Study of IgM Aggregation in Serum of Patients with Macroglobulinemia

Clinical Chemistry and Laboratory Medicine - Tập 38 Số 8 - Trang 759-764 - 2000
Loretta Sharma1, Jon Baker2, Annabelle M. Brooks2, Ajit Kumar Sharma3
1Nursing Department, Great Lakes College, Midland, USA.
2Central Michigan Univ.#TAB#
3Chemistry & Biochemistry

Tóm tắt

AbstractThe effect of solvent conditions on the aggregation of IgM in serum specimens from patients with macroglobulinemia was studied by a turbidimetric procedure. Aggregation of IgM varied considerably among the samples and was affected by a number of experimental parameters. In general, IgM aggregation was more pronounced under acidic conditions and in solvents with low ionic strength. The presence of watermiscible organic solvents also promoted aggregation. Based on these studies, it was concluded that the major force involved in the formation of immunoglobulin aggregates in the serum of patients with macroglobulinemia was electrostatic, rather than hydrophobic, interactions. A number of additives known to prevent protein aggregation were evaluated for their effectiveness in inhibiting IgM aggregation. The only additives that were shown to inhibit or reduce IgM aggregation were charged molecules, such as arginine, sodium chloride, ethylenediamintetraacetic acid and quaternary ammonium β-cyclodextrin. Some of these charged additives were also effective in dissociating the IgM aggregates once they were formed, even in the presence of detergent.

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Thông tin xuất bản

Clinical Chemistry and Laboratory Medicine

Tập 38 Số 8

759-764

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