The Enhancement Effect of Calcium Ions on Ectomycorrhizal Fungi-Mediated Drought Resistance in Pinus sylvestris var. mongolica
Tóm tắt
This study evaluated the overall influence of ectomycorrhizal fungi (ECMF) and exogenous Ca2+ supplementation on the growth, physiological, and metabolic traits of Pinus sylvestris var. mongolica seedlings under water stress and well-watered conditions. Several analytical methodologies pertinent to mycology, biochemistry, and ecology were applied. Exogenous Ca2+ was applied to 1-year-old mycorrhizal P. sylvestris var. mongolica, and the growth and physiological traits of various parts of P. sylvestris var. mongolica were assessed under water stress and well-watered conditions. The results showed that Ca2+ has an additional effect on the growth of P. sylvestris var. mongolica ECMF-inoculated under water stress. Ca2+ and ECMF together can enhance the activity of antioxidant enzymes, such as CAT, POD, SOD, and APX in the seedlings, and resist the accumulation of reactive oxygen species (ROS) caused by water stress. An appropriate combination of Ca2+ and ECMF can reduce the MDA content more efficiently in P. sylvestris var. mongolica and alleviate the extent of cellular stress. However, whether the free proline content is related to ECMF/Ca2+ inoculation remains to be further studied. The current findings indicate that, in the early stage of seedling development, it is feasible to apply exogenous Ca2+ and ECMF to improve growth and drought resistance.
Tài liệu tham khảo
Allen CD, Macalady AK, Chenchouni H et al (2010) A global overview of drought and heat-induced tree mortality reveals emerging climate change risks for forests. For Ecol Manag 259(4):660–684
Baar J, Stanton NL (2000) Ectomycorrhizal fungi challenged by saprotrophic basidiomycetes and soil microfungi under different ammonium regimes in vitro. Mycol Res 104:691–697
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207
Breshears DD, Cobb NS, Rich PM et al (2005) Regional vegetation die-off in response to global-change-type drought. Proc Natl Acad Sci USA 102(42):15144–15148
Chen JP, Lee MS (1994) Simultaneous production and partition of chitinase during growth of Serratia marcescens in an aqueous two phase system. Biotechnol Tech 8:783–788
De I, Cruz J, Pintor-Toro J, Benitez JA, Llobell T, Romero A (1995) A novel endo-β-1,3-glucanase, BGN131, involved in the mycoparasitism of Trichoderma harzianum. J Bacteriol 177:6937–6945
Edda S, Oddsdottir ES, Eilenberg J, Sen R, Halldorsson G (2010) The effects of insect pathogenic soil fungi and ectomycorrhizal inoculation of birch seedlings on the survival of Otiorhynchus larvae. Agric Entomol 12:319–324
Gauthier S, Bernier P, Kuuluvainen T et al (2015) Boreal forest health and global change. Science 349(6250):819–822
Griffiths RP, Baham JE, Caldwell B (1994) A Soil solution chernistry of ectomyeorrhizal mats in forest. Soil Biol Biochem 26:331–337
He L, Qin JJ, Long LY (2011) Net cadmium flux and accumulation reveal tissue-specific oxidative stress and detoxification in Populus×canescens. Physiol Plant 143(1):50–63
Hirschi KD (2004) The calcium conundrum. Both versatile nutrient and specific signal. Plant Physiol 136(1):2438–2442
Hsiao TC (1973) Plant response to water stress. Ann Rev Plant Physiol 24:519–570
Huang Y, Jiang XY, Liang ZC, Li T (2006) Effect of ectomycorrhizal fungi on growth and physiology of Pinus tabulaeformis seedlings under saline stress. J Agro Environ 25(6):1475–1480
Huang Z, Zou ZR, He CX, He ZQ, Zhang ZB, Li JM (2011) Physiological and photosynthetic responses of melon (Cucumis melo L.) seedlings to three glomus species under water deficit. Plant Soil 339:391–399
