Surface Functionalized Nanofibrillar Cellulose (NFC) Film as a Platform for Immunoassays and Diagnostics

Biointerphases - Tập 7 Số 1 - 2012
Hannes Orelma1, Ilari Filpponen1, Leena‐Sisko Johansson1, Monika Österberg1, Orlando J. Rojas2, Janne Laine1
1Aalto University 1 Department of Forest Products Technology, School of Chemical Technology, , Espoo, 00076, Finland
2North Carolina State University 2 Department of Forest Biomaterials, , Raleigh, NC 27695, USA

Tóm tắt

We introduce a new method to modify films of nanofibrillated cellulose (NFC) to produce non-porous, water-resistant substrates for diagnostics. First, water resistant NFC films were prepared from mechanically disintegrated NFC hydrogel, and then their surfaces were carboxylated via TEMPO-mediated oxidation. Next, the topologically functionalized film was activated via EDS/NHS chemistry, and its reactivity verified with bovine serum albumin and antihuman IgG. The surface carboxylation, EDC/NHS activation and the protein attachment were confirmed using quartz crystal microbalance with dissipation, contact angle measurements, conductometric titrations, X-ray photoelectron spectroscopy and fluorescence microscopy. The surface morphology of the prepared films was investigated using confocal laser scanning microscopy and atomic force microscopy. Finally, we demonstrate that antihuman IgG can be immobilized on the activated NFC surface using commercial piezoelectric inkjet printing.

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Tài liệu tham khảo

2008, Anal Chem, 80, 6928, 10.1021/ac800604v

2008, Bioresources, 3, 1315

2007, Chem Phys Lett, 435, 342, 10.1016/j.cplett.2006.12.068

2005, Langmuir, 21, 5966, 10.1021/la0503294

1995, Carbohydr Res, 269, 89, 10.1016/0008-6215(94)00343-E

2009, Soft Matter, 5, 4866, 10.1039/b909878j

2010, Bioactive paper through inkjet printing, J Adhes Sci Technol, 24, 661, 10.1163/016942409X12561252292387

2007, Cellulose, 14, 257, 10.1007/s10570-006-9104-x

2009, Biomacromolecules, 10, 162, 10.1021/bm801065u

2006, Cellulose, 13, 679, 10.1007/s10570-006-9075-y

1987, Clin Chem, 33, 1070

2008, Bioconjugate techniques

2010, Angew Chem, 122, 7836, 10.1002/ange.201003848

1998, Macromolecules, 31, 6297, 10.1021/ma971032i

1998, Langmuir, 14, 729, 10.1021/la970815u

2009, Anal Chem, 81, 5474, 10.1021/ac900660p

1998, Cellulose, 5, 153, 10.1023/A:1009208603673

1992, Phys Rev B, 46, 7808, 10.1103/PhysRevB.46.7808

2004, Surf Interface Anal, 36, 1018, 10.1002/sia.1827

2011, Soft Matter, 7, 10917, 10.1039/c1sm06073b

2011, Soft Matter, 7, 3467, 10.1039/c0sm01355b

2005, Clin Chem, 51, 2415, 10.1373/clinchem.2005.051532

2008, J Biochem Biophys Methods, 70, 1180, 10.1016/j.jbbm.2007.05.003

1998, J Colloid Interface Sci, 202, 507, 10.1006/jcis.1998.5513

Wong, 2005, Drugs of abuse, 71, 10.1007/978-1-59259-951-6

1998, Formation of adsorbed protein layers, J Colloid Interface Sci, 207, 186, 10.1006/jcis.1998.5763

2009, Adv Mater, 21, 1595, 10.1002/adma.200803174

2008, Colloids Surf B, 61, 1, 10.1016/j.colsurfb.2007.09.029

1996, Macromol Symp, 103, 5, 10.1002/masy.19961030104

2011, Biomacromolecules, 12, 4311, 10.1021/bm201236a

2012, Biomacromolecules, 13, 1051, 10.1021/bm201771m

2007, Biomacromolecules, 8, 1934, 10.1021/bm061215p

2009, Trends Anal Chem, 28, 925, 10.1016/j.trac.2009.05.005

1995, Rev Sci Instrum, 66, 3924, 10.1063/1.1145396

2001, J Pharmacol Toxicol Methods, 45, 247, 10.1016/S1056-8719(01)00157-5

1999, Drug Discov Today, 4, 411, 10.1016/S1359-6446(99)01388-4

2004, Biomacromolecules, 5, 1983, 10.1021/bm0497769

2007, Biomacromolecules, 8, 2485, 10.1021/bm0703970

2006, Biomacromolecules, 7, 1687, 10.1021/bm060154s

2010, Cellulose, 17, 459, 10.1007/s10570-010-9405-y

2010, Cellulose, 17, 835, 10.1007/s10570-010-9424-8

1985, J Immunol Methods, 81, 161, 10.1016/0022-1759(85)90132-2

1986, Anal Biochem, 156, 220, 10.1016/0003-2697(86)90176-4

1998, J Phys Chem B, 102, 8100, 10.1021/jp981239t

2009, Cellulose, 16, 75, 10.1007/s10570-008-9244-2

2011, Cellulose, 18, 393, 10.1007/s10570-010-9478-7

2008, J Biomater Sci Polym Ed, 19, 725, 10.1163/156856208784522092

2006, Nature, 442, 412, 10.1038/nature05064

2004, J Mater Sci, 39, 1635, 10.1023/B:JMSC.0000016162.43897.0a

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Tập 7 Số 1

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