The TomatoAux/IAATranscription FactorIAA9Is Involved in Fruit Development and Leaf Morphogenesis

Plant Cell - Tập 17 Số 10 - Trang 2676-2692 - 2005
Hua Wang1, Brian Jones1, Zhengguo Li2, Pierre Frasse1, Corinne Delalande1, Farid Regad1, Salma Chaabouni1, Alain Lachaux1, Jean‐Claude Pech1, Mondher Bouzayen1
1 Unité Mixte de Recherche 990  Institut National de la Recherche Agronomique/Institut National Polytechnique–Ecole Nationale Supérieure Agronomique Toulouse  Génomique et Biotechnologie des Fruits Pôle de Biotechnologie Végétale  31326 Castanet-Tolosan Cedex  France
2 Genetic Engineering Research Center  Chongqing University  40030 Chongqing  China

Tóm tắt

AbstractAuxin/indole-3-acetic acid (Aux/IAA) proteins are transcriptional regulators that mediate many aspects of plant responses to auxin. While functions of most Aux/IAAs have been defined mainly by gain-of-function mutant alleles in Arabidopsis thaliana, phenotypes associated with loss-of-function mutations have been scarce and subtle. We report here that the downregulation of IAA9, a tomato (Solanum lycopersicum) gene from a distinct subfamily of Aux/IAA genes, results in a pleiotropic phenotype, consistent with its ubiquitous expression pattern. IAA9-inhibited lines have simple leaves instead of wild-type compound leaves, and fruit development is triggered before fertilization, giving rise to parthenocarpy. This indicates that IAA9 is a key mediator of leaf morphogenesis and fruit set. In addition, antisense plants displayed auxin-related growth alterations, including enhanced hypocotyl/stem elongation, increased leaf vascularization, and reduced apical dominance. Auxin dose–response assays revealed that IAA9 downregulated lines were hypersensitive to auxin, although the only early auxin-responsive gene that was found to be upregulated in the antisense lines was IAA3. The activity of the IAA3 promoter was stimulated in the IAA9 antisense genetic background, indicating that IAA9 acts in planta as a transcriptional repressor of auxin signaling. While no mutation in any member of subfamily IV has been reported to date, the phenotypes associated with the downregulation of IAA9 reveal distinct and novel roles for members of the Aux/IAA gene family.

Từ khóa


Tài liệu tham khảo

1995, J. Mol. Biol., 251, 533, 10.1006/jmbi.1995.0454

1994, Plant J., 5, 421, 10.1111/j.1365-313X.1994.00421.x

1988, J. Biol. Chem., 263, 10658, 10.1016/S0021-9258(18)38022-0

2003, Plant Physiol., 131, 186, 10.1104/pp.010132

1974, J. Am. Soc. Hortic. Sci., 99, 385, 10.21273/JASHS.99.5.385

2003, Planta, 217, 726, 10.1007/s00425-003-1052-1

1998, Plant Cell Physiol., 39, 993, 10.1093/oxfordjournals.pcp.a029311

2001, Curr. Opin. Plant Biol., 4, 50, 10.1016/S1369-5266(00)00135-7

2005, Nature, 435, 441, 10.1038/nature03543

1999, Mol. Breed., 5, 463, 10.1023/A:1009665409959

2000, Plant Physiol., 122, 471, 10.1104/pp.122.2.471

2001, Physiol. Plant, 111, 545, 10.1034/j.1399-3054.2001.1110416.x

2003, Curr. Opin. Plant Biol., 6, 7, 10.1016/S1369526602000031

2003, Nature, 421, 740, 10.1038/nature01387

2002, Plant J., 29, 153, 10.1046/j.0960-7412.2001.01201.x

1998, Science, 282, 2226, 10.1126/science.282.5397.2226

1993, Plant Cell, 5, 1439, 10.2307/3869794

1937, Am. J. Bot., 24, 102, 10.1002/j.1537-2197.1937.tb09075.x

2002, Genes Dev., 16, 1610, 10.1101/gad.229402

1999, Development, 126, 1387, 10.1242/dev.126.7.1387

1996, Cell, 84, 735, 10.1016/S0092-8674(00)81051-X

2000, Plant Mol. Biol., 42, 819, 10.1023/A:1006496308160

2000, Development, 127, 23, 10.1242/dev.127.1.23

2002, Plant J., 32, 603, 10.1046/j.1365-313X.2002.01450.x

2005, Nature, 435, 446, 10.1038/nature03542

2004, Curr. Opin. Plant Biol., 7, 65, 10.1016/j.pbi.2003.11.002

1996, Plant J., 15, 441

1997, Proc. Natl. Acad. Sci. USA, 94, 11786, 10.1073/pnas.94.22.11786

2005, Development, 132, 1699, 10.1242/dev.01716

2005, J. Exp. Bot., 56, 25

2002, Plant Mol. Biol., 49, 387, 10.1023/A:1015255030047

1978, Plant Cell Physiol., 19, 1281

2003, Plant Physiol., 131, 1327, 10.1104/pp.013623

1999, Development, 126, 2979, 10.1242/dev.126.13.2979

2000, Plant Physiol., 123, 563, 10.1104/pp.123.2.563

2000, Plant Mol. Biol., 44, 73, 10.1023/A:1006437205596

2001, Plant Cell, 13, 829, 10.1105/tpc.13.4.829

2002, Plant J., 32, 669, 10.1046/j.1365-313X.2002.01459.x

1996, Planta, 200, 229

2001, Trends Plant Sci., 6, 420, 10.1016/S1360-1385(01)02042-8

2001, Plant Cell, 13, 465, 10.1105/tpc.13.3.465

2000, Plant J., 21, 547, 10.1046/j.1365-313x.2000.00702.x

1998, Science, 279, 1371, 10.1126/science.279.5355.1371

2000, Plant Cell Physiol., 41, 649, 10.1093/pcp/41.6.649

2002, Brief. Bioinform., 3, 246, 10.1093/bib/3.3.246

2004, Plant Cell, 16, 379, 10.1105/tpc.018630

1999, Development, 126, 711, 10.1242/dev.126.4.711

1994, Genetics, 138, 1239, 10.1093/genetics/138.4.1239

2004, Plant Cell, 16, 533, 10.1105/tpc.017384

1997, Science, 276, 1865, 10.1126/science.276.5320.1865

2003, Curr. Opin. Plant Biol., 6, 51, 10.1016/S1369-5266(02)00013-4

2004, Plant J., 40, 772, 10.1111/j.1365-313X.2004.02254.x

1999, Plant J., 18, 589, 10.1046/j.1365-313x.1999.00483.x

Thông tin
Thông tin xuất bản

Plant Cell

Tập 17 Số 10

2676-2692

Thông tin tác giả