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Tổng hợp các hạt nano silicon phát quang phân tán trong nước và ứng dụng của chúng trong chụp ảnh huỳnh quang sinh học
Tóm tắt
Các hạt nano silicon phân tán trong nước (Si-NPs) rất mong muốn cho các ứng dụng trong các kỹ thuật sinh học. Một phương pháp đơn giản để tổng hợp các hạt như vậy được báo cáo ở đây. Các hạt Si-NPs có khả năng phân tán trong nước và phát quang. Dưới sự kích thích của ánh sáng UV, Si-NPs phát ra ánh sáng đỏ mạnh với đỉnh cực đại ở 606 nm và năng suất lượng tử đạt 6%. Chúng rất ổn định và vẫn giữ được tính ổn định trong vài tuần. Quang phổ hồng ngoại biến đổi Fourier (FTIR) cho thấy một chế độ dao động cắt Si–CH2 rõ ràng. Hơn nữa, các liên kết hóa học trên bề mặt đã được xác nhận bằng quang phổ điện tử năng lượng cao (XPS). Ở các mức năng lượng cốt lõi Si2p và C1s, các thành phần Si–C được quan sát. Đường kính của các hạt Si-NPS được tổng hợp được đo bằng kính hiển vi lực nguyên tử (AFM) khoảng 5 nm. Thêm vào đó, các hạt nano có thể được tế bào nuôi cấy hấp thụ. Hình ảnh huỳnh quang của Si-NPs bên trong tế bào ung thư vú người MCF-7 cho thấy chúng phân bố khắp mô tế bào.
Từ khóa
#nano silicon #phát quang #phân tán trong nước #kỹ thuật sinh học #chụp ảnh huỳnh quangTài liệu tham khảo
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