The Master Quorum-Sensing Regulator OpaR is Activated Indirectly by H-NS in Vibrio parahaemolyticus
Tóm tắt
AphA and OpaR are the master regulators of quorum sensing in Vibrio parahaemolyticus and there is reciprocal negative regulation between AphA and OpaR. The histone-like nucleoid structure (H-NS) protein is a global transcriptional repressor of horizontally transferred genes, but a few prokaryotic genes, for example flagella genes, are stimulated by H-NS. The regulation of opaR by H-NS was investigated using the following methods: primer extension, LacZ fusion, quantitative RT-PCR, and electrophoretic mobility shift assay. One transcription start site located at 74 bp upstream of opaR was detected and its activity was induced by H-NS. Therefore, a single H-NS-dependent promoter was transcribed for opaR in V. parahaemolyticus. Because the H-NS protein could not bind to the upstream region of opaR and H-NS does not regulate aphA, indirect activation of the transcription of opaR by H-NS cannot be mediated by the AphA repressor.
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