Subchronic organismal toxicity, cytotoxicity, genotoxicity, and feeding response of pacific oyster (<i>Crassostrea gigas</i>) to lindane (γ‐HCH) exposure under experimental conditions

Environmental Toxicology and Chemistry - Tập 26 Số 10 - Trang 2192-2197 - 2007
Gerardo Anguiano1, Raúl Llera‐Herrera1, Emilio Rojas2, Celia Vázquez‐Boucard1
1Department of Environmental Management and Conservation, Toxicogenomic and Reproduction of Aquatic Organism Laboratory (CIBNOR), P.O. 128, La Paz, Baja California Sur, México
2Department of Genomic and Environmental Toxicology Medicine. Toxicogenomic Laboratory. IIB-UNAM. Ciudad de México, México

Tóm tắt

Abstract

This study evaluated organismal toxicity, cytotoxicity, and genotoxicity and the filtration rate in response to different concentrations of subchronic lindane (gamma‐hexachlorocyclohexane [γ‐HCH]), exposure (12 d) in adult Pacific oysters Crassostrea gigas. Oysters were exposed in vivo in laboratory aquaria to 10 different concentrations (0.0–10.0 mg/L) of γ‐HCH. The median lethal concentration (LC50) after 12 d was calculated as 2.22 mg/L. Cytotoxic effects were observed in hemocytes, where the mean cell viability was significantly decreased at 1.0 mg/L of γ‐HCH after 12 d. Genotoxicity of γ‐HCH measured by single cell gel electrophoresis assay, in hemocytes was evident at 0.7 mg/L of γ‐HCH after 12 d. After 4 h of exposure to γ‐HCH, filtration rates were reduced compared with controls to 65.8 and 38.2% at concentrations of 0.3 and 0.7 mg/L, respectively, and after 11 d of exposure, filtration rates were reduced to 60.4 and 30.9% at concentrations of 0.1 mg/L and higher. These results show the subchronic effects of γ‐HCH at different concentrations and effect sensitivities are categorized as filtration rate < genotoxicity < cytotoxicity < mortality. The relevance of integral toxicity evaluation, considering different endpoints from molecular, cellular, and individual levels is discussed.

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Tài liệu tham khảo

10.1515/REVEH.2000.15.4.399

10.1016/j.envsci.2006.08.002

10.1080/09593332308618323

10.1007/s001280000013

International Agency for Research on Cancer, 1987, Overall evaluations of carcinogenicity, IARC monographs on the evaluation of carcinogenic risk of chemicals to humans, 20, 220

10.1007/s00244-004-0151-8

10.1016/j.marpolbul.2003.08.001

10.1016/S0300-483X(99)00226-7

10.1016/j.ecoenv.2003.07.014

10.1016/S0045-6535(99)00234-9

10.1016/j.chemosphere.2004.08.063

10.1007/s001289900776

10.1016/j.scitotenv.2006.04.031

10.1016/j.envpol.2004.05.035

10.1016/0742-8413(95)02062-4

10.1016/S0025-326X(98)00112-X

Rana K, 2001, Trends in global aquaculture production: 1984–1996. FAO Document Repository. United Nations Food and Agriculture Organization (FAO)

10.1111/j.1744-7348.1935.tb07166.x

10.1016/0014-4827(57)90013-7

10.3109/10520298709108028

10.1016/S0378-4347(98)00313-2

10.1385/MB:26:3:249

10.1080/00785326.1972.10430110

Zar JH, 1999, Biostatistical Analysis

10.1897/02-604

10.1002/etc.5620220226

10.1016/j.envpol.2003.09.027

10.1016/0742-8413(91)90153-K

10.1016/j.marenvres.2004.03.066

Das S, 2003, Oxygen uptake and filtration rate as animal health biomarker in Lamellidens marginalis (Lamarck), Indian J Exp Biol, 41, 1306

Udoidiong OM, 1991, Toxicity of cadmium, lead and lindane to Egeria radiata Lamarck (Lamellibranchia, Donacidae), Rev Hydrobiol Trop, 24, 111

10.1007/BF00011937

His E, 1993, Effects of twelve pesticides on larvae of oysters (Crassostrea gigas) and on two species of unicellular marine algae (Isochrysis galbana and Chaetoceros Calcitrans)

10.1006/eesa.1994.1034

10.1016/S0065-2881(08)60428-9

10.1007/s10565-006-0011-6

10.1016/j.marenvres.2003.11.004

10.3390/ijerph2004020090

10.1023/A:1016050911012

Rosa R, 1996, Cytotoxicity of hexachlorocyclohexane isomers and cyclodienes in primary cultures of cerebellar granule cells, J Pharmacol Exp Ther, 278, 163

10.1016/0003-9861(92)90453-4

10.1016/S0378-4274(99)00029-6

10.1016/S0025-326X(02)00254-0

10.1590/S1415-47572005000300023

10.1016/S1383-5718(00)00162-5

10.1016/j.aquatox.2005.08.001

10.1016/0278-6915(93)90077-C

10.1016/S1383-5742(03)00017-6

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Thông tin xuất bản

Environmental Toxicology and Chemistry

Tập 26 Số 10

2192-2197

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