The Complete Genome Sequence of a Gossypol-Degrading Bacterial Strain, Raoultella sp. YL01
Tóm tắt
Cottonseed meal is an important source of plant protein for the meal fodder materials. But its usage in animal breeding industry is limited by a type of toxic phenol, gossypol, that has toxic effects on animal health. Microbial degradation is a promising way to lower down gossypol in cottonseed meal. However, the molecular mechanisms of bio-degradation of gossypol is still unclear. In this study we isolated a gossypol-degrading bacterial strain, YL01, and sequenced its complete genome via Oxford Nanopore sequencing method. There is a chromosome (5,737,005 bp) and a plasmid (136,446 bp) in YL01. 5489 protein coding genes in total were functionally annotated. 16S rRNA analysis showed that YL01 taxonomically belongs to the genus of Raoultella. YL01 is the first published complete genome sequence of microbes capable of gossypol degradation. Gene function annotation showed that 126 protein coding genes may involve in gossypol catabolism. Sequence similarity analysis showed that, as the only gossypol-degrading strain in the genus of Raoultella, YL01 uniquely holds 260 genes that are not possessed by other Raoultella strains. Our work gives a preliminary list for genes responsible for gossypol degradation but further investigations are needed to completely disclose this molecular processes.
Tài liệu tham khảo
Kenar JA (2006) Reaction chemistry of gossypol and its derivatives. J Amer Oil Chem Soc 83:269–302. https://doi.org/10.1002/chin.200721239
Alexander J, Benford D, Cockburn A et al (2008) Gossypol as undesirable substance in animal feed—scientific opinion of the panel on contaminants in the food chain. Euro Food Saf Auth J 908:1–55. https://doi.org/10.1002/chin.200721239
Santos J, Villasenor M, Robinson PH et al (2003) Type of cottonseed and level of gossypol in diets of lactating dairy cows: plasma gossypol, health, and reproductive performance. J Dairy Sci 86:892–905. https://doi.org/10.3168/jds.S0022-0302(03)73672-8
Gadelha I, Fonseca N, Oloris S et al (2014) Gossypol toxicity from cottonseed products. Sci World J 2014:231635. https://doi.org/10.1155/2014/231635
Randel RD, Chase C, Wyse SJ (1992) Effects of gossypol and cottonseed products on reproduction of mammals. J Anim Sci 70:1628–1638. https://doi.org/10.1080/00071669208417483
Lv Y, Wang X, Zhao Q et al (2010) Research situation on gossypol safety limit in feed and gossypol residues in livestock product. Chin Agric Sci Bull 26:1–5
Wu DD (1989) An overview of the clinical pharmacology and therapeutic potential of gossypol as a male contraceptive agent and in gynaecological disease. Drugs 38:333–341. https://doi.org/10.2165/00003495-198938030-00001
Coutinho EM (2002) Gossypol: a contraceptive for men. Contraception 65:259–263. https://doi.org/10.1016/s0010-7824(02)00294-9
Zubair H, Khan HY, Ullah MF et al (2012) Apogossypolone, derivative of gossypol, mobilizes endogenous copper in human peripheral lymphocytes leading to oxidative DNA breakage. Eur J Pharm Sci 47:280–286. https://doi.org/10.1016/j.ejps.2012.04.014
Goetsch AL, Owens FN (1985) The effects of commercial processing method of cottonseed meal on site and extent of digestion in cattle. J Anim Sci 60:803–813. https://doi.org/10.2527/jas1985.603803x
Rahma EH, Rao M (1984) Gossypol removal and functional properties of protein produced by extraction of glanded cottonseed with different solvents. J Food Sci 49:1057–1060. https://doi.org/10.1111/j.1365-2621.1984.tb10391.x
