Studies on the in vitro and in vivo antiurolithic activity of Holarrhena antidysenterica

Urological Research - Tập 40 - Trang 671-681 - 2012
Aslam Khan1,2, Saeed R. Khan3, Anwar H. Gilani2
1Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan
2Natural Product Research Division, Department of Biological and Biomedical Sciences, Aga Khan University Medical College, Karachi, Pakistan
3Centre for the Study of Lithiasis, Department of Pathology, College of Medicine, University of Florida, Gainesville, USA

Tóm tắt

Holarrhena antidysenterica has a traditional use in the treatment of urolithiasis, therefore, its crude extract has been investigated for possible antiurolithic effect. The crude aqueous-methanolic extract of Holarrhena antidysenterica (Ha.Cr) was studied using the in vitro and in vivo methods. In the in vitro experiments, Ha.Cr demonstrated a concentration-dependent (0.25–4 mg/ml) inhibitory effect on the slope of aggregation. It decreased the size of crystals and transformed the calcium oxalate monohydrate (COM) to calcium oxalate dehydrate (COD) crystals, in calcium oxalate metastable solutions. It also showed concentration-dependent antioxidant effect against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and lipid peroxidation induced in rat kidney tissue homogenate. Ha.Cr (0.3 mg/ml) reduced (p < 0.05) the cell toxicity and LDH release in renal epithelial cells (MDCK) exposed to oxalate (0.5 mM) and COM (66 μg/cm2) crystals. In male Wistar rats, receiving 0.75 % ethylene glycol (EG) for 21 days along with 1 % ammonium chloride (AC) in drinking water, Ha.Cr treatment (30–100 mg/kg) prevented the toxic changes caused by lithogenic agents; EG and AC, like loss of body weight, polyurea, oxaluria, raised serum urea and creatinine levels and crystal deposition in kidneys compared to their respective controls. These data indicate that Holarrhena antidysenterica possesses antiurolithic activity, possibly mediated through the inhibition of CaOx crystal aggregation, antioxidant and renal epithelial cell protective activities and may provide base for designing future studies to establish its efficacy and safety for clinical use.

