Synthesis of multi-responsive poly(NIPA-co-DMAEMA)-PBA hydrogel nanoparticles in aqueous solution for application as glucose-sensitive insulin-releasing nanoparticles
Tóm tắt
This study aimed to present an innovative method for synthesizing pH-thermo-glucose responsive poly(NIPA-co-DMAEMA)-PBA hydrogel nanoparticles via single-step aqueous free radical polymerization. The synthesis process involved free radical polymerization in an aqueous solution, and the resulting nanoparticles were characterized for their physical and chemical properties by 1H NMR, Dynamic Light Scattering (DLS) and Scanning Electron Microscopy (SEM). Insulin-loaded poly(NIPA-co-DMAEMA)-PBA hydrogel nanoparticles were prepared and evaluated for their insulin capture and release properties at different pH and temperature, in addition to different glucose concentrations, with the release profile of insulin quantitatively evaluated using the Bradford method. 1H NMR results confirmed successful PBA incorporation, and DLS outcomes consistently indicated a transition to a more hydrophobic state above the Lower Critical Solution Temperature (LCST) of NIPA and DMAEMA. While pH responsiveness exhibited variation, insulin release generally increased with rising pH from acidic to neutral conditions, aligning with the anticipated augmentation of anionic PBA moieties and increased hydrogel hydrophilicity. Increased insulin release in the presence of glucose, particularly for formulations with the lowest mol % PBA, along with a slight increase for the highest mol % PBA formulation when increasing glucose from 1 to 4 mg/mL, supported the potential of this approach for nanoparticle synthesis tailored for glucose-responsive insulin release. This work successfully demonstrates a novel method for synthesizing responsive hydrogel nanoparticles and underscores their potential for controlled insulin release in response to glucose concentrations. The observed pH-dependent insulin release patterns and the influence of PBA content on responsiveness highlight the versatility and promise of this nanoparticle synthesis approach for applications in glucose-responsive drug delivery systems. Poly(NIPA) nanoparticles containing PBA moieties are normally synthesized in two or more steps in the presence of organic solvents. Here we propose a new method for the synthesis of multiresponsive hydrogel poly(NIPA-co-DMAEMA)-PBA nanoparticles in aqueous medium in a single reaction to provide a fast and effective strategy for the production of glucose-responsive multi-systems in aqueous media from free radical polymerization
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Tài liệu tham khảo
Cannon MJ, Ng BP, Lloyd K, Reynolds J, Ely EK. J Diabetes Res.2022;1–9.
Gholami M, Jackson NJ, Chung UYR, Duru OK, Shedd K, Soetenga S, Loeb T, Elashoff D, Hamilton AB, Mangione CM, Slusser W, Moin T. BMC Public Health. 2021;21:1.
Saeedi P, Salpea P, Karuranga S, Petersohn I, Malanda B, Gregg EW, Unwin N, Wild SH, Williams R. R Diabetes Res Clin Pract. 2020;162:108086.
Siegel KR, Ali MK, Zhou X, Ng BP, Jawanda S, Proia K, Zhang X, Gregg EW, Albright AL, Zhang P. Diabetes Care. 2020;43(7):1557–92.
Parveen R, Sehar N, Bajpai R, Agarwal NB. Diabetes Res Clin Pract. 2020;166:108295.
Lavrador P, Esteves MR, Gaspar VM, Mano JF. Adv Funct Mater. 2021;31:2005941.
Chafran L, Carfagno A, Altalhi A, Bishop B. Polymers. 2022;14:4755.
Carfagno A, Lin S-C, Chafran L, Akhrymuk I, Callahan V, Po M, Zhu Y, Altalhi A, Durkin DP, Russo P, Vliet KA, Webb-Robertson B, Kehn-Hall K, Bishop B. Proteomics 2023;23:e2200237.
Alvarez-Bautista A, Duarte CMM, Mendizábal E, Katime I. Des Monomers Polym. 2016;19:319–29.
Herrmann A, Haag R, Schedler U. Adv Healthc Mater. 2021;10:2100062.
Rosiak JM, Yoshii F. Nucl Instrum Methods Phys Res B NUCL INSTRUM METH B. 1999;151(1–4):56–64.
Zhang Z, Wang S, Waterhouse G, Zhang Q, Li L. J Appl Polym Sci. 2020;137:48391.
Jiménez RR, Carfagno A, Linhoff L, Gratwick B, Woodhams D, Chafran LS, Bletz MC, Bishop B, Muletz-Wolz CR. Appl Environ Microbiol. 2022;88(8):e01818-01821.
Safavi-Mirmahalleh SA, Salami-Kalajahi M, Roghani-Mamaqani H. Environ Sci Pollut Res. 2020;27:28091–103.