Barraza ML, Coppock CE, Brooks KN et al (1991) Iron sulfate and feed pelleting to detoxify free gossypol in cottonseed diets for dairy cattle. J Dairy Sci 74:3457–3467. https://doi.org/10.3168/jds.S0022-0302(91)78536-6
Nagalakshmi D, Sastry V, Agrawal DK (2002) Detoxification of undecorticated cottonseed meal by various physical and chemical methods. Anim nutr feed techn 2:117–126
Zhang Y, Zhang Z, Dai L et al (2018) Isolation and characterization of a novel gossypol-degrading bacteria Bacillus subtilis strain Rumen Bacillus Subtilis. Asian Austral J Anim Sci 31:63–70. https://doi.org/10.5713/ajas.17.0018
Wang XL, Liu Q, Han W et al (2016) Screening of gossypol- removing strain and the fermentation of cottonseed meal with mixed culture. Sci Technol Cereals, Oils Foods 24:81–85
Weng XY, Sun JY (2006) Biodegradation of free gossypol by a new strain of Candida tropicalis under solid state fermentation: effects of fermentation parameters. Process Biochem 41:1663–1668. https://doi.org/10.1016/j.procbio.2006.03.015
Weng XY, Sun JY (2006) Kinetics of biodegradation of free gossypol by Candida tropicalis in solid-state fermentation. Biochem Eng J 32:226–232. https://doi.org/10.1016/j.bej.2006.10.007
Sun ZC, Fang HY, Guo JL et al (2011) Isolation, identification and mutation breeding of high gossypol detoxification strain. Microbiology China 38:1166–1171. https://doi.org/10.1007/s11606-010-1494-7
Zhang WJ, Xu ZR, Zhao SH et al (2007) Development of a microbial fermentation process for detoxification of gossypol in cottonseed meal. Ani Feed Sci Tech 135:176–186. https://doi.org/10.1016/j.anifeedsci.2006.06.003
Yang X, Sun JY, Guo JL et al (2012) Identification and proteomic analysis of a novel gossypol-degrading fungal strain. J Sci Food Agr 92:943–951. https://doi.org/10.1002/jsfa.4675
Rajarathnam S, Shashirekha MN, Bano Z (2001) Biodegradation of gossypol by the white oyster mushroom, Pleurotus florida, during culturing on rice straw growth substrate, supplemented with cottonseed powder. World J Microb Biot 17:221–227. https://doi.org/10.1023/A:1016603510901
Feng WANG, Weishu ZHANG, Ying CUI et al (2022) Screening and identification of gossypol-degrading bacteria and studies on solid state fermentation of cottonseed meal[J]. Sci Technol Food Ind 43(17):132–139. https://doi.org/10.13386/j.issn1002-0306.2021100143
Weisburg WG, Barns SM, Pelletier DA et al (1991) 16S ribosomal DNA amplifcation for phylogenetic study. J Bacteriol 173:697–703. https://doi.org/10.1128/jb.173.2.697-703.1991
Sala-Trepat JM, Murray K, Williams PA (1972) The metabolic divergence in the meta cleavage of catechols by Pseudomonas putida NCIB 10015: physiological significance and evolutionary implications. Eur J Biochem 28:347–356. https://doi.org/10.1111/j.1432-1033.1972.tb01920.x
Murray K, Duggleby CJ, Williams PA et al (1972) The metabolism of benzoate and methylbenzoates via the meta-cleavage pathway by Pseudomonas arvilla mt-2. Eur J Biochem 28:301–310. https://doi.org/10.1111/j.1432-1033.1972.tb01914.x
Mayer RJ, Que L (1984) 18O studies of pyrogallol cleavage by catechol 1,2-dioxygenase. J Biol Chem 259:13056–13060. https://doi.org/10.1016/s0021-9258(18)90655-1
Zylstra GJ, Olsen RH, Ballou DP (1989) Cloning, expression, and regulation of the Pseudomonas cepacia protocatechuate 3,4-dioxygenase genes. J Bacteriol 171:5907–5914. https://doi.org/10.1128/jb.171.11.5907-5914.1989
Williams PA, Sayers JR (1994) The evolution of pathways for aromatic hydrocarbon oxidation in Pseudomonas. Biodegradation 5:195–217. https://doi.org/10.1007/BF00696460