Jaleel CA, Riadh K, Gopi R, Manivannan P, Al-Jaburi HJ, Zhao CX, Shao HB, Panneerselvam R (2009) Antioxidant defense responses: physiological plasticity in higher plants under abiotic constraints. Acta Physiol Plant 31:427–436
Johansson I, Larsson C, Kjellbom P (1996) The major integral proteins of spinach leaf plasma membranes are putative aquaporins and are phosphorylated in response to Ca2+ and apo-plastic water potential. Plant Cell 89(7):1181–1191
Li SP, Bi YL, Chen PZ, Zhakypbek Y, Liu S (2013) Effects of AMF cooperating with exogenous calcium on maize growth and soil improvement. Trans Chin Soc Agric Eng 29(1):109–116
Lugo AE (2015) Forestry in the Anthropocene. Science 349(6250):771
Mauch F, Mauch-Mani B, Boller T (1988) Antifungal hydrolases in peatissue. II Inhibition of fungal growth by combination of chitinase and β-1,3-glucanase. Plant Physiol 88:936–942
Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic Press, New York
Mucha J, Dahm H, Strzelczyk E, Werner A (2006) Synthesis of enzymes connected with mycoparasitism by ectomycorrhizal fungi. Arch Microbiol 185:69–77
Ravi S, Breshears DD, Huxman TE (2010) Land degradation in drylands: interactions among hydrologic-aeolian erosion and vegetation dynamics. Geomorphology 116:236–245
Ruiz-Lozano JM (2003) Arbuscular mycorrhizal symbiosis and alleviation of osmotic stress. New perspectives for molecular studies. Mycorrhiza 13:309–317
Sanders D, Brownlee C, Harper JF (1999) Communicating with calcium. Plant Cell 11(4):691–706
Sharma R, Rajak RC, Pandey AK (2010) Evidence of antagonistic interactions between rhizosphere and mycorrhizal fungi associated with Dendrocalamus strictus (Bamboo). J Yeast Fungal Res 1:112–117
Shaw TM, Dighton J, Sanders FE (1995) Interactions between ectomycorrhizal and saprotrophic fungi on agar and in association with seedlings of lodgepole pine (Pinus contorta). Mycol Res 99:159–165
Shinozaki K, Yamaguchi K (1997) Gene expression and signal transduction in water-stress response. Plant Physiol 115(2):327–334
Song LN, Zhu JJ, Li MC et al (2014) Water utilization of Pinus sylvestris var. mongolica in a sparse wood grassland in the semi-arid sandy region of Northeast China. Trees 28:971–982
Song LN, Zhu JJ, Yan QL (2015) Comparison of intrinsic water use efficiency between different aged Pinus sylvestris var. mongolica wide windbreaks in semi-arid sandy land of northern China. Agrofor Syst 89:477–489
Song LN (2016) Water use patterns of Pinus sylvestris var. mongolica trees of different ages in a semi-arid sandy lands of Northeast China. Environ Exper Bot 129:94–107
Song LN, Zhu JJ, Li MC et al (2016) Sources of water used by Pinus sylvestris var. mongolica trees based on stable isotope measurements in a semi-arid sandy region of Northeast China. Agric Water Manag 164:281–290
Song LN, Zhu JJ, Zheng X (2017) Forestation and management scheme of Pinus sylvestris var. mongolica plantations in sandy lands based on their decline mechanisms. Chin J Ecol 36(11):3249–3256
Sugden A, Fahrenkamp-Uppenbrink J, Malakoff D et al (2015) Forest health in a changing world. Science 349(6250):800–801
Trumbore S, Brando P, Hartmann H (2015) Forest health and global change. Science 349(6250):814–818
Vinale F, D’Ambrosio G, Abadi K, Scala F, Marra R, Turra D, Woo SL, Lorito M (2004) Application of Trichoderma harzianum (T22) and Trichoderma atroviride (P1) as plant growth promoters, and their compatibility with copper oxychloride. J Z J Univ Sci 30:2–8
Wasowicz W, Jean N, Peratz A (1993) Optimized steps in fluorometric determination of thiobarbituric acid reactive substances in serum; importance of extraction pH and influence of sample preservation and storage. Clin Chem 38(12):2522–2526
Wang XM, Chen F, Hasi E, Li JC (2008) Desertification in China: an assessment. Earth Sci Rev 88:188–206
Wu XQ, Sun MQ, Gao Y, Sheng JM, Ye JR (2007) Effects of some ectomycorrhizas on pine seedlings to disease resistance. Sci Silva Sin 43(6):88–93
Wu XY, Jiang FQ, Li XD (2004) Major features of decline of Pinus sylvestris var. mongolica plantation on sandy land. Chin J Appl Ecol 15(12):2221–2224
Yang XH, Zhang KB, Jia BQ, Ci LJ (2005) Desertification assessment in China: an overview. J Arid Environ 63:517–531
Yin DC, Deng X, Chet I, Song RQ (2014) Physiological responses of Pinus sylvestris var. Mongolica seedlings to the interaction between Suillus luteus and Trichoderma virens. Curr Microbiol 69:334–342
Yin DC, Deng X, Song RQ (2016) Synergistic effects between Suillus luteus and Trichoderma virens on Korean spruce seedlings growth and drought resistance to Scotch pine seedlings. For Res 27(1):193–201
Yin DC, Qi JY, Deng JF, Du H, Deng X (2017) Effects of ectomycorrhizal cooperating with exogenous calcium on Pinus sylvestris var. mongolica growth. China Environ Sci 37(6):2295–2304
Yin DC, Song RQ, Qi JY, Deng X (2018) Ectomycorrhizal fungus enhances drought tolerance of Pinus sylvestris var. mongolica seedlings and improves soil condition. For Res 29(6):1775–1788
Yin DC, Saiyaremu HF, Song RQ, Qi JY, Deng X, Deng JF (2020) Effects of an ectomycorrhizal fungus on the growth and physiology of Pinus sylvestris var. mongolica seedlings subjected to saline–alkali stress. For Res 31(3):781–788
Zhang Y, Zhong CL, Chen Y, Chen Z, Jiang QB, Wu C, Pinyopusarerk K (2010) Improving drought tolerance of Causarina equisetifolia seedlings by arbuscular mycorrhizas under glasshouse conditions. New For 40:261–271
Zeng DH, Jiang FQ, Fan ZP (1996) Stability of Mongolian pine plantationson sandy land. Chin J Appl Ecol 7(4):337–343
Zheng X et al (2012) Effects of land use changes on the groundwater table and the decline of Pinus sylvestris var. mongolica plantations in southern Horqin Sandy Land, Northeast China. Agric Water Manag 109:94–106
Zheng X, Zhu JJ, Yan QL (2013) Monthly air temperatures over Northern China estimated by integrating MODIS data with GIS techniques. Appl Mete Cli 52:1987–2000
Zhu JJ, Fan ZP, Zeng DH et al (2003) Comparison of stand structure and growth between artificial and natural forests of Pinus sylvestiris var. mongolica on sandy land. For Res 14:103–111
Zhu JJ, Kang HZ, Tan H et al (2005) Natural regeneration characteristics of Pinus sylvestris var. mongolica forests on sandy land in Honghuaerji, China. For Res 16:253–259
Zhu JJ, Kang HZ, Tan H et al (2006a) Effects of drought stresses induced by polyethylene glycol on germination of Pinus sylvestris var. mongolica seeds from natural and plantation forests on sandy land. For Res 11:319–328
Zhu JJ, Tan H, Kang HZ et al (2006b) Comparison of foliar nutrient concentrations between natural and artificial forests of Pinus sylvestris var. mongolica on sandy land. China For Res 17:177–184
Zhu Y, He C, Du W et al (2007) Effects of exogenous calcum on the seed germination and seedling ions distribution of Festuca arundinacea under salt-stress. Tran of Chi Soci of AgriEngin 23(11):133–137
Zhu JJ et al (2008) The role of ectomycorrhizal fungi in alleviating pine decline in the semi-arid sandy soil of northern China: an experimental approach. Ann For Sci 65:304