Sustained high level transgene expression in mammalian cells mediated by the optimized piggyBac transposon system

Ellis, 2005, Retrovirus silencing and vector design: relevance to normal and cancer stem cells?, Curr Gene Ther, 5, 367, 10.2174/1566523054546233 Hotta, 2008, Retroviral vector silencing during iPS cell induction: an epigenetic beacon that signals distinct pluripotent states, J Cell Biochem, 105, 940, 10.1002/jcb.21912 Fisher, 2011, Chromatin states in pluripotent, differentiated, and reprogrammed cells, Curr Opin Genet Dev, 21, 140, 10.1016/j.gde.2011.01.015 Ng, 2011, The transcriptional and signalling networks of pluripotency, Nat Cell Biol, 13, 490, 10.1038/ncb0511-490 Orkin, 2011, Chromatin connections to pluripotency and cellular reprogramming, Cell, 145, 835, 10.1016/j.cell.2011.05.019 Young, 2011, Control of the embryonic stem cell state, Cell, 144, 940, 10.1016/j.cell.2011.01.032 Beisel, 2011, Silencing chromatin: comparing modes and mechanisms, Nat Rev Genet, 12, 123, 10.1038/nrg2932 Stewart, 2008, Silencing of the expression of pluripotent driven-reporter genes stably transfected into human pluripotent cells, Regen Med, 3, 505, 10.2217/17460751.3.4.505 Wen, 2014, Characterization of constitutive promoters for piggyBac transposon-mediated stable transgene expression in mesenchymal stem cells (MSCs), PloS One, 9, e94397, 10.1371/journal.pone.0094397 Kim, 2011, Size matters: versatile use of PiggyBac transposons as a genetic manipulation tool, Mol Cell Biochem, 354, 301, 10.1007/s11010-011-0832-3 Di Matteo, 2012, PiggyBac toolbox, Methods Mol Biol, 859, 241, 10.1007/978-1-61779-603-6_14 Ding, 2005, Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice, Cell, 122, 473, 10.1016/j.cell.2005.07.013 Saridey, 2009, PiggyBac transposon-based inducible gene expression in vivo after somatic cell gene transfer, Mol Ther, 17, 2115, 10.1038/mt.2009.234 Woltjen, 2009, piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells, Nature, 458, 766, 10.1038/nature07863 Yusa, 2009, Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon, Nat Methods, 6, 363, 10.1038/nmeth.1323 Fraser, 1983, Acquisition of host cell DNA sequences by baculoviruses: relationship between host DNA insertions and FP mutants of Autographa californica and Galleria mellonella nuclear polyhedrosis viruses, J virol, 47, 287, 10.1128/JVI.47.2.287-300.1983 Wu, 2006, piggyBac is a flexible and highly active transposon as compared to sleeping beauty, Tol2, and Mos1 in mammalian cells, Proc Natl Acad Sci U S A, 103, 15008, 10.1073/pnas.0606979103 Wilson, 2007, PiggyBac transposon-mediated gene transfer in human cells, Mol Ther, 15, 139, 10.1038/sj.mt.6300028 Huang, 2012, Conditionally immortalized mouse embryonic fibroblasts retain proliferative activity without compromising multipotent differentiation potential, PloS One, 7, e32428, 10.1371/journal.pone.0032428 Wang, 2014, The piggyBac transposon-mediated expression of SV40 T antigen efficiently immortalizes mouse embryonic fibroblasts (MEFs), PloS One, 9, e97316, 10.1371/journal.pone.0097316 Wu, 2014, Overexpression of Ad5 precursor terminal protein accelerates recombinant adenovirus packaging and amplification in HEK-293 packaging cells, Gene Ther, 21, 629, 10.1038/gt.2014.40 Luo, 2008, Osteogenic BMPs promote tumor growth of human osteosarcomas that harbor