Supercoils in plant DNA: Nucleoid sedimentation studies

Journal of Cell Science - Tập 89 Số 2 - Trang 243-252 - 1988
Roberta Meschini1, Jordanka Zlatanova1, Anna Vassileva1, Maria G. Ivanchenko1, Chavdar Krachmarov1, Dobri D. Genchev1
1Institute of Genetics, Bulgarian Academy of Sciences, Sofia

Tóm tắt

ABSTRACT Plant nuclei have been studied with respect to the three-dimensional structure of DNA. Nucleoids derived from nuclei by non-ionic detergent and high salt treatment were analysed by sedimentation in a series of sucrose gradients containing increasing amounts of the intercalating agent ethidium bromide. In addition the nucleoid sedimentation behaviour was investigated following gamma irradiation. The results show that plant DNA is supercoiled, as is the DNA from the other eukaryotes studied, and contains approximately the same concentration of superhelical turns but probably relatively fewer DNA superhelical loops. The plant nuclear populations in all cases studied give rise to two distinct nucleoid bands. These have been characterized by electron microscopy and by their DNA and protein content. The possible origin of the two bands is discussed.

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