Studies on dynamic dissociation constant of 99Mo–insulin complex
Tóm tắt
In this article we studied the dynamic dissociation constant (k
d) of 99Mo complexed with insulin molecule at various pH. The k
d values were determined by dialysis technique against deionised water. The T
1/2 of the molybdenum–insulin complexes were found to be 6.41, 5.25 and 3.5 h at pH 5, 6 and 7 respectively. The half-lives indicate that insulin may act as good carrier of 99Mo to the intestine and may be useful in the field of nuclear medicine.
Tài liệu tham khảo
Panneerselvam SR, Govindasamy S (2004) Effect of sodium molybdate on the status of lipids, lipid peroxidation and antioxidant systems in alloxan-induced diabetic rats. Clin Chim Acta 345:93
Li J, Elberg G, Gefel D, Shechter Y (1995) Permolybdate and pertungstatepotent stimulators of insulin effects in rat adipocytes: mechanism of action. Biochemistry 34:6218
Goldwaser I, Gefel D, Gersonov E, Fridkin M, Shechter Y (2000) Insulin-like effects of vanadium: basic and clinical implications. J Inorg Biochem 80:21
Mulyani I, Levina A, Lay PA (2004) Biomimetic oxidation of chromium(III): does the antidiabetic activity of chromium(III) involve carcinogenic chromium(VI)? Angew Chem Int Ed 43:4504
Schroeder HA, Balassa JJ, Tipton IH (1970) Essential trace metals in man: molybdenum. J Chronic Dis 23:481
Lord SJ, Epstein NA, Paddock RL, Vogels CM, Hennigar TL, Zaworotko MJ, Taylor NJ, Driedzic WR, Broderick TL, Westcott SA (1999) Synthesis, characterization, and biological relevance of hydroxypyrone and hydroxipyridinone complex of molybdenum. Can J Chem 77:1249
Broderick TL, Bailey J, Gagnon KJ, Lord SJ, Vogels CM, Westcott SA (2006) Effect of a novel molybdenum ascorbate complex on ex vivo myocardial performance in chemical diabetes mellitus. Drugs R&D 7:119
ÖZcelikay AT, Becker DJ, Ongemba LN, Pottier AM, Henquin JC, Brichard SM (1996) Improvement of glucose and lipid metabolism in diabetic rats treated with molybdate. Am J Physiol 270:E344–E352
Nayak D, Lahiri S (2009) Immobilisation of no-carrier-added 93mMo on a biopolymer calcium alginate: a candidate radiopharmaceutical. J Radioanal Nucl Chem 281:181
Liu N, Yang Y, Zan L, Liao J, Jin J (2007) Astatine-211 labelling of insulin: synthesis and preliminary evaluation in vivo and in vitro. J Radioanal Nucl Chem 272:85
Moll UM, Thun Ch, Pfeiffer EF (1986) Colloidal gold-labelled insulin complex. Histochem Cell Biol 86:83
Orci L, Ravazzola M, Perrelet A (1984) (Pro)insulin associates with Golgi membranes of pancreatic B cells. Proc Natl Acad Sci USA 81:6743
Lahiri S, Roy K, Sen S (2008) Complexation study on no-carrier-added astatine with insulin: a candidate radiopharmaceutical. Appl Radiat Isot 66:1901
Roy K, Sen S, Lahiri S (2008) Studies on 198Au–insulin complex: a proposed radiopharmaceutical for targeted therapy. In: Collery Ph, Maymard I, Theophanides T, Khassanova L, Collery T (eds) Metal ions in biology and medicine, vol 10. John Libbey Eurotext, Paris
Lahiri S, Sarkar S (2008) Separation of no-carrier-added Tl and Pb radionuclides using poly(N-vinylpyrrolidone). J Radioanal Nucl Chem 277:513
Lahiri S, Sarkar S (2007) Studies on 66,67Ga and 199Tl-poly(N-vinylpyrolidone) complex. Appl Radiat Isot 65:309
Sarkar S, Nayak D, Lahiri S (2007) Studies on the interaction of poly(N-vinylpyrrolidone) with no-carrier-added Cu-61, Zn-62, Ga-66, Ge-69 and As-71 using tracer packet technique. Radiochim Acta 95:467
Miller JK, Moss BR, Bell MC, Sneed NN (1972) Comparison of 99Mo metabolism in young cattle and swine. J Anim Sci 34:846
Maiti M, Lahiri S (2010) Separation of 99Mo and 99mTc by liquid–liquid extraction using trioctylamine as extractant. J Radioanal Nucl Chem 283:661
Maiti M, Sen K, Sen S, Lahiri S (2011) Studies on stabilities of some human chorionic gonadotropin complexes with β-emitting radionuclides. Appl Radiat Isot 69:316
Helmerhorst E, Stokes GB (1987) Self-association of insulin. Its pH dependence and effect of plasma. Diabetes 36:261
Wierczinski B, Denkova AG, Peters JA, Wolterbeek HT (2006) Kinetic stability of metal complexes—determination of k a and k d using radiotracers. In: Lahiri S, Nayak D, Mukhopadhyay A (eds) Application of radiotracers in chemical, environmental and biological sciences. Saha Institute of Nuclear Physics, Kolkata
Lahiri S, Sarkar S (2007) Separation of iron and cobalt using 59Fe and 60Co by dialysis of polyvinylpyrrolidone-metal complexes: a greener approach. Appl Radiat Isot 65:387
Dressman JB, Berardi RR, Dermentzoglou LC, Russell TL, Schmaltz SP, Barnett JL, Jarvenpaa KM (1990) Upper gastrointestinal (GI) pH in young, healthy men and women. Pharm Res 7:756
http://www.medstardiabetes.org/documents/Insulin.pdf. Accessed 6 Dec 2011
Gimenez I, Garay R, Alda JO (1993) Molybdenum uptake through the anion exchanger in human erythrocytes. Eur J Physiol 424:245