Tổng hợp nanoparticle bạc và vàng từ dịch chiết lá Jasminum nervosum và hoạt động diệt ấu trùng của chúng đối với véc tơ giun chỉ và virut arbo Culex quinquefasciatus Say (Diptera: Culicidae)

Springer Science and Business Media LLC - Tập 22 - Trang 17753-17768 - 2015
H. Lallawmawma1, Gnanasekar Sathishkumar2, Subburayan Sarathbabu1, Souvik Ghatak1, Sivaperumal Sivaramakrishnan2, Guruswami Gurusubramanian3, Nachimuthu Senthil Kumar1
1Department of Biotechnology, Mizoram University, Aizawl, India
2Department of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappalli, India
3Department of Zoology, Mizoram University, Aizawl, India

Tóm tắt

Nanoparticle bạc và vàng từ Jasminum nervosum L. có các tính chất quang học độc đáo như dải hấp thụ rộng trong vùng nhìn thấy của quang phổ điện từ. Việc xác định các particle này bằng cách sử dụng phổ kế UV, quang phổ hồng ngoại biến đổi Fourier, nhiễu xạ tia X và kính hiển vi điện tử truyền dẫn đã xác nhận rằng các hạt là bạc (AgNPs) và vàng (AuNPs) có kích thước từ 4–22 nm và 2–20 nm với kích thước trung bình lần lượt là 9.4 nm và 10 nm. AgNPs và AuNPs từ J. nervosum có hoạt tính diệt ấu trùng cao đối với véc tơ giun chỉ và virut arbo, Culex quinquefasciatus, hơn so với dịch chiết lá nước. Nồng độ gây chết (LC50 và LC95) được quan sát đối với ấu trùng tuổi ba là 57.40 và 144.36 μg/ml cho AgNPs và 82.62 và 254.68 μg/ml cho AuNPs sau 24 giờ điều trị, tương ứng. Thời gian chết để tiêu diệt 50% ấu trùng C. quinquefasciatus là 2.24 và 4.51 giờ ở nồng độ 150 μg/ml của AgNPs và AuNPs, trong khi đối với dịch chiết lá nước của J. nervosum thì là 9.44 giờ ở nồng độ 500 μg/ml (F 2,14 = 397.51, P < 0.0001). Biểu đồ phân tích thành phần chính trình bày sự phân cụm khác nhau giữa dịch chiết lá nước, AgNP và AuNPs liên quan đến liều lượng gây chết và thời gian gây chết. Kết luận từ các phát hiện hiện tại cho thấy các AgNPs và AuNPs được tổng hợp sinh học từ dịch chiết lá nước của J. nervosum có thể là một loại thuốc trừ sâu nanobi an toàn hơn cho môi trường và có hiệu quả diệt ấu trùng tiềm năng đối với ấu trùng C. quinquefasciatus, có thể được sử dụng để phòng ngừa nhiều bệnh tật đáng sợ.

Từ khóa

#nanoparticle bạc #nanoparticle vàng #Jasminum nervosum #hoạt động diệt ấu trùng #Culex quinquefasciatus #thuốc trừ sâu sinh học

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