Tài liệu tham khảo

Coe FL, Keck J, Norton ER (1977) The natural history of calcium urolithiasis. JAMA 238:1519–1523 Bashir S, Gilani AH, Siddiqui AA, Pervez S, Khan SR, Sarfaraz NJ, Shah AJ (2010) Berberis vulgaris root bark extract prevents hyperoxaluria induced urolithiasis in rats. Phytother Res 24:1250–1255 Hussain M, Rizvi SA, Askari H, Sultan G, Lal M, Ali B, Naqvi SA (2009) Management of stone disease: 17 years experience of a stone clinic in a developing country. J Pak Med Assoc 59:843–846 Srisubat A, Potisat S, Lojanapiwat B, Setthawong V, Laopaiboon M (2009) Extracorporeal shock wave lithotripsy (ESWL) versus percutaneous nephrolithotomy (PCNL) or retrograde intrarenal surgery (RIRS) for kidney stones. Cochrane Database Syst Rev CD007044 Qiang W, Ke Z (2004) Water for preventing urinary calculi. Cochrane Database Syst Rev CD004292 Coe FL, Evan AP, Worcester EM, Lingeman JE (2010) Three pathways for human kidney stone formation. Urol Res 38:147–160 Moe OW, Pearle MS, Sakhaee K (2011) Pharmacotherapy of urolithiasis: evidence from clinical trials. Kidney Int 79:385–392 Hess B (2003) Pathophysiology, diagnosis and conservative therapy in calcium kidney calculi. Ther Umsch 60:79–87 Mattle D, Hess B (2005) Preventive treatment of nephrolithiasis with alkali citrate—a critical review. Urol Res 33:73–79 Kmiecik J, Kucharska E, Sulowicz W, Ochmanski W (1997) Etiology and pathogenesis of urolithiasis. Przegl Lek 54:173–179 Khan A, Bashir S, Khan SR, Gilani AH (2011) Antiurolithic activity of Origanum vulgare is mediated through multiple pathways. BMC Complement Altern Med 11:96 Gilani AH, Rahman AU (2005) Trends in ethnopharmocology. J Ethnopharmacol 100:43–49 Butterweck V, Khan SR (2009) Herbal medicines in the management of urolithiasis: alternative or complementary? Planta Med 75:1095–1103 Usmanghani K, Saeed A, Alam MT (1997) Indusyunic medicine. University of Karachi Press, Karachi, pp 255–256 Duke JA, Bogenschutz-Godwin MJ, Du celliar J, Duke PK (2002) Handbook of medicinal herbs, 2nd edn. CRC Press, Boca Raton, p 219 Kapoor LD (1990) Hand book of ayurvedic medicinal plants. CRC Press, Boca Raton, pp 205–206 Duke JA (1992) Hand book of phytochemical constituents of GRAS herbs and other economic plants. CRC Press, Inc., Boca Raton, pp 292–293 Aqil F, Zahin M, Ahmad I (2008) Antimutagenic activity of methanolic extracts of four ayurvedic medicinal plants. Indian J Exp Biol 46:668–672 Aqil F, Ahmad I (2007) Antibacterial properties of traditionally used Indian medicinal plants. Methods Find Exp Clin Pharmacol 29:79–92 Atal CK, Sharma ML, Kaul A, Khajuria A (1986) Immunomodulating agents of plant origin. I: preliminary screening. J Ethnopharmacol 18:133–141 Gilani AH, Khan A, Khan AU, Bashir S, Rehman NU, Mandukhail SU (2010) Pharmacological basis for the medicinal use of Holarrhena antidysenterica in gut motility disorders. Pharm Biol 48:1240–1246 Khan A, Bashir S, Gilani AH (2012) An in vivo study on the diuretic activity of Holarrhena antidysenterica. Afr J Pharm Pharmacol 6:454–458 National Research Council (1996) Guide for the care and use of laboratory animals. National Academy Press, Washington, DC Williamson EM, Okpako DT, Evans FJ (1996) Selection, preparation, and pharmacological evaluation of plant material. Wiley, Chichester Bashir S, Gilani AH (2009) Antiurolithic effect of Bergenia ligulata rhizome: an explanation of the underlying mechanisms. J Ethnopharmacol 122:106–116 Ajith TA, Usha S, Nivitha V (2007) Ascorbic acid and [alpha]-tocopherol protect anticancer drug cisplatin induced nephrotoxicity in mice: a comparative study. Clin Chim Acta 375:82–86 Kang DG, Yun C, Lee HS (2003) Screening and comparison of antioxidant activity of solvent extracts of herbal medicines used in Korea. J Ethnopharmacol 87:231–236 Atmani F, Slimani Y, Mimouni M, Hacht B (2003) Prophylaxis of calcium oxalate stones by Herniaria hirsuta on experimentally induced nephrolithiasis in rats. BJU Int 92:137–140 Bashir S, Gilani AH (2011) Antiurolithic effect of berberine is mediated through multiple pathways. Eur J Pharmacol 651:168–175 Bashir S, Gilani AH (2011) Antiurolithic effect of berberine is mediated through multiple pathways. Eur J Pharmacol 651:168–175 Pizzolato P (1971) Mercurous nitrate as a histochemical reagent for calcium phosphate in bone and pathological calcification and for calcium oxalate. Histochem J 3:463–469 Vanachayangkul P, Chow N, Khan SR, Butterweck V (2010) Prevention of renal crystal deposition by an extract of Ammi visnaga L. and its constituents khellin and visnagin in hyperoxaluric rats. Urol Res 33:189–195 Daly JA, Ertingshausen G (1972) Direct method for determining inorganic phosphate in serum with the “CentrifiChem”. Clin Chem 18:263–265 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275 Hess B, Meinhardt U, Zipperle L, Giovanoli R, Jaeger P (1995) Simultaneous measurements of calcium oxalate crystal nucleation and aggregation: impact of various modifiers. Urol Res 23:231–238 Tiselius HG (2003) Epidemiology and medical management of stone disease. BJU Int 91:758–767 Guerra A, Meschi T, Allegri F, Prati B, Nouvenne A, Fiaccadori E, Borghi L (2006) Concentrated urine and diluted urine: the effects of citrate and magnesium on the crystallization of calcium oxalate induced in vitro by an oxalate load. Urol Res 34:359–364 Wesson JA, Ward MD (2006) Role of crystal surface adhesion in kidney stone disease. Curr Opin Nephrol Hypertens 15:386–393 Sheng X, Ward MD, Wesson JA (2005) Crystal surface adhesion explains the pathological activity of calcium oxalate hydrates in kidney stone formation. J Am Soc Nephrol 16:1904–1908 Wang AY (2009) Vascular and other tissue calcification in peritoneal dialysis patients. Perit Dial Int 29(Suppl 2):S9–S14 Khan SR (1997) Animal models of kidney stone formation: an analysis. World J Urol 15:236–243 Kato Y, Yamaguchi S, Yachiku S, Nakazono S, Hori J, Wada N, Hou K (2004) Changes in urinary parameters after oral administration of potassium-sodium citrate and magnesium oxide to prevent urolithiasis. Urology 63:7–11 (discussion 11–12) Gurocak S, Kupeli B (2006) Consumption of historical and current phytotherapeutic agents for urolithiasis: a critical review. J Urol 176:450–455 Atmani F, Khan SR (2000) Effects of an extract from Herniaria hirsuta on calcium oxalate crystallization in vitro. BJU Int 85:621–625 Aihara K, Byer KJ, Khan SR (2003) Calcium phosphate-induced renal epithelial injury and stone formation: involvement of reactive oxygen species. Kidney Int 64:1283–1291 Escobar C, Byer KJ, Khaskheli H, Khan SR (2008) Apatite induced renal epithelial injury: insight into the pathogenesis of kidney stones. J Urol 180:379–387 Byer K, Khan SR (2005) Citrate provides protection against oxalate and calcium oxalate crystal induced oxidative damage to renal epithelium. J Urol 173:640–646 Santhosh Kumar M, Selvam R (2003) Supplementation of vitamin E and selenium prevents hyperoxaluria in experimental urolithic rats. J Nutr Biochem 14:306–313 Thamilselvan S, Khan SR, Menon M (2003) Oxalate and calcium oxalate mediated free radical toxicity in renal epithelial cells: effect of antioxidants. Urol Res 31:3–9 Babich H (1982) Butylated hydroxytoluene (BHT): a review. Environ Res 29:1–29 Zahin M, Aqil F, Ahmad I (2009) The in vitro antioxidant activity and total phenolic content of four Indian medicinal plants International. J pharm pharm Sci 1:88–95 Park HK, Jeong BC, Sung MK, Park MY, Choi EY, Kim BS, Kim HH, Kim JI (2008) Reduction of oxidative stress in cultured renal tubular cells and preventive effects on renal stone formation by the bioflavonoid quercetin. J Urol 179:1620–1626 Muruganandan S, Srinivasan K, Gupta S, Gupta PK, Lal J (2005) Effect of mangiferin on hyperglycemia and atherogenicity in streptozotocin diabetic rats. J Ethnopharmacol 97:497–501 Michelacci YM, Boim MA, Bergamaschi CT, Rovigatti RM, Schor N (1992) Possible role for chondroitin sulfate in urolithiasis: in vivo studies in an experimental model. Clin Chim Acta 208:1–8 Fan J, Glass MA, Chandhoke PS (1999) Impact of ammonium chloride administration on a rat ethylene glycol urolithiasis model. Scanning Microsc 13:299–306 Tisselius HG (1996) Solution chemistry of supersaturation. In: Coe FL, Favus MJ, Pak CYC, Parks JH, Preminger GM (eds) Kidney stones: medical and surgical management. Lippincott-Raven, Philadelphia, pp 33–64 Soundararajan P, Mahesh R, Ramesh T, Begum VH (2006) Effect of Aerva lanata on calcium oxalate urolithiasis in rats. Indian J Exp Biol 44:981–986 Sangeeta D, Sidhu H, Thind SK, Nath R (1994) Effect of Tribulus terrestris on oxalate metabolism in rats. J Ethnopharmacol 44:61–66 Santhosh Kumar M, Selvam R (2003) Supplementation of vitamin E and selenium prevents hyperoxaluria in experimental urolithic rats. J Nutr Biochem 14:306–313 Wiessner JH, Hasegawa AT, Hung LY, Mandel GS, Mandel NS (2001) Mechanisms of calcium oxalate crystal attachment to injured renal collecting duct cells. Kidney Int 59:637–644 Patel MV, Patel KB, Gupta SN (2010) Effects of Ayurvedic treatment on forty-three patients of ulcerative colitis. Ayu 31:478–481 Pal A, Sharma PP, Mukherjee PK (2009) A Clinical Study of Kutaja (Holarrhena Antidysenterica Wall) on Shonitarsha. Hindu 5(33):33 Paranjpe P, Patki P, Joshi N (2000) Efficacy of an indigenous formulation in patients with bleeding piles: a preliminary clinical study. Fitoterapia 71:41–45