Structural, spectroscopic and cytotoxicity studies of TbF3@CeF3 and TbF3@CeF3@SiO2 nanocrystals

Springer Science and Business Media LLC - Tập 15 - Trang 1-15 - 2013
Tomasz Grzyb1, Marcin Runowski1, Krystyna Dąbrowska2, Michael Giersig1,3, Stefan Lis1
1Department of Rare Earths, Faculty of Chemistry, Adam Mickiewicz University, Poznan, Poland
2Bacteriophage Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland
3Institute of Experimental Physics, Freie Universität Berlin, Berlin, Germany

Tóm tắt

Terbium fluoride nanocrystals, covered by a shell, composed of cerium fluoride were synthesized by a co-precipitation method. Their complex structure was formed spontaneously during the synthesis. The surface of these core/shell nanocrystals was additionally modified by silica. The properties of TbF3@CeF3 and TbF3@CeF3@SiO2 nanocrystals, formed in this way, were investigated. Spectroscopic studies showed that the differences between these two groups of products resulted from the presence of the SiO2 shell. X-ray diffraction patterns confirmed the trigonal crystal structure of TbF3@CeF3 nanocrystals. High resolution transmission electron microscopy in connection with energy-dispersive X-ray spectroscopy showed a complex structure of the formed nanocrystals. Crystallized as small discs, ‘the products’, with an average diameter around 10 nm, showed an increase in the concentration of Tb3+ ions from surface to the core of nanocrystals. In addition to photo-physical analyses, cytotoxicity studies were performed on HSkMEC (Human Skin Microvascular Endothelial Cells) and B16F0 mouse melanoma cancer cells. The cytotoxicity of the nanomaterials was neutral for the investigated cells with no toxic or antiproliferative effect in the cell cultures, either for normal or for cancer cells. This fact makes the obtained nanocrystals good candidates for biological applications and further modifications of the SiO2 shell. .

Tài liệu tham khảo

Abel K, Boyer JC, Van Veggel FCJM (2009) Hard proof of the NaYF4/NaGdF4 nanocrystal core/shell structure. J Am Chem Soc 131:14644–14655. doi:10.1021/ja906971y Bao H, Yang J, Huang Y, Xu ZP, Hao N, Wu Z, Lu GQM, Zhao D (2011) Synthesis of well-dispersed layered double hydroxide core@ordered mesoporous silica shell nanostructure (LDH@mSiO2) and its application in drug delivery. Nanoscale 3:4069–4073. doi:10.1039/c1nr10718f Bogdan N, Vetrone F, Ozin Ga, Capobianco Ja (2011) Synthesis of ligand-free colloidally stable water dispersible brightly luminescent lanthanide-doped upconverting nanoparticles. Nano Lett 11:835–840. doi:10.1021/nl1041929 Boulon G (2012) Fifty years of advances in solid-state laser materials. Opt Mater 34:499–512. doi:10.1016/j.optmat.2011.04.018 Carnall WT, Goodman GL, Rajnak K, Rana RS (1989) A systematic analysis of the spectra of the lanthanides doped into single crystal LaF3. J Chem Phys 90:3443–3457. doi:10.1063/1.455853 Chai RT, Lian HZ, Li CX, Cheng ZY, Hou ZY, Huang SS, Lin J (2009) In situ preparation and luminescent properties of CeF3 and CeF3:Tb3+ nanoparticles and transparent CeF3:Tb3+/PMMA Nanocomposites in the visible spectral range. J Phys Chem C 113:8070–8076. doi:10.1021/Jp900269b Cheetham AK, Fender BEF, Fuess H, Wright AF (1976) A powder neutron diffraction study of lanthanum and cerium trifluorides. Acta Crystallogr B 32:94–97. doi:10.1107/S0567740876002380 Chen DQ, Yu YL, Huang P, Lin H, Shan ZF, Wang YS (2010a) Color-tunable luminescence of Eu3+ in LaF3 embedded nanocomposite for light emitting diode. Acta Mater 58:3035–3041. doi:10.1016/j.actamat.2010.01.035 Chen SY, Lu YH, Huang TW, Yan DC, Dong C-L (2010b) Oxygen vacancy dependent magnetism of CeO2 nanoparticles prepared by thermal decomposition method. J Phys Chem C 114:19576–19581. doi:10.1021/jp1045172 Crola Da Silva C, Lamerant-Fayel N, Paprocka M, Mitterrand M, Gosset D, Dus D, Kieda C (2009) Selective human endothelial cell activation by chemokines as a guide to cell homing. Immunology 126:394–404. doi:10.1111/j.1365-2567.2008.02906.x Cubeddu R, Comelli D, D’Andrea C, Taroni P, Valentini G (2002) Time-resolved fluorescence imaging in biology and medicine. J Phys D Appl Phys 35:61–76. doi:10.1088/0022-3727/35/9/201 Di W, Li J, Shirahata N, Sakka Y, Willinger MG, Pinna N (2011a) Photoluminescence, cytotoxicity and in vitro imaging of hexagonal terbium phosphate nanoparticles doped with europium. Nanoscale 3:1263–1269. doi:10.1039/c0nr00673d Di W, Ren X, Zhao H, Shirahata N, Sakka Y, Qin W (2011b) Single-phased luminescent mesoporous nanoparticles for simultaneous cell imaging and anticancer drug delivery. Biomaterials 32:7226–7233. doi:10.1016/j.biomaterials.2011.06.019 Diamente PR, Burke RD, Van Veggel FC (2006) Bioconjugation of Ln3+-doped LaF3 nanoparticles to avidin. Langmuir 22:1782–1788. doi:10.1021/la052589r Evanics F, Diamente PR, Van Veggel FCJM, Stanisz GJ, Prosser RS (2006) Water-soluble GdF3 and GdF3/LaF3 nanoparticles physical characterization and NMR relaxation properties. Chem Mater 18:2499–2505. doi:10.1021/cm052299w Fu YX, Sun YH (2009) Comparative study of synthesis and characterization of monodispersed SiO2@Y2O3:Eu3+ and SiO2 @ Y2O3:Eu3+@SiO2 core–shell structure phosphor particles. J Alloys Compd 471:190–196. doi:10.1016/j.jallcom.2008.03.055 Ghosh P, De la Rosa E, Oliva J, Solis D, Kar A, Patra A, Rosa ED, Haldar KK (2