differentiation defects, Lab Invest, 88, 1264, 10.1038/labinvest.2008.98 Cheng, 2003, Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs), J Bone Joint Surg Am, 85-A, 1544, 10.2106/00004623-200308000-00017 Haydon, 2002, Nuclear receptor agonists as potential differentiation therapy agents for human osteosarcoma, Clin Cancer Res, 8, 1288 Peng, 2003, Transcriptional characterization of bone morphogenetic proteins (BMPs)-mediated osteogenic signaling, J Cell Biochem, 90, 1149, 10.1002/jcb.10744 Tang, 2009, BMP9-induced osteogenic differentiation of mesenchymal progenitors requires functional canonical Wnt/beta-catenin signaling, J Cell Mol Med, 13, 2448, 10.1111/j.1582-4934.2008.00569.x He, 1998, A simplified system for generating recombinant adenoviruses, Proc Natl Acad Sci U S A, 95, 2509, 10.1073/pnas.95.5.2509 Luo, 2007, A protocol for rapid generation of recombinant adenoviruses using the AdEasy system, Nat Protoc, 2, 1236, 10.1038/nprot.2007.135 Kang, 2004, Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery, Gene Ther, 11, 1312, 10.1038/sj.gt.3302298 He, 2011, Tetrandrine inhibits Wnt/beta-catenin signaling and suppresses tumor growth of human colorectal cancer, Mol Pharmacol, 79, 211, 10.1124/mol.110.068668 Hu, 2013, BMP9-regulated angiogenic signaling plays an important role in the osteogenic differentiation of mesenchymal progenitor cells, J cell Sci, 126, 532, 10.1242/jcs.114231 Luther, 2013, IGFBP5 domains exert distinct inhibitory effects on the tumorigenicity and metastasis of human osteosarcoma, Cancer Lett, 336, 222, 10.1016/j.canlet.2013.05.002 Rastegar, 2010, Lysophosphatidic acid acyltransferase beta (LPAATbeta) promotes the tumor growth of human osteosarcoma, PloS One, 5, e14182, 10.1371/journal.pone.0014182 Bi, 2014, Functional characteristics of reversibly immortalized hepatic progenitor cells derived from mouse embryonic liver, Cell Physiol Biochem, 34, 1318, 10.1159/000366340 Wang, 2014, Bone morphogenetic protein-9 (BMP9) effectively induces osteo/odontoblastic differentiation of the reversibly immortalized stem cells of dental apical papilla, Stem Cells Dev, 23, 1405, 10.1089/scd.2013.0580 Wang, 2014, Adenovirus-mediated efficient gene transfer into cultured three-dimensional organoids, PloS One, 9, e93608, 10.1371/journal.pone.0093608 Li, 2014, Targeting BMP9-promoted human osteosarcoma growth by inactivation of notch signaling, Curr Cancer Drug Targets, 14, 274, 10.2174/1568009614666140305105805 Zhao, 2014, Adenovirus-mediated gene transfer in mesenchymal stem cells can be significantly enhanced by the cationic polymer polybrene, PloS One, 9, e92908, 10.1371/journal.pone.0092908 Si, 2006, CCN1/Cyr61 is regulated by the canonical Wnt signal and plays an important role in Wnt3A-induced osteoblast differentiation of mesenchymal stem cells, Mol Cell Biol, 26, 2955, 10.1128/MCB.26.8.2955-2964.2006 Sharff, 2009, Hey1 basic helix-loop-helix protein plays an important role in mediating BMP9-induced osteogenic differentiation of mesenchymal progenitor cells, J biological Chem, 284, 649, 10.1074/jbc.M806389200 Kang, 2009, A comprehensive analysis of the dual roles of BMPs in regulating adipogenic and osteogenic differentiation of mesenchymal progenitor cells, Stem Cells Dev, 18, 545, 10.1089/scd.2008.0130 Bi, 2009, Wnt antagonist SFRP3 inhibits the differentiation of mouse