Tổng hợp các hạt nano silicon phát quang phân tán trong nước và ứng dụng của chúng trong chụp ảnh huỳnh quang sinh học

Springer Science and Business Media LLC - Tập 13 - Trang 405-413 - 2010
Qi Wang1, Hongjun Ni2, Annette Pietzsch3, Franz Hennies3, Yongping Bao4, Yimin Chao1
1Energy Materials Lab, School of Chemistry, University of East Anglia, Norwich, UK
2School of Mechanical Engineering, Nantong University, Nantong, China
3MAX-lab, Lund University, Lund, Sweden
4Biomedical Research Centre, University of East Anglia, Norwich, UK

Tóm tắt

Các hạt nano silicon phân tán trong nước (Si-NPs) rất mong muốn cho các ứng dụng trong các kỹ thuật sinh học. Một phương pháp đơn giản để tổng hợp các hạt như vậy được báo cáo ở đây. Các hạt Si-NPs có khả năng phân tán trong nước và phát quang. Dưới sự kích thích của ánh sáng UV, Si-NPs phát ra ánh sáng đỏ mạnh với đỉnh cực đại ở 606 nm và năng suất lượng tử đạt 6%. Chúng rất ổn định và vẫn giữ được tính ổn định trong vài tuần. Quang phổ hồng ngoại biến đổi Fourier (FTIR) cho thấy một chế độ dao động cắt Si–CH2 rõ ràng. Hơn nữa, các liên kết hóa học trên bề mặt đã được xác nhận bằng quang phổ điện tử năng lượng cao (XPS). Ở các mức năng lượng cốt lõi Si2p và C1s, các thành phần Si–C được quan sát. Đường kính của các hạt Si-NPS được tổng hợp được đo bằng kính hiển vi lực nguyên tử (AFM) khoảng 5 nm. Thêm vào đó, các hạt nano có thể được tế bào nuôi cấy hấp thụ. Hình ảnh huỳnh quang của Si-NPs bên trong tế bào ung thư vú người MCF-7 cho thấy chúng phân bố khắp mô tế bào.

Từ khóa

#nano silicon #phát quang #phân tán trong nước #kỹ thuật sinh học #chụp ảnh huỳnh quang

