The Campylobacter jejuni/coli cjaA (cj0982c) Gene Encodes an N-Glycosylated Lipoprotein Localized in the Inner Membrane
Tóm tắt
The Campylobacter coli 72Dz/92 cjaA gene (orthologue of cj0982c of C. jejuni NCTC 11168) product is a highly immunogenic, amino acid–binding protein. CjaA was palmitic acid-modified when processed in E. coli. In addition, site-directed mutagenesis of the Cys residue of the LAAC motif of its signal sequence confirmed that CjaA is a lipoprotein when processed in Campylobacter. Localization of the protein appeared to be host dependent. In Campylobacter, CjaA was recovered mainly as an inner-membrane protein, whereas in E. coli most of the protein was present in the periplasmic space. Interestingly, antiserum raised against Campylobacter glycine-extracted material also recognized CjaA produced by Campylobacter and Escherichia coli, indicating that at least part of the protein may be surface exposed. Site-directed mutagenesis of the Asn residues of two putative N-linked glycosylation sites (NIS and NFT) showed that CjaA is glycosylated and that only the first N-X-S/T sequeon serves as a glycan acceptor.
Tài liệu tham khảo
Altekruse SF, Stern NJ, Fields PI et al (1999) Campylobacter jejuni – an emerging foodborne pathogen. Emerg Infect Dis 5:28–35
Ames GF, Prody C, Kustu S (1984) Simple, rapid, and quantitative release of periplasmic proteins by chloroform. J Bacteriol 160:1181–1183
Blaser MJ, Hopkins JA, Berka RM et al (1983) Identification and characterization of Campylobacter jejuni outer membrane proteins. Infect Immun 42:276–284
Bolla JM, De E, Dorez A et al (2000) Purification, characterization and sequence analysis of Omp50, a new porin isolated from Campylobacter jejuni. Biochem J 352(Part 3):637–643
Coker AO, Isokpehi RD, Thomas BN et al (2002) Human campylobacteriosis in developing countries. Emerg Infect Dis 8:237–244
Filip C, Fletcher G, Wulff JL et al (1973) Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol 115:717–722
Fouts DE, Mongodin EF, Mandrell RE et al (2005) Major structural differences and novel potential virulence mechanisms from the genomes of multiple campylobacter species. PLoS Biol 3(1):e15
Garvis SG, Puzon GJ, Konkel ME (1996) Molecular characterization of a Campylobacter jejuni 29-kilodalton periplasmic binding protein. Infect Immun 64:3537–3543
Higgins CF (2001) ABC transporters: physiology, structure and mechanism – an overview. Res Microbiol 152:205–210
Hosie AH, Poole PS (2001) Bacterial ABC transporters of amino acids. Res Microbiol 152:259–270
Jin S, Joe A, Lynett J et al (2001) JlpA, a novel surface-exposed lipoprotein specific to Campylobacter jejuni, mediates adherence to host epithelial cells. Mol Microbiol 39:1225–1236
Karlyshev AV, Ketley JM, Wren BW (2005) The Campylobacter jejuni glycome. FEMS Microbiol Rev 29:377–390
Kelly DJ (2001) The physiology and metabolism of Campylobacter jejuni and Helicobacter pylori. Symp Ser Soc Appl Microbiol 30:16S–24S
Korlath JA, Osterholm MT, Judy LA et al (1985) A point-source outbreak of campylobacteriosis associated with consumption of raw milk. J Infect Dis 152:592–596
Kowarik M, Young NM, Numao S et al (2006) Definition of the bacterial N-glycosylation site consensus sequence. EMBO J 25:1957–1966
Larsen JC, Szymanski C, Guerry P (2004) N-linked protein glycosylation is required for full competence in Campylobacter jejuni 81-176. J Bacteriol 186:6508–6514
Linton D, Allan E, Karlyshev AV, Cronshaw AD et al (2002) Identification of N-acetylgalactosamine-containing glycoproteins PEB3 and CgpA in Campylobacter jejuni. Mol Microbiol 43:497–508
Linton D, Dorrell N, Hitchen PG et al (2005) Functional analysis of the Campylobacter jejuni N-linked protein glycosylation pathway. Mol Microbiol 55:1695–1703
Masuda K, Matsuyama S, Tokuda H (2002) Elucidation of the function of lipoprotein-sorting signals that determine membrane localization. Proc Natl Acad Sci U S A 99:7390–7395
Muller A, Leon-Kempis Mdel R, Dodson E et al (2007) A bacterial virulence factor with a dual role as an adhesin and a solute-binding protein: The crystal structure at 1.5 A resolution of the PEB1a protein from the food-borne human pathogen Campylobacter jejuni. J Mol Biol 372:160–171
