Subchronic organismal toxicity, cytotoxicity, genotoxicity, and feeding response of pacific oyster (<i>Crassostrea gigas</i>) to lindane (γ‐HCH) exposure under experimental conditions

Environmental Toxicology and Chemistry - Tập 26 Số 10 - Trang 2192-2197 - 2007
Gerardo Anguiano1, Raúl Llera‐Herrera1, Emilio Rojas2, Celia Vázquez‐Boucard1
1Department of Environmental Management and Conservation, Toxicogenomic and Reproduction of Aquatic Organism Laboratory (CIBNOR), P.O. 128, La Paz, Baja California Sur, México
2Department of Genomic and Environmental Toxicology Medicine. Toxicogenomic Laboratory. IIB-UNAM. Ciudad de México, México

Tóm tắt

AbstractThis study evaluated organismal toxicity, cytotoxicity, and genotoxicity and the filtration rate in response to different concentrations of subchronic lindane (gamma‐hexachlorocyclohexane [γ‐HCH]), exposure (12 d) in adult Pacific oysters Crassostrea gigas. Oysters were exposed in vivo in laboratory aquaria to 10 different concentrations (0.0–10.0 mg/L) of γ‐HCH. The median lethal concentration (LC50) after 12 d was calculated as 2.22 mg/L. Cytotoxic effects were observed in hemocytes, where the mean cell viability was significantly decreased at 1.0 mg/L of γ‐HCH after 12 d. Genotoxicity of γ‐HCH measured by single cell gel electrophoresis assay, in hemocytes was evident at 0.7 mg/L of γ‐HCH after 12 d. After 4 h of exposure to γ‐HCH, filtration rates were reduced compared with controls to 65.8 and 38.2% at concentrations of 0.3 and 0.7 mg/L, respectively, and after 11 d of exposure, filtration rates were reduced to 60.4 and 30.9% at concentrations of 0.1 mg/L and higher. These results show the subchronic effects of γ‐HCH at different concentrations and effect sensitivities are categorized as filtration rate < genotoxicity < cytotoxicity < mortality. The relevance of integral toxicity evaluation, considering different endpoints from molecular, cellular, and individual levels is discussed.

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Thông tin xuất bản

Environmental Toxicology and Chemistry

Tập 26 Số 10

2192-2197

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