Temperature-Induced Change of Variant Surface Antigen Expression in ParameciumInvolves Antigen Release into the Culture Medium with Considerable Delay betweenTranscription and Surface Expression
Tóm tắt
We analyzed temperature-induced changes of variant surface antigen (vsAg) expression in Paramecium primaurelia, using immuno-techniques and mRNA determinations. Upon a 23°C to 33°C shift, the old vsAg, type 156G, remains on the cell surface for a time, when already mRNA for the new form, 156D, is expressed. A considerable amount of 156D-specific mRNA is formed 45–48 h after the temperature shift, while 156D surface expression reaches maximal levels only after >72 h. A new aspect of these experiments is that, during this transition, the old vsAg is steadily released in high-molecular-weight form into the culture medium, as found by dot blot and Western blot analysis of concentrated culture medium. The new vsAg form is first inserted into the somatic cell membrane, before it spreads also into cilia. In the reverse transition, 33°C to 23°C, the adaptation on the level of transcription and surface expression is considerably faster. While we had previously shown, under steady-state conditions (constant temperature), the occurrence of a degradation pathway by endocytotic and phagocytotic ingestion of vsAg this may proceed in parallel to the steady release of old vsAg from the cell surface into the medium. Altogether these combined processes may facilitate the installation of the new vsAg type.
Tài liệu tham khảo
A Adoutte K.Y Ling S. Chang F. Huang C. Kung (1983) ArticleTitlePhysiological and mutational protein variations in the ciliary membrane of Paramecium Exp. Cell Res. 148 387–404 Occurrence Handle10.1016/0014-4827(83)90161-1 Occurrence Handle1:CAS:528:DyaL2cXjtFOk Occurrence Handle6628563
C. Antony Y. Capdeville (1989) ArticleTitleUneven distribution of surface antigens during antigenic variation in Paramecium primaurelia J. Cell Sci. 92 205–215
N. Assouz Y. Capdeville (1992) ArticleTitleStructural comparisons between the soluble and the GPI-anchored forms of the Paramecium temperature-specific 156G surface antigen Biol. Cell. 75 217–223 Occurrence Handle10.1016/0248-4900(92)90143-O Occurrence Handle1335331
A. Barnett E. Steers (1984) ArticleTitleAntibody-induced membrane fusion in Paramecium J. Cell Sci. 65 153–162 Occurrence Handle1:CAS:528:DyaL2cXmtVOmsLc%3D Occurrence Handle6371030
G.H. Beale (1954) The Genetics of Paramecium aurelia Cambridge University Press Cambridge, New York
A. Benwakrim A. Trémolière. J. Labarre Y. Capdeville (1998) ArticleTitleThe lipid moiety of the GPI-anchor of the major plasma membrane proteins in Paramecium primaurelia is a ceramide: variation of the amide-linked fatty acid composition as a function of growth Protist 149 39–50
M.J. Blackman (2000) ArticleTitleProteases involved in erythrocyte invasion by the malaria parasite: Function and potential as chemotherapeutic targets Curr. Drug Targets 1 59–83 Occurrence Handle10.2174/1389450003349461 Occurrence Handle1:CAS:528:DC%2BD3cXlt1WhtL8%3D Occurrence Handle11475536
L.K. Bleyman (1996) Ciliate genetics K. Hausmann P.C. Bradbury (Eds) Ciliates - Cells, Organisms Gustav Fischer Verlag Stuttgart, Jena 291–324
Y. Capdeville (2000) ArticleTitle Paramecium GPI proteins: variability of expression and localization Protist 151 161–169 Occurrence Handle1:CAS:528:DC%2BD3cXns1aqs7o%3D Occurrence Handle10965955
Y. Capdeville A. Benwakrim (1996) ArticleTitleThe major ciliary membrane proteins in Paramecium primaurelia are all glycosylphosphatidylinositol-anchored proteins Eur. J. Cell Biol. 70 339–346 Occurrence Handle1:CAS:528:DyaK28Xlt1amsL8%3D Occurrence Handle8864662
Y. Capdeville F. Caron C. Antony C. Deregnaucourt A.M. Keller (1987) ArticleTitleAllelic antigen and membrane-anchor epitopes of Paramecium primaurelia surface antigens J. Cell Sci. 88 553–562 Occurrence Handle1:CAS:528:DyaL1cXht12ktr0%3D
T.G. Clark Y. Gao J. Gaertig X. Wang G. Cheng (2001) ArticleTitleThe I-antigens of Ichthyophthirius multifiliis are GPI-anchored proteins J. Eukaryot. Microbiol. 48 332–337 Occurrence Handle1:CAS:528:DC%2BD3MXkvVOgtbc%3D Occurrence Handle11411842