hepatic progenitor cells, J Cell Biochem, 108, 295, 10.1002/jcb.22254 Zhu, 2009, Activation of RXR and RAR signaling promotes myogenic differentiation of myoblastic C2C12 cells, Differ Res biological Divers, 78, 195, 10.1016/j.diff.2009.06.001 Huang, 2009, Retinoic acid signalling induces the differentiation of mouse fetal liver-derived hepatic progenitor cells, Liver Int, 29, 1569, 10.1111/j.1478-3231.2009.02111.x Huang, 2012, Growth hormone synergizes with BMP9 in osteogenic differentiation by activating the JAK/STAT/IGF1 pathway in murine multilineage cells, J Bone Min Res, 27, 1566, 10.1002/jbmr.1622 Yang, 2012, Conditional immortalization establishes a repertoire of mouse melanocyte progenitors with distinct melanogenic differentiation potential, J Invest Dermatol, 132, 2479, 10.1038/jid.2012.145 Li, 2013, Establishment and characterization of the reversibly immortalized mouse fetal heart progenitors, Int J Med Sci, 10, 1035, 10.7150/ijms.6639 Lamplot, 2014, Reversibly immortalized mouse articular chondrocytes acquire long-term proliferative capability while retaining chondrogenic phenotype, Cell Transplant Liu, 2013, Cross-talk between EGF and BMP9 signalling pathways regulates the osteogenic differentiation of mesenchymal stem cells, J Cell Mol Med, 17, 1160, 10.1111/jcmm.12097 Deng, 2014, A simplified and versatile system for the simultaneous expression of multiple siRNAs in mammalian cells using Gibson DNA assembly, PloS One, 9, e113064, 10.1371/journal.pone.0113064 Zhang, 2013, Modulation of beta-catenin signaling by the inhibitors of MAP kinase, tyrosine kinase, and PI3-kinase pathways, Int J Med Sci, 10, 1888, 10.7150/ijms.6019 Wang, 2014, Decellularized liver scaffolds effectively support the proliferation and differentiation of mouse fetal hepatic progenitors, J Biomed Mater Res, 102, 1017, 10.1002/jbm.a.34764 Wang, 2013, Noggin resistance contributes to the potent osteogenic capability of BMP9 in mesenchymal stem cells, J Orthop Res, 31, 1796, 10.1002/jor.22427 Shui, 2014, Characterization of chondrocyte scaffold carriers for cell-based gene therapy in articular cartilage repair, J Biomed Mater Res, 101, 3542, 10.1002/jbm.a.34661 He, 2010, Synergistic antitumor effect of the activated PPARgamma and retinoid receptors on human osteosarcoma, Clin Cancer Res, 16, 2235, 10.1158/1078-0432.CCR-09-2499 Chen, 2013, The E-F hand calcium-binding protein S100A4 regulates the proliferation, survival and differentiation potential of human osteosarcoma cells, Cell Physiol Biochem, 32, 1083, 10.1159/000354508 Zhang, 2014, Bone morphogenetic protein 2 inhibits the proliferation and growth of human colorectal cancer cells, Oncol Reports, 32, 1013, 10.3892/or.2014.3308 Breyer, 2001, Adenoviral vector-mediated gene transfer for human gene therapy, Curr Gene Ther, 1, 149, 10.2174/1566523013348689 McConnell, 2004, Biology of adenovirus and its use as a vector for gene therapy, Hum Gene Ther, 15, 1022, 10.1089/hum.2004.15.1022 Hoeben, 2013, Adenovirus DNA replication, Cold Spring Harb Perspect Biol, 5, a013003, 10.1101/cshperspect.a013003 Li, 2013, piggyBac transposase tools for genome engineering, Proc Natl Acad Sci U S A, 110, E2279, 10.1073/pnas.1305987110 Yusa, 2011, A hyperactive piggyBac transposase for mammalian applications, Proc Natl Acad Sci U S A, 108, 1531, 10.1073/pnas.1008322108