The Influence of Low Platelet Count on Whole Blood Aggregometry Assessed by Multiplate

Clinical and Applied Thrombosis/Hemostasis - Tập 17 Số 6 - Trang E211-E217 - 2011
Trine Stissing1, Nadia Dridi2, Sisse Rye Ostrowski1, Louise Bochsen1, Pär I. Johansson1
1Department of Clinical Immunology, Section for Transfusion Medicine, Capital Region Blood Bank, Rigshospitalet 2034, University of Copenhagen, Denmark
2Department of Cardiology, 2013, Rigshospitalet, University of Copenhagen, Denmark

Tóm tắt

The Multiplate, a whole blood (WB) platelet function test, has shown promising results identifying patients on antiplatelet therapy at increased risk of rethrombosis. In the present study, the influence of low platelet count on platelet aggregation was analyzed and compared with aggregation results in an artificial matrix, platelet-rich plasma (PRP). Heparinized and citrated blood was diluted with autologous plasma to platelet concentrations 200 to 25 × 109/L in WB samples (n = 10) and 200 to 100 × 109/L in PRP samples (n = 7). The platelet aggregation was investigated by the ADP-, ASPI-, COL-, and TRAP-test. The WB responses decreased at platelet concentration of ≤100 × 109/L (all P < .03), except for heparin-TRAP (50 × 109/L, P = .008) and citrate-ASPI (150 × 109/L, P = .03). In general, WB samples demonstrated higher aggregation than PRP samples at platelet concentrations 200 to 100 × 109/L ( P < .05). In conclusion, platelet concentration of <150 × 109/L may influence Multiplate which should be considered in clinical settings. Furthermore, the findings emphasize the importance of evaluating haemostasis in its natural matrix, WB.

Từ khóa


Tài liệu tham khảo

Smith SC, 2006, Circulation, 113, e166

10.1016/j.jacc.2005.07.041

10.1016/S0140-6736(01)05701-4

10.1001/jama.288.19.2411

10.1161/01.CIR.0000130846.46168.03

Mueller T, 2008, Clin Appl Thrombosis/Hemostasis, 1

10.1160/TH07-07-0478

10.1016/j.jacc.2008.11.030

10.1016/0160-5402(80)90024-8

10.1111/j.1399-6576.2008.01829.x

Calatzis A, 2004, Platelets, 15, 485

Tóth O, 2006, Thromb Haemost, 96, 781

10.1136/jcp.22.1.28

10.1136/jcp.37.8.874

10.1080/09537100802364076

10.1080/09537100600944277

10.1055/s-0037-1614486

10.1007/BF03022251

10.1161/01.ATV.0000031340.68494.34

10.3324/haematol.10999

10.1161/01.ATV.0000089330.88461.D6

10.1111/j.1537-2995.2010.02681.x

Bochsen L, Blood Coagulation Fibrinolysis

10.1186/2047-783X-15-5-214

10.1097/00002371-199110000-00008

10.1016/0306-3623(94)00280-Z

10.1172/JCI116709

Thông tin
Thông tin xuất bản

Clinical and Applied Thrombosis/Hemostasis

Tập 17 Số 6

E211-E217

Thông tin tác giả