1002/cyto.990080505 Rowe, 1994, Formaldehyde induces DNA single strand breaks at very low concentrations, Cytobios Rydberg, 1984, Detection of DNA damage in single cells using flow cytometry, Int. J. Rad. Biol., 46, 521, 10.1080/09553008414551731 Rydberg, 1975, The rate of strand separation in alkali of DNA of irradiated mammalian cells, Radiat. Res., 61, 274, 10.2307/3574045 Rydberg, 1978, Estimation of DNA strand breaks in single mammalian cells, 465 Schmezer, 1994, Tissue-specific induction of mutations by streptozotocin in vivo, Mutation Res., 307, 495, 10.1016/0027-5107(94)90260-7 Schwartz, 1984, Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis, Cell, 37, 67, 10.1016/0092-8674(84)90301-5 Shafer, 1994, Detection of opiate-enhanced increases in DNA damage, HPRT mutants and the mutation frequency in human HUT-78 cells, Env. Mol. Mutagen., 23, 37, 10.1002/em.2850230107 Simmonds, 1992, Chemilluminescence assay of mucosal reactive oxygen metabolites in inflammatory bowel disease, Gastroenterology, 103, 186, 10.1016/0016-5085(92)91112-H Singh, 1990, DNA damage and repair with age in individual human lymphocytes, Mutation Res., 237, 123, 10.1016/0921-8734(90)90018-M Singh, 1989, Abundant alkali-sensitive sites in DNA of human and mouse sperm, Exp. Cell Res., 184, 461, 10.1016/0014-4827(89)90344-3 Singh, 1991, Basal DNA damage in individual human lymphocytes with age, Mutation Res., 256, 1, 10.1016/0921-8734(91)90026-8 Singh, 1988, A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res., 175, 184, 10.1016/0014-4827(88)90265-0 Singh, 1994, Modifications of alkaline microgel electrophoresis for sensitive detection of DNA damage, Int. J. Radiat. Biol., 66, 23, 10.1080/09553009414550911 Singh, 1991, A microgel technique for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides, Mutation Res., 252, 289, 10.1016/0165-1161(91)90008-V Smith, 1992, Simultaneous measurement of cell cycle phase position and ionizing radiation-induced DNA strand breakage in single human tumour cells using laser scanning confocal imaging, Int. J Rad. Biol., 61, 553, 10.1080/09553009214551331 Stratford, 1986, The differential cytotoxicity of RSU 1069: Cell survival studies indicating interaction with DNA as a possible mode of action, Br. J Cancer, 53, 339, 10.1038/bjc.1986.57 Strauss, 1994, Measuring DNA damage in individual cells of heterogeneous mixtures: a novel application of an immunological typing technique, Mutation Res., 304, 211, 10.1016/0027-5107(94)90213-5 Sun, 1992, Characterization of apoptosis in thymocytes isolated from dexamethasone-treated rats, Biochem. Pharmacol., 44, 2131, 10.1016/0006-2952(92)90339-K Taj, 1994, Induction of genotoxicity by salted deep-fried fish and mutton, Mutation Res., 322, 45, 10.1016/0165-1218(94)90031-0 Tice, 1991, The single cell gel (SCG) assay: an electrophoretic technique for the detection of DNA damage in individual cells, Adv. Exp. Med. Biol., 283, 157, 10.1007/978-1-4684-5877-0_17 Tice, 1990, The single cell gel assay: a sensitive technique for evaluating intercellular differences in DNA damage and repair, Basic Life Sci., 53, 291 Tice, 1992, High-dose combination alkylating agents with autologous bone-marrow support in patients with breast cancer: preliminary assessment of DNA damage in individual peripheral blood lymphocytes using the single cell gel electrophoresis assay, Mutation Res., 271, 101, 10.1016/0165-1161(92)91083-4 Uzawa, 1994, Radiosensitivity of CD45RO + memory and CD45RO− naive T cells in culture, Radiat. Res., 137, 25, 10.2307/3578787 Vaux, 1993, Toward an understanding of the molecular mechanisms of physiological cell death, 90, 786 ijayalaxmi, 1993, An analysis of gamma-ray-induced DNA damage in human blood leukocytes, lymphocytes and granulocytes, Mutation Res., 292, 123, 10.1016/0165-1161(93)90139-Q Vijayalaxmi, 1992, Assessment of radiation-induced DNA damage in human blood lymphocytes using the single-cell gel electrophoresis technique, Mutation Res., 271, 243, 10.1016/0165-1161(92)90019-I Vogelstein, 1980, Supercoil loops and eukaryotic DNA replication, Cell, 22, 79, 10.1016/0092-8674(80)90156-7 Vollmar, 1992, Expression of atrial natriuretic peptide in thymic macrophages after dexamethasone treatment of rats, Cell Tissue Res., 268, 397, 10.1007/BF00318809 Ward, 1993, A sensitivity to oxidative stress is linked to chromosome-11 but is not due to a difference in single-strand DNA breakage or repair, Mutation Res., 294, 299, 10.1016/0921-8777(93)90012-6 Ward, 1994, Response of fibroblast cultures from ataxiatelangiectasia patients to reactive oxygen species generated during inflammatory reactions, Environ. Mol. Mutagen., 10.1002/em.2850240205 Whitaker, 1991, Molecular assays of radiation-induced DNA damage, Eur. J Cancer, 27, 922, 10.1016/0277-5379(91)90148-7 Williams, 1991, Programmed cell death: apoptosis and oncogenesis, Cell, 65, 1097, 10.1016/0092-8674(91)90002-G Wyllie, 1992, Apoptosis and the regulation of cell numbers in normal and neoplastic tissues: An overview, Cancer Metast. Rev., 11, 95, 10.1007/BF00048057 Zhang, 1993, Radiosensitization and inhibition of deoxyribonucleic acid repair in rat glioma cells by long term treatment with 12-O-tetradecanoylphorbol 13-acetate, Neurosurgery, 32, 432, 10.1227/00006123-199303000-00015