Wang C, Lin B, Zhu H, Bi F, Xiao S, Wang L, Gai G, Zhao L. Molecules. 2019;24:1089.
Najmeddine AA, Saeed M, Beadham IG, ElShaer A. PharmaNutrition2021;18:100280.
Wu JZ, Yang Y, Li S, Shi A. Int J Nanomed. 2019;14:8059–72.
Ma Q, Bian L, Zhao X, Tian X, Yin H, Wang Y, Shi A, Wu J. Mater Today Bio. 2022;13:100181.
Matsumoto A, Ikeda S, Harada A, Kataoka K. Biomacromol. 2003;4(5):1410–6.
Matsumoto A, Yoshida R, Kataoka K. Biomacromol. 2004;5(3):1038–45.
Zhang Y, Guan Y, Zhou S. Biomacromol. 2006;7(11):3196–201.
Tang Z, Guan Y, Zhang Y. Polym Chem. 2014;5:1782–90.
Elshaarani T, Yu H, Wang L, Lin L, Wang N, Naveed KR, Zhang L, Han Y, Fahad S, Ni Z. Eur Polym J. 2020;125:109505.
Wu JZ, Williams GR, Li HY, Wang D, Wu H, Li SD, Zhu LM. Int J Nanomed. 2017;12:4037–57.
Farooqi ZH, Khan A, Siddiq M. Polym Int. 2011;60:1481–6.
Shi Q, Jackowski G. In: Hames BD, editor. Gel electrophoresis of proteins: a practical Approach. 1sted. ed. Oxford: Publisher:Oxford University; 1998. pp. 13–34.
Arcos-Hernandez M, Naidjonoka P, Butler SJ, Nylander T, Stålbrand H, Jannasch P. Biomacromol. 2021;22(6):2338–51.
Monaco A, Drain B, Becer CR. Polym Chem. 2021;12:5229–38.
Cao J, Liu S, Chen Y, Shi L, Zhang Z. Polym Chem. 2014;5(17):5029–36.
Sasaki S. J Phys Chem. 2016;120(1):184–92.
Abdelaty MSA. J Polym Res. 2021;28:213.
Xu X, Liu Y, Fu W, Yao M, Ding Z, Xuan J, Li D, Wang S, Xia Y, Cao M. Polymers. 2020;12:580.
Sagle LB, Zhang Y, Litosh VA, Chen X, Cho Y, Cremer PS. J Am Chem Soc. 2009;131(26):9304–10.
Dong Z, Wei H, Mao J, Wang D, Yang M, Bo S, Ji X. Polymer. 2012;53(10):2074–84.
Du H, Wickramasinghe R, Qian X. J Phys Chem. 2010;114(49):16594–604.
Ma G–H, Nagai M, Omi S. J Appl Polym Sci. 2001;79:2408–24.
Roointan A, Farzanfar J, Mohammadi-Samani S, Behzad-Behbahani A, Farjadian F. Smart pH responsive drug delivery system based on poly(HEMA-co-DMAEMA) nanohydrogel. Int J Pharm. 2018;552(1–2):301–11. https://doi.org/10.1016/j.ijpharm.2018.10.001.
Orakdogen N. J Polym Res. 2012;19:9914.
Wang Y, Zhang X, Han Y, Cheng C, Li C. Carbohydr Polym. 2012;89(1):124–31.
Zheng C, Guo Q, Wu Z, Sun L, Zhang Z, Li C, Zhang X. Eur J Pharm Sci. 2013;49(4):474–82.
Shiomori K, Ivanov AE, Galaev IY, Kawano Y, Mattiasson B. Macromol Chem Phys. 2004;205:27–34.
Burdukova E, Li H, Ishida N, O’Shea JP, Franks GV. J Colloid Interface Sci. 2010;342(2):586–92.
Kardos A, Gilányi T, Varga I. J Colloid Interface Sci. 2019;557(1):793–806.
Guo H, Li H, Gao J, Zhao G, Ling L, Wang B, Guo Q, Gu Y, Li C. Polym Chem. 2016;7:3189–99.
Wu J, Bremner DH, Li H, Sun X, Zhu L. Synthesis and evaluation of temperature- and glucose-sensitive nanoparticles based on phenylboronic acid and N-vinylcaprolactam for insulin delivery. Mater Sci Eng: C. 2016;69:1026–35. https://doi.org/10.1016/j.msec.2016.07.078.
Hei M, Wu H, Fu Y, Xu Y, Zhu W. J Drug Deliv Sci Technol. 2019;51:320–6.
Dey GR, Saha A. ACS Biomater Sci Eng. 2021;7(9):4645–58.
Sun L, Zhang X, Zheng C, Wu Z, Xia X, Li C. RSC Adv. 2012;2(26):9904–13.