Subcellular localization of five singular WSC domain‐containing proteins and their roles in Beauveria bassiana responses to stress cues and metal ions

Environmental Microbiology Reports - Tập 8 Số 2 - Trang 295-304 - 2016
Sen‐Miao Tong1, Ying Chen1, Jing Zhu1, Sheng‐Hua Ying1, Ming‐Guang Feng1
1Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China

Tóm tắt

SummarySome model fungi have three or four proteins with each vectoring a single cell Wall Stress‐responsive Component (WSC) domain at N‐terminus. In this study, five proteins, each vectoring only a single WSC domain in N‐terminal, central or even C‐terminal region, were found in Beauveria bassiana, a filamentous fungal entomopathogen, and named Wsc1A–1E due to the domain singularity. Four of them lack either transmembrane domain or C‐terminal conserved signature sequence (DXXD) compared with the homologues in the model fungi. Intriguingly, all the eGFP‐tagged fusion proteins of Wsc1A–1E were evidently localized to the cell wall and membrane of transgenic hyphae. Single deletions of the five wsc genes resulted in significant, but differential, increases in cellular sensitivity to cell wall perturbation, oxidation, high osmolarity, and four to six metal ions (Zn2+, Mg2+, Fe2+, K+, Ca2+ and Mn2+). Each deletion mutant also showed a delay of germination and a decrease of conidial UV‐B resistance, thermotolerance or both. However, none of the deletions affected substantially the fungal growth, conidiation and virulence. Our results indicate a significance of each WSC protein for the B. bassiana adaptation to diverse habitats of host insects.

Từ khóa


Tài liệu tham khảo

10.1089/omi.2010.0060

10.1371/journal.pone.0087948

10.1111/j.1365-2958.2011.07946.x

10.1038/nchembio.220

10.1016/j.jip.2003.12.003

10.1080/09583159409355309

10.1128/EC.05080-11

10.1093/emboj/16.16.4924

10.1002/yea.1155

10.1046/j.1365-2958.1999.01375.x

10.1038/nprot.2010.19

10.1371/journal.pone.0011104

10.1128/JB.181.11.3330-3340.1999

10.1111/j.1574-6976.2002.tb00613.x

10.1128/MMBR.69.2.262-291.2005

10.1534/genetics.111.128264

10.1007/BF03187215

10.1006/meth.2001.1262

10.1093/genetics/152.4.1487

10.1002/yea.320020304

10.1111/j.1574-6968.2006.00367.x

10.1371/journal.pone.0042374

10.1074/jbc.274.16.11296

10.1128/MCB.21.1.271-280.2001

10.1093/emboj/16.16.4924

10.1046/j.1365-2958.1999.01389.x

10.1128/MCB.19.6.3969

10.1016/j.fgb.2009.01.002

10.1016/S0065-2164(04)54001-7

10.1074/jbc.M604497200

10.1016/j.febslet.2007.08.027

10.1093/molbev/msr121

10.1073/pnas.94.25.13804

10.1007/s00438-010-0563-2

10.1038/srep00483

10.1371/journal.pone.0030298

10.1007/s00253-006-0447-x

Zu T., 2001, Mutations in WSC genes for putative stress receptors result in sensitivity to multiple stress conditions and impairment of Rlm1‐dependent gene expression in Saccharomyces cerevisiae, Mol Genet Genomics, 266, 142, 10.1007/s004380100537

Thông tin
Thông tin xuất bản

Environmental Microbiology Reports

Tập 8 Số 2

295-304

Thông tin tác giả