The Campylobacter jejuni/coli cjaA (cj0982c) Gene Encodes an N-Glycosylated Lipoprotein Localized in the Inner Membrane

Current Microbiology - Tập 57 - Trang 181-188 - 2008
Agnieszka Wyszyńska1, Joanna Życka1,2, Renata Godlewska1, Elżbieta K. Jagusztyn-Krynicka1
1Department of Bacterial Genetics, Institute of Microbiology, University of Warsaw, Warsaw, Poland
2Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

Tóm tắt

The Campylobacter coli 72Dz/92 cjaA gene (orthologue of cj0982c of C. jejuni NCTC 11168) product is a highly immunogenic, amino acid–binding protein. CjaA was palmitic acid-modified when processed in E. coli. In addition, site-directed mutagenesis of the Cys residue of the LAAC motif of its signal sequence confirmed that CjaA is a lipoprotein when processed in Campylobacter. Localization of the protein appeared to be host dependent. In Campylobacter, CjaA was recovered mainly as an inner-membrane protein, whereas in E. coli most of the protein was present in the periplasmic space. Interestingly, antiserum raised against Campylobacter glycine-extracted material also recognized CjaA produced by Campylobacter and Escherichia coli, indicating that at least part of the protein may be surface exposed. Site-directed mutagenesis of the Asn residues of two putative N-linked glycosylation sites (NIS and NFT) showed that CjaA is glycosylated and that only the first N-X-S/T sequeon serves as a glycan acceptor.

Tài liệu tham khảo

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