Xiaodong Yan1, G Charette, EE Delvin
1Genetics Unit, Shriners Hospital, Montréal, Que., Canada.
Tóm tắt
We have studied the submitochondrial localization of guinea-pig kidney 25-hydroxycholecalciferol 1α-hydroxylase. Treatment of the mitochondrial-enriched fraction with recrystallized digitonin produced mitoplasts bordered by a single membrane and with intact matrix. They contained nearly 90% of the 25-hydroxycholecalciferol 1α-hydroxylase activity and nearly 100% of the cytochrome-c: oxygen oxidoreductase. Amine: oxygen oxidoreductase activity remained mainly in the outer membrane fraction. These data show that 25-hydroxycholecalciferol 1α-hydroxylase has a distribution similar to that of steroid hydroxylases.
