TT2, TT8, and TTG1 synergistically specify the expression of <i>BANYULS</i> and proanthocyanidin biosynthesis in <i>Arabidopsis thaliana</i>

Plant Journal - Tập 39 Số 3 - Trang 366-380 - 2004
Antoine Baudry1, Marc A. Heim2, Bertrand Dubreucq1, Michel Caboche1, Bernd Weißhaar2,3, Loı̈c Lepiniec1
1Seed Biology Laboratory, UMR 204 INRA/INAPG, Jean-Pierre Bourgin Institute, Route de Saint-Cyr, 78026 Versailles Cedex, France, and
2Max-Planck-Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, D-50829 Köln, Germany
3†Current address: Chair of Genome Research, Bielefeld University, D-33594 Bielefeld, Germany.

Tóm tắt

SummaryGenetic analyses have demonstrated that together with TTG1, a WD‐repeat (WDR) protein, TT2 (MYB), and TT8 (bHLH) are necessary for the correct expression of BANYULS (BAN). This gene codes for the core enzyme of proanthocyanidin biosynthesis in Arabidopsis thaliana seed coat. The interplays of TT2, TT8, and their closest MYB/bHLH relatives, with TTG1 and the BAN promoter have been investigated using a combination of genetic and molecular approaches, both in yeast and in planta. The results obtained using glucocorticoid receptor fusion proteins in planta strongly suggest that TT2, TT8, and TTG1 can directly activate BAN expression. Experiments using yeast two‐ and three‐hybrid clearly demonstrated that TT2, TT8, and TTG1 can form a stable ternary complex. Furthermore, although TT2 and TT8 were able to bind to the BAN promoter when simultaneously expressed in yeast, the activity of the complex correlated with the level of TTG1 expression in A. thaliana protoplasts. In addition, transient expression experiments revealed that TTG1 acts mainly through the bHLH partner (i.e. TT8 or related proteins) and that TT2 cannot be replaced by any other related A. thaliana MYB proteins to activate BAN. Finally and consistent with these results, the ectopic expression of TT2 was sufficient to trigger BAN activation in vegetative parts, but only where TTG1 was expressed. Taken together, these results indicate that TT2, TT8, and TTG1 can form a ternary complex directly regulating BAN expression in planta.

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Tài liệu tham khảo

10.1105/tpc.006130

10.1006/meth.1993.1019

10.1016/S1097-2765(00)80022-2

10.1046/j.1365-313X.2003.016010.x

Bechtold N., 1993, In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants, C. R. Acad. Sci. Paris, 316, 1194

10.1105/tpc.12.12.2383

10.1046/j.1365-313X.1998.00031.x

10.1073/pnas.96.22.12929

10.1105/tpc.018796

10.1104/pp.122.2.403

10.1105/tpc.014043

10.1046/j.1365-313X.2003.01844.x

10.1093/emboj/18.19.5370

10.1007/s00438-003-0932-1

10.1128/MCB.15.10.5820

Fairchild C.D., 2000, HFR1 encodes an atypical bHLH protein that acts in phytochrome A signal transduction, Genes Dev., 14, 2377

10.1038/ng0797-277

10.1016/S0076-6879(02)50957-5

10.1101/gad.6.5.864

10.1016/0092-8674(94)90117-1

10.1073/pnas.250379897

10.1016/0092-8674(93)90499-G

10.1023/A:1005921914384

10.1093/molbev/msg088

10.1016/S0079-9920(03)80018-7

10.1093/genetics/144.4.1425

10.1105/tpc.001404

10.1006/dbio.2001.0287

10.1007/BF00331014

Koornneef M., 1981, The complex syndrome of ttg mutant, Arabidopsis Inf. Serv., 18, 45

10.1023/A:1005977103116

10.1093/genetics/151.4.1591

10.1016/S0092-8674(00)81536-6

10.1242/dev.128.9.1539

10.1006/meth.1993.1017

10.1126/science.1465611

10.1126/science.7939683

10.1016/S0031-9422(02)00488-0

10.1046/j.1365-313X.1995.7020333.x

10.1105/tpc.12.10.1863

10.1105/tpc.13.9.2099

10.1105/tpc.004127

10.1016/0092-8674(91)90523-2

10.1093/genetics/156.3.1349

10.1105/tpc.010265

10.1104/pp.010799

10.1126/science.290.5499.2105

10.1101/gad.212702

10.1105/tpc.11.6.1105

10.1006/abio.2000.4672

10.1016/S0968-0004(99)01384-5

10.1023/A:1019850921627

10.1105/tpc.12.9.1619

10.1016/S1369-5266(00)00199-0

10.1093/emboj/17.19.5577

10.1046/j.1365-313X.2003.01939.x

10.1101/gad.11.11.1422

10.1016/S0031-9422(02)00185-1

10.1104/pp.012377

Wagner D., 2002, Arabidopsis: A Laboratory Manual, 296

10.1105/tpc.11.7.1337

10.1017/S0960258500004372

10.1104/pp.126.2.485

10.1126/science.1078540

10.1242/dev.00681

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Plant Journal

Tập 39 Số 3

366-380

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