F. Debierre-Grockiego N. Azzouz J. Schmidt J.F. Dubremetz H. Geyer R. Geyer R. Weingart R.R. Schmidt R.T. Schwarz (2003) ArticleTitleRoles of glycosylphosphatidylinositols of Toxoplasma gondii. Induction of tumor necrosis factor-a production in macrophages J. Biol. Chem. 278 32987–32993 Occurrence Handle10.1074/jbc.M304791200 Occurrence Handle1:CAS:528:DC%2BD3sXms1alsr8%3D Occurrence Handle12815041
C. Deregnaucourt (1992) ArticleTitleTurnover of the GPI-anchored surface antigen in Paramecium. Partial release of its acylated form into the culture medium Eur. J. Protistol 28 220–225
F. Dzierszinski M. Mortuaire M.F. Crebron-Delauw S. Tomavo (2000) ArticleTitleTargeted disruption of the glycosylphosphatidylinositol-anchored surface antigen SAG3 gene in Toxoplasma gondii decreases host cell adhesion and drastically reduces virulence in mice Mol. Microbiol. 37 574–582 Occurrence Handle10.1046/j.1365-2958.2000.02014.x Occurrence Handle1:CAS:528:DC%2BD3cXmtFCrsLo%3D Occurrence Handle10931351
M.A.J. Ferguson (1999) ArticleTitleThe structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research J. Cell Sci. 112 2799–2809 Occurrence Handle1:CAS:528:DyaK1MXmtlWqsrk%3D Occurrence Handle10444375
M. Flötenmeyer M. Momayezi H. Plattner (1999) ArticleTitleImmunolabeling analysis of biosynthetic and degradative pathways of cell surface components (glycocalyx) in Paramecium cells Eur. J. Cell Biol. 78 67–77 Occurrence Handle10082425
S.A. Howell I. Wells S.L. Fleck C. Kettleborough C.R. Collins M.J. Blackman (2003) ArticleTitleA single malaria merozoite serine protease mediates shedding of multiple surface proteins by juxtamembrane cleavage J. Biol. Chem. 278 23890–23898 Occurrence Handle10.1074/jbc.M302160200 Occurrence Handle1:CAS:528:DC%2BD3sXkslygurs%3D Occurrence Handle12686561
I.G. Jones (1965) ArticleTitleImmobilization antigen in heterozygous clones of Paramecium aurelia Nature 207 769 Occurrence Handle1:STN:280:CCmC2MnmsVY%3D Occurrence Handle5885929
J. Kusch H.J. Schmidt (2001) ArticleTitleGenetically controlled expression of surface variant antigens in free-living protozoa J. Membrane Biol. 180 101–109 Occurrence Handle10.1007/s002320010062 Occurrence Handle1:CAS:528:DC%2BD3MXisFaktrs%3D
S. Lillico M.C. Field P. Blundell G.H. Coombs J.C. Mottram (2003) ArticleTitleEssential roles for GPI-anchored proteins in african trypanosomes revealed using mutants deficient in GPI8 Mol. Biol. Cell. 14 1182–1194 Occurrence Handle10.1091/mbc.E02-03-0167 Occurrence Handle1:CAS:528:DC%2BD3sXislOqtLY%3D Occurrence Handle12631733
C.A. Paquette V. Rakochy A. Bush J.L. Houten ParticleVan (2001) ArticleTitleGlycophosphatidylinositol-anchored proteins in Paramecium tetraurelia: possible role in chemoresponse J. Exp. Biol. 204 2899–2910 Occurrence Handle1:CAS:528:DC%2BD3MXnt1GjtbY%3D Occurrence Handle11683443
J.R. Preer (1959) ArticleTitleStudies on the immobilization antigens of Paramecium. II. Isolation J. Immunol. 83 378–384 Occurrence Handle14434717
J.R. Preer L.B. Preer B.M. Rudman (1981) ArticleTitlemRNAs for the immobilization antigens of Paramecium Proc. Natl. Acad. Sci. USA 78 6776–6778 Occurrence Handle1:CAS:528:DyaL38XltVamtA%3D%3D
J.B. Preer L.B. Preer B. Rudman A. Barnett (1987) ArticleTitleMolecular biology of the genes for immobilization antigens in Paramecium J. Protozool 34 418–423 Occurrence Handle1:CAS:528:DyaL1cXht1yh Occurrence Handle3323480
J.L. Rosenbaum G.B. Witman (2002) ArticleTitleIntraflagellar transport Nature Rev. Mol. Cell Biol. 3 813–825 Occurrence Handle10.1038/nrm952 Occurrence Handle1:CAS:528:DC%2BD38Xotlerur0%3D
T.M. Sonneborn (1947) ArticleTitleDevelopment mechanisms in Paramecium Growth Symp. 11 291–307
H. Webb N. Carnall L. Vanhamme S. Rolin J. Abbeele ParticleVan Den S. Welburn E. Pays M. Carrington (1997) ArticleTitleThe GPI-phospholipase C of Trypanosoma brucei is nonessential but influences parasitemia in mice J. Cell Biol. 139 103–114 Occurrence Handle10.1083/jcb.139.1.103 Occurrence Handle1:CAS:528:DyaK2sXmsFaqsLo%3D Occurrence Handle9314532