Tài liệu tham khảo

Alsharif NH, Berger CEM, Varanasi SS, Chao Y, Horrocks BR, Datta HK (2009) Alkyl-capped silicon nanocrystals lack cytotoxicity and have enhanced intracellular accumulation in malignant cells via cholesterol-dependent endocytosis. Small 5:221–228 Arya H, Kaul Z, Wadhwa R, Taira K, Hirano T, Kaul SC (2005) Quantum dots in bio-imaging: revolution by the small. Biochem Biophys Res Commun 329:1173–1177 Belomoin G, Therrien J, Smith A, Rao S, Twesten R, Chaieb S, Nayfeh MH, Wagner L, Mitas L (2002) Observation of a magic discrete family of ultrabright Si nanoparticles. Appl Phys Lett 80:841–843 Bley RA, Kauzlarich SM, Davis JE, Lee HWH (1996) Characterization of silicon nanoparticles prepared from porous silicon. Chem Mater 8:1881–1888 Bychto L, Balaguer M, Pastor E, Chirvony V, Matveeva E (2008) Influence of preparation and storage conditions on photoluminescence of porous silicon powder with embedded Si nanocrystals. J Nanopart Res 10:1241–1249 Chao Y, Krishnamurthy S, Montalti M, Lie LH, Houlton A, Horrocks BR, Kjeldgaard L, Dhanak VR, Hunt MRC, Siller L (2005) Reactions and luminescence in passivated Si nanocrystallites induced by vacuum ultraviolet and soft-X-ray photons. J Appl Phys 98:044316 Chao Y, Houlton A, Horrocks BR, Hunt MRC, Poolton NRJ, Yang J, Šiller L (2006) Optical luminescence from alkyl-passivated Si nanocrystals under vacuum ultraviolet excitation: origin and temperature dependence of the blue and orange emissions. Appl Phys Lett 88:263119 Chao Y, Siller L, Krishnamurthy S, Coxon PR, Bangert U, Gass M, Kjeldgaard L, Patole SN, Lie LH, O’farrell N, Alsop TA, Houlton A, Horrocks BR (2007) Evaporation and deposition of alkyl-capped silicon nanocrystals in ultrahigh vacuum. Nat Nanotechnol 2:486–489 Delerue C, Allan G, Lannoo M (1993) Theoretical aspects of the luminescence of porous silicon. Phys Rev B 48:11024–11036 Denecke R, Vaterlein P, Bassler M, Wassdahl N, Butorin S, Nilsson A, Rubensson JE, Nordgren J, Martensson N, Nyholm R (1999) Beamline I511 at MAX II, capabilities and performance. J Electron Spectrosc Relat Phenom 103:971–977 English DS, Pell LE, Yu Z, Barbara PF, Korgel BA (2002) Size tunable visible luminescence from individual organic monolayer stabilized silicon nanocrystal quantum dots. Nano Lett 2:681–685 Erogbogbo F, Yong KT, Roy I, Xu GX, Prasad PN, Swihart MT (2008) Biocompatible luminescent silicon quantum dots for imaging of cancer cells. Acs Nano 2:873–878 Franchina JG, Lackowski WM, Dermody DL, Crooks RM, Bergbreiter DE, Sirkar K, Russell RJ, Pishko MV (1999) Electrostatic immobilization of glucose oxidase in a weak acid, polyelectrolyte hyperbranched ultrathin film on gold: fabrication, characterization, and enzymatic activity. Anal Chem 71:3133–3139 Froner E, Adamo R, Gaburro Z, Margesin B, Pavesi L, Rigo A, Scarpa M (2006) Luminescence of porous silicon derived nanocrystals dispersed in water: dependence on initial porous silicon oxidation. J Nanopart Res 8:1071–1074 Fujioka K, Hiruoka M, Sato K, Manabe N, Miyasaka R, Hanada S, Hoshino A, Tilley RD, Manome Y, Hirakuri K, Yamamoto K (2008) Luminescent passive-oxidized silicon quantum dots as biological staining labels and their cytotoxicity effects at high concentration. Nanotechnology 19:415102 Hua FJ, Swihart MT, Ruckenstein E (2005) Efficient surface grafting of luminescent silicon quantum dots by photoinitiated hydrosilylation. Langmuir 21:6054–6062 Hua FJ, Erogbogbo F, Swihart MT, Ruckenstein E (2006) Organically capped silicon nanoparticles with blue photoluminescence prepared by hydrosilylation followed by oxidation. Langmuir 22:4363–4370 Li ZF, Ruckenstein E (2004) Water-soluble poly(acrylic acid) grafted luminescent silicon nanoparticles and their use as fluorescent biological staining labels. Nano Lett 4:1463–1467 Li ZF, Kang ET, Neoh KG, Tan KL (1998) Covalent immobilization of glucose oxidase on the surface of polyaniline films graft copolymerized with acrylic acid. Biomaterials 19:45–53 Li QS, Zhang RQ, Niehaus TA, Frauenheim T, Lee ST (2007) Theoretical studies on optical and electronic properties of propionic-acid-terminated silicon quantum dots. J Chem Theor Comput 3:1518–1526 Lie LH, Duerdin M, Tuite EM, Houlton A, Horrocks BR (2002) Preparation and characterisation of luminescent alkylated-silicon quantum dots. J Electroanal Chem 538:183–190 Neiner D, Chiu HW, Kauzlarich SM (2006) Low-temperature solution route to macroscopic amounts of hydrogen terminated silicon nanoparticles. J Am Chem Soc 128:11016–11017 Puniredd SR, Assad O, Haick H (2008) Highly stable organic modification of Si(111) surfaces: towards reacting Si with further functionalities while preserving the desirable chemical properties of full Si–C atop site terminations. J Am Chem Soc 130:9184–9185 Rogozhina E, Belomoin G, Smith A, Abuhassan L, Barry N, Akcakir O, Braun PV, Nayfeh MH (2001) Si–N linkage in ultrabright, ultrasmall Si nanoparticles. Appl Phys Lett 78:3711–3713 Rosso-Vasic M, Spruijt E, Van Lagen B, De Cola L, Zuilhof H (2008) Alkyl-functionalized oxide-free silicon nanoparticles: synthesis and optical properties. Small 4:1835–1841 Rosso-Vasic M, Spruijt E, Popovic Z, Overgaag K, Van Lagen B, Grandidier B, Vanmaekelbergh D, Dominguez-Gutierrez D, de Cola L, Zuilhof H (2009) Amine-terminated silicon nanoparticles: synthesis, optical properties and their use in bioimaging. J Mater Chem 19:5926–5933 Sa’ar A, Reichman Y, Dovrat M, Krapf D, Jeduejewski J, Balberg I (2005) Resonant coupling between surface vibrations and electronic states in silicon nanocrystals at the strong confinement regime. Nano Lett 5:2443–2447 Sato S, Swihart MT (2006) Propionic-acid-terminated silicon nanoparticles: synthesis and optical characterization. Chem Mater 18:4083–4088 Seotsanyana-Mokhosi I, Kuznetsova N, Nyokong T (2001) Photochemical studies of tetra-2,3-pyridinoporphyrazines. J Photochem Photobiol A Chem 140:215–222 Shiohara A, Hanada S, Prabakar S, Fujioka K, Lim TH, Yamamoto K, Northcote PT, Tilley RD (2010) Chemical reactions on surface molecules attached to silicon quantum dots. J Am Chem Soc 132:248–253 Tilley RD, Yamamoto K (2006) The microemulsion synthesis of hydrophobic and hydrophilic silicon nanocrystals. Adv Mater 18:2053–2056 Warner JH, Hoshino A, Yamamoto K, Tilley RD (2005) Water-soluble photoluminescent silicon quantum dots. Angew Chem Int Ed 44:4550–4554 Williams ATR, Winfield SA, Miller JN (1983) Relative fluorescence quantum yields using a computer-controlled luminescence spectrometer. Analyst 108:1067–1071 Wolkin MV, Jorne J, Fauchet PM, Allan G, Delerue C (1999) Electronic states and luminescence in porous silicon quantum dots: the role of oxygen. Phys Rev Lett 82:197–200 Wu K, Song L, Hu Y, Lu HD, Kandola BK, Kandare E (2009) Synthesis and characterization of a functional polyhedral oligomeric silsesquioxane and its flame retardancy in epoxy resin. Prog Org Coat 65:490–497 Yu ZR, Aceves-Mijares M, Cabrera MAI (2006) Single electron charging and transport in silicon rich oxide. Nanotechnology 17:3962–3967 Zhang XM, Neiner D, Wang SZ, Louie AY, Kauzlarich SM (2007) A new solution route to hydrogen-terminated silicon nanoparticles: synthesis, functionalization and water stability. Nanotechnology 18:095601 Zhu XP, Yukawa T, Kishi T, Hirai M, Suematsu H, Jiang WH, Yatsui K (2005) Synthesis of light-emitting silicon nanoparticles by intense pulsed ion-beam evaporation. J Nanopart Res 7:669–673