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Cấu trúc và phân tích chức năng dự đoán của cộng đồng vi sinh vật trong hai bộ lọc sinh học xử lý khí thải liên tục/không liên tục
Tóm tắt
Hai bộ lọc sinh học được vận hành ở chế độ liên tục (BTF1) và không liên tục (BTF2) với thời gian lưu giữ giường rỗng không đổi là 60 giây trong 60 ngày. Từ ngày thứ 60, chế độ vận hành của mỗi BTF đã được chuyển đổi trái ngược lại. Hiệu suất loại bỏ cao hơn cho năm chất ô nhiễm thơm đã được ghi nhận ở BTF1 (> 77.2%). Việc chuyển đổi chế độ vận hành không làm thay đổi hiệu suất loại bỏ của BTF1. So với đó, BTF2 không được thích ứng thành công trong chế độ vận hành không liên tục. Khi chế độ này được chuyển sang chế độ liên tục, hiệu suất loại bỏ của BTF2 đối với tất cả các chất ô nhiễm tăng lên đáng kể và cuối cùng vượt quá các giá trị quan sát được trong BTF1, với ngoại lệ duy nhất là p-xylene. Phân tích tọa độ nguyên tắc và phân tích sự tương đồng (ANOSIM) cho thấy cấu trúc của các cộng đồng vi sinh vật khác nhau rõ rệt giữa hai BTF (R = 1.000, p < 0.01) cũng như trước và sau khi chuyển đổi ở BTF2 (R = 0.996, p < 0.01). Mô hình rừng ngẫu nhiên cho thấy Mycobacterium, Burkholderia và Comamonas là ba chi vi khuẩn quan trọng nhất đóng góp vào sự khác biệt trong các cộng đồng vi sinh vật giữa hai BTF. Metagenomics được suy đoán qua PICUSt (khảo sát phát sinh của các cộng đồng bằng việc tái cấu trúc các trạng thái chưa quan sát) cho thấy BTF2 có tiềm năng phân hủy cao đối với các chất ô nhiễm thơm, mặc dù các gen liên quan đến việc hình thành biofilm ít hoạt động hơn ở BTF2 so với BTF1.
Từ khóa
#bộ lọc sinh học #vi sinh vật #hiệu suất loại bỏ #chất ô nhiễm thơm #phân tích cộng đồngTài liệu tham khảo
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