Studies on thiabendazole resistance of Penicillium expansum of pears: pathogenic fitness and genetic characterization

Plant Pathology - Tập 52 Số 3 - Trang 362-370 - 2003
Elena Baraldi1, Marta Mari1, Elena Chierici1, M. Pondrelli1, P. Bertolini1, G.C. Pratella1
1Dipartimento di Protezione e Valorizzazione Agroalimentare, CRIOF, Università di Bologna, Via Fanin 46, 40127 Bologna, Italy

Tóm tắt

Isolates of Penicillium expansum recovered from stored pears were scored for resistance to the fungicide thiabendazole (TBZ) and for pathogenic fitness. Out of 50 isolates, nine were sensitive (S) and 41 resistant (R or RR). Seven of these resistant isolates (RR) germinated with a higher percentage on TBZ‐amended medium than on unamended medium. Six S isolates and six RR isolates were chosen at random for further analysis. S and RR isolates had similar in vitro growth fitness, although RR isolates were characterized by higher infection severity on fruits. Laboratory‐induced resistant isolates were generated by UV‐irradiating S strains, and a similar correlation between the induced TBZ resistance and pathogenic fitness was observed. The β‐tubulin gene of RR and S isolates was amplified and sequenced; mutations correlating with TBZ resistance were identified at residues Phe 167 and Glu 198. Analogous mutations were detected in the laboratory‐induced resistant isolates.

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Tài liệu tham khảo

10.1016/S0007-1536(83)80017-5

Bolay A, 1974, The control of grey rot of grapes, Revue Suisse de Viticulture Arboriculture Horticulture, 6, 91

10.1021/ja01468a052

Bryk H, 1997, Appearance and stability of Pezicula alba Gunth. resistant to benzimidazole fungicides, Journal of Fruit and Ornamental Plant Research, 5, 77

Cargo CA, 1970, Thiabendazole and benomyl for the control of postharvest decay of apples, Hortscience, 5, 259, 10.21273/HORTSCI.5.4.259

10.1016/0048-3575(71)90209-4

10.1099/00221287-143-6-2003

10.1016/0048-3575(73)90030-8

De Brabander MJ, 1976, The effects of methyl (5‐(2‐thienylcarbonyl)‐1H‐benzimidazol‐2‐yl) carbamate, (R 17934; NSC 238159), a new synthetic antitumoral drug interfering with microtubules, on mammalian cells cultured in vitro, Cancer Research, 36, 905

Eaton LG, 1969, The veterinary uses of thiabendazole, Texas Reports on Biology and Medicine, 27, 693

10.1007/BF00351701

10.1099/00221287-67-3-371

10.1016/S0968-0004(98)01285-7

10.1002/cm.970170204

10.1002/cm.970220304

Koch AL, 1985, Biology of Industrial Organisms.

10.1094/Phyto-82-1348

Lee CU, 1985, Tolerance of apple blue mould (Penicillium expansum) to various fungicides, Korean Journal of Plant Pathology, 1, 128

10.1002/sim.4780100907

10.1016/0014-5793(96)00334-1

10.1128/MCB.6.7.2452

10.1091/mbc.11.5.1887

10.1094/PD-75-0712

10.1094/Phyto-85-103

10.1093/genetics/111.4.715

Warner G, 1993, Pear rot emerging as postharvest problem, Good-Fruit-Grower, 44, 25

WildBL 1983.Double resistance by citrus green mould Penicillium digitatumto the fungicide guazatine and benomyl.Annals of Applied Biology105 237–41.

Wilkinson L, 1990, systat. The System for Statistics

10.1094/Phyto-83-1478

10.2307/3761084

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Thông tin xuất bản

Plant Pathology

Tập 52 Số 3

362-370

Thông tin tác giả