Structure of a bacterial quorum-sensing transcription factor complexed with pheromone and DNA

Whitehead, N. A., Barnard, A. M. L., Slater, H., Simpson, N. J. L. & Salmond, G. P. C. Quorum-sensing in Gram-negative bacteria. FEMS Microbiol. Rev. 25, 365–404 (2001)

Fuqua, C., Parsek, M. R. & Greenberg, E. P. Regulation of gene expression by cell-to-cell communication: acyl-homoserine lactone quorum sensing. Annu. Rev. Genet. 35, 439–468 (2001)

Hanzelka, B. L. & Greenberg, E. P. Evidence that the N-terminal region of the Vibrio fischeri LuxR protein constitutes an autoinducer-binding domain. J. Bacteriol. 177, 815–817 (1995)

Choi, S. H. & Greenberg, E. P. Genetic evidence for multimerization of LuxR, the transcriptional regulator of Vibrio fischeri luminescence. Mol. Mar. Biol. Biotechnol. 1, 408–413 (1992)

Choi, S. H. & Greenberg, E. P. The C-terminal region of the Vibrio fischeri LuxR protein contains an inducer-independent lux gene-activating domain. Proc. Natl Acad. Sci. USA 88, 1115–1119 (1991)

Stevens, A. M., Dolan, K. M. & Greenberg, E. P. Synergistic binding of the Vibrio fischeri LuxR transcriptional activator domain and RNA polymerase to the lux promoter region. Proc. Natl Acad. Sci. USA 91, 12619–12623 (1994)

Fuqua, W. C. & Winans, S. C. A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumour metabolite. J. Bacteriol. 176, 2796–2806 (1994)

Egland, K. A. & Greenberg, E. P. Conversion of the Vibrio fischeri transcriptional activator, LuxR, to a repressor. J. Bacteriol. 182, 805–811 (2000)

Luo, Z. Q. & Farrand, S. K. Signal-dependent DNA binding and functional domains of the quorum-sensing activator TraR as identified by repressor activity. Proc. Natl Acad. Sci. USA 96, 9009–9014 (1999)

Chai, Y., Zhu, J. & Winans, S. C. A defective TraR-like protein of Agrobacterium tumefaciens forms heterodimers with TraR in vitro, thereby blocking TraR-mediated quorum sensing. Mol. Microbiol. 40, 414–421 (2001)

Zhu, J. & Winans, S. C. Autoinducer binding by the quorum-sensing regulator TraR increases affinity for target promoters in vitro and decreases traR turnover rates in whole cells. Proc. Natl Acad. Sci USA 96, 4832–4837 (1999)

Zhu, J. & Winans, S. C. The quorum-sensing regulator TraR of Agrobacterium tumefaciens requires autoinducer for protein folding, dimerization, and protease resistance. Proc. Natl Acad. Sci. USA 98, 1507–1512 (2001)

Qin, Y. et al. Quorum-sensing signal binding results in dimerization of TraR and its release from membranes into the cytoplasm. EMBO J. 19, 5212–5221 (2000)

Sandler, B. H., Nikonova, L., Leal, W. S. & Clardy, J. Sexual attraction in the silkworm moth: structure of the pheromone-binding-protein-bombykol complex. Chem. Biol. 7, 143–151 (2000)

Welch, M. et al. N-acyl homoserine lactone binding to the CarR receptor determines quorum-sensing specificity in Erwinia. EMBO J. 19, 631–641 (2000)

von Bodman, S. B., Majerczak, D. R. & Coplin, D. L. A negative regulator mediates quorum-sensing control of exopolysaccharide production in Pantoea stewartii subsp. Proc. Natl Acad. Sci. USA 95, 7687–7692 (1998)

Nelson, H. C. Structure and function of DNA-binding proteins. Curr. Opin. Genet. Dev. 5, 180–189 (1995)

Baikalov, I. et al. NarL dimerization? Suggestive evidence from a new crystal form. Biochemistry. 37, 3665–3676 (1998)

Luo, Z.-Q., Qin, Y. & Farrand, S. K. The antiactivator TraM interferes with the autoinducer-dependent binding of TraR to DNA by interacting with the C-terminal region of the quorum-sensing activator. J. Biol. Chem. 275, 7713–7722 (2000)

Egland, K. A. & Greenberg, E. P. Quorum sensing in Vibrio fischeri: analysis of the LuxR DNA binding region by alanine-scanning mutagenesis. J. Bacteriol. 183, 382–386 (2001)

Tempe, J., Petit, A., Holsters, M., Van Montagu, M. & Schell, J. Theromsensitive step associated with transfer of the Ti plasmid during conjugation: possible relation to transformation in crown gall. Proc. Natl Acad. Sci. USA 74, 2848–2849 (1977)

Joachimiak, A. & Sigler, P. B. Crystallization of protein-DNA complexes. Methods Enzymol. 208, 82–99 (1991)

Walsh, M. A., Dementieva, I., Evans, G., Sanishvili, R. & Joachimiak, A. Taking MAD to the extreme: ultrafast protein structure determination. Acta Crystallogr. D 55, 1168–1173 (1999)

Walsh, M. A., Evans, G., Sanishvili, R., Dementieva, I. & Joachimiak, A. MAD data collection—current trends. Acta Crystallogr. D 55, 1726–1732 (1999)

Otwinowski, Z. & Minor, W. Processing of x-ray diffraction data collected in oscillation mode. Methods Enzymol. 276, 307–326 (1997)

Brünger, A. et al. Crystallography & NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr. D 54, 905–921 (1998)

Perrakis, A., Morris, R. & Lamzin, V. S. Automated protein model building combined with iterative structure refinement. Nature Struct. Biol. 6, 458–463 (1999)

QUANTA, Molecular Simulations Inc, San Diego. (2000).