Muller A, Thomas GH, Horler R et al (2005) An ATP-binding cassette-type cysteine transporter in Campylobacter jejuni inferred from the structure of an extracytoplasmic solute receptor protein. Mol Microbiol 57:143–155
Nachamkin I (2002) Chronic effects of Campylobacter infection. Microbes Infect 4:399–403
Nita-Lazar M, Wacker M, Schegg B et al (2005) The N-X-S/T consensus sequence is required but not sufficient for bacterial N-linked protein glycosylation. Glycobiology 15:361–367
On SL, Jordan PJ (2003) Evaluation of 11 PCR assays for species-level identification of Campylobacter jejuni and Campylobacter coli. J Clin Microbiol 41:330–336
Page WJ, Taylor DE (1988) Comparison of methods used to separate the inner and outer membranes of cell envelopes of Campylobacter spp. J Gen Microbiol 134:2925–2932
Pawelec D, Rozynek E, Popowski J et al (1997) Cloning and characterization of a Campylobacter jejuni 72Dz/92 gene encoding a 30 kDa immunopositive protein, component of the ABC transport system: Expression of the gene in avirulent Salmonella typhimurium. FEMS Immunol Med Microbiol 19:137–150
Pawelec DP, Korsak D, Wyszynska AK et al (2000) Genetic diversity of the Campylobacter genes coding immunodominant proteins. FEMS Microbiol Lett 185:43–49
Pei ZH, Ellison RT 3rd, Blaser MJ (1991) Identification, purification, and characterization of major antigenic proteins of Campylobacter jejuni. J Biol Chem 266:16363–16369
Pittman MS, Elvers KT, Lee L et al (2007) Growth of Campylobacter jejuni on nitrate and nitrite: electron transport to NapA and NrfA via NrfH and distinct roles for NrfA and the globin Cgb in protection against nitrosative stress. Mol Microbiol 63:575–590
Sambrook J, Russel DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York, NY
Skirrow MB, Blaser MJ (2000) Clinical aspects of Campylobacter infection. In: Nachamkin I, Blaser MJ, Tompkins LS (eds) Campylobacter, 2nd ed. ASM Press, Washington, DC, pp 69–89
Szymanski CM, Wren BW (2005) Protein glycosylation in bacterial mucosal pathogens. Nat Rev Microbiol 3:225–237
Szymanski CM, Yao R, Ewing CP et al (1999) Evidence for a system of general protein glycosylation in Campylobacter jejuni. Mol Microbiol 32:1022–1030
Velayudhan J, Kelly DJ (2002) Analysis of gluconeogenic and anaplerotic enzymes in Campylobacter jejuni: an essential role for phosphoenolpyruvate carboxykinase. Microbiology 148:685–694
Wacker M, Linton D, Hitchen PG et al (2002) N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli. Science 298:1790–1793
Wassenaar TM, Fry BN, van der Zeijst BA (1993) Genetic manipulation of Campylobacter: Evaluation of natural transformation and electro-transformation. Gene 132:131–135
Wyszynska A, Raczko A, Lis M et al (2004) Oral immunization of chickens with avirulent Salmonella vaccine strain carrying C. jejuni 72Dz/92 cjaA gene elicits specific humoral immune response associated with protection against challenge with wild-type Campylobacter. Vaccine 22:1379–1389
Wyszynska A, Tomczyk K, Jagusztyn-Krynicka EK (2007) Comparison of the localization and post-translational modification of Campylobacter coli CjaC and its homolog from Campylobacter jejuni, Cj0734c/HisJ. Acta Biochim Pol 54:143–150
Yakushi T, Yokota N, Matsuyama S et al (1998) LolA-dependent release of a lipid-modified protein from the inner membrane of Escherichia coli requires nucleoside triphosphate. J Biol Chem 273:32576–32581
Yamaguchi K, Yu F, Inouye M (1988) A single amino acid determinant of the membrane localization of lipoproteins in E. coli. Cell 53:423–432
Yao R, Alm RA, Trust TJ et al (1993) Construction of new Campylobacter cloning vectors and a new mutational cat cassette. Gene 130:127–130
Yokota N, Kuroda T, Matsuyama S et al (1999) Characterization of the LolA-LolB system as the general lipoprotein localization mechanism of Escherichia coli. J Biol Chem 274:30995–30999
Young NM, Brisson JR, Kelly J et al (2002) Structure of the N-linked glycan present on multiple glycoproteins in the Gram-negative bacterium, Campylobacter jejuni. J Biol Chem 277:42530–42539