The Effect of Nonsurgical Periodontal Therapy on HNP1-3 Level in Gingival Crevicular Fluid of Chronic Periodontitis Patients

Archivum Immunologiae et Therapiae Experimentalis - Tập 65 - Trang 355-361 - 2017
Ewa Dolińska1, Anna Skurska1, Małgorzata Pietruska1,2, Violetta Dymicka-Piekarska3, Robert Milewski4, Jan Pietruski2, Anton Sculean5
1Department of Periodontal and Oral Mucosa Diseases, Medical University of Bialystok, Białystok, Poland
2Private Practice, Białystok, Poland
3Department of Clinical Laboratory Diagnostics, Medical University of Bialystok, Białystok, Poland
4Department of Statistics and Medical Informatics, Medical University of Bialystok, Białystok, Poland
5Department of Periodontology, Dental School, University of Bern, Bern, Switzerland

Tóm tắt

The rich bacterial flora of oral cavity is controlled by innate immune response, including antibacterial peptides and among them human neutrophil peptides 1–3 (HNP1-3). The knowledge of the involvement of HNPs in innate and acquired immunity of the periodontium is fragmentary. The aim of the study was to assess alterations in HNP1-3 levels in the gingival crevicular fluid (GCF) of chronic periodontitis patients before and after nonsurgical periodontal therapy. Nineteen patients with chronic periodontitis were qualified to the study. After periodontal examination, one site with pocket depth (PD) ≥4 mm was selected. All the patients received periodontal treatment involving scaling and root planing with additional systemic antibiotic therapy (Amoxicillin 375 mg three times daily and Metronidazole 250 mg three times daily for 7 days). Prior to therapy, 3 and 6 months after it, clinical periodontal parameters were measured and GCF was collected from previously chosen site. The level of HNP1-3 in GCF was determined by means of a commercially available enzyme-linked immunoassay kit. The periodontal therapy caused a statistically significant (p < 0.001) decrease in all the assessed clinical parameters at the sites of sample collection except for bleeding on probing. The level of HNP1-3 per measure point showed a statistically significant increase (baseline—3 months: p = 0.05, baseline—6 months: p = 0.007). Within the limits of the study, it can be stated that nonsurgical periodontal therapy with additional systemic administration of Amoxicillin and Metronidazole increases the level of HNP1-3 in GCF.

Tài liệu tham khảo

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