Study of transient luminescence of three kinds of Ru complexes bound to DNA
Tóm tắt
The transient luminescence of three kinds of ruthenium complexes [Ru(bpy)2(7-CH3-dppz)]2+, [Ru(bpy)2(7-F-dppz)]2+ and [Ru(phen)2(7-F-dppz)]2+ bound to calf thymus DNA (ctDNA) has been studied by using the time-resolved spectroscopy. The results show that the luminescence is due to the radiative decay from the charge-transfer states to the ground state. By the interaction with DNA, the radiativeless rate of the photoexcited Ru complex molecules decreases, which results in the increase of luminescence lifetime and efficiency. The structure of the Ru complex has an important impact on the interaction with DNA. The [Ru(bpy)2(7-CH3-dppz)]2+ shows the longest luminescence lifetime (about 382 ns), while the [Ru(bpy)2(7-F-dppz)]2+ shows the shortest lifetime (about 65 ns). The possible origin of the luminescence dynamics is discussed.
Tài liệu tham khảo
Chen X D, Jiang S H, Liu M H. DNA-based molecular device. Prog Chem (in Chinese), 2003, 15(4): 332–337
Ji L N, Zhang Q L, Liu J G. Development of the study on the structure, structural representation, interaction mechanism and biologic function of DNA in biomedicine. Sci China Ser B-Chem (in Chinese), 2001, 31(3): 193–204
Ji L N, Zhang Q L, Chao H. Function and application future of Polypyridyl complexes in macromolecule DNA. Chin Sci Bull (in Chinese), 2001, 46(6): 451–461
Friedman A E, Chambron J C, Sauvage J P, et al. Molecular “light switch” for DNA: Ru(bpy)2(dppz)2+. J Am Chem Soc, 1990, 112(12): 4960–4962
Hartshorn R M, Barton J K. Novel Dipyridophenazine complexes of Ruthenium (II): Exploring luminescent reporters of DNA. J Am Chem Soc, 1992, 114(15): 5919–5925
Liu X W, Li J, Deng H, et al. Experimental and DFT studies on the DNA-binding trend and spectral properties of complexes [Ru(bpy)2L]2+ (L = dmdpq, dpq, and dcdpq). Inorg Chim Acta, 2005, 358: 3311–3319
Han H Y, He Z K, Zeng Y E. Study on the chemiluminescent characteristics of mulitbipyridine ruthenium (II) complexe. Acta Chimica Sin (in Chinese), 2001, 59(9): 1513–1518
Deng H, Li H, Xu H, et al. Influence of ligand configuration and Hydrophobicity on DNA binding of polypyridyl Ruthenium (II) complexes. Acta Chimica Sin (in Chinese), 2002, 60(12): 2159–2166
Hiort C, Lincoln P, Nordén B. DNA binding of Δ-and Λ-[Ru(phen)2DPPZ]2+. J Am Chem Soc, 1993, 115(9): 3448–3454
Jenkins Y, Friedman A E, Turro N J, et al. Characterization of dipyridophenazine complexes of Ruthenium(II): the light switch effect as a function of nucleic acid sequence and conformation. Biochemistry, 1992, 31(44): 10809–10816
Ling L S, Yang X, He Z K, et al. Spectral studies on the interaction of DNA and Ru(bipy)2(dppz)2+. J Anal Sci (in Chinese), 2001, 17(1): 11–15
Westerlund F, Pierard F, Eng M P, et al. Enantioselective luminescence quenching of DNA light-switch [Ru(phen)2dppz]2+ by electron transfer to structural homologue [Ru(phendione)2dppz]2+. J Phys Chem B, 2005, 109(36): 17327–17332
Tysoe S A, Baker A D, Strekees T C. Spectroscopic investigation of differential binding modes of Δ-and Λ Ru(bpy)2 (dppz)2+ with calf thymus DNA. J Phys Chem, 1993, 97(8): 1707–1711
Schoonover J R, Bates W D, Meyer T J. Application of resonance Raman spectroscopy to electronic structure in metal complex excited states. Excited-state ordering and electron eelocalization in Dipyrido[3,2-∼:2′,3′-c]phenazine (dppz): Complexes of Re(I) and Ru(II). Inorg Chem, 1995, 34(26): 6421–6422
Turro C, Bossmann S H, Jenkins Y, et al. Proton transfer quenching of the MLCT excited state of Ru(phen)2dppz2+ in homogeneous solution and bound to DNA. J Am Chem Soc, 1995, 117(35): 9026–9032
Olson E J C, Hu D, Hörmann A, et al. First observation of the key intermediate in the “light-switch” mechanism of [Ru(phen)2dppz]2+. J Am Chem Soc, 1997, 119(47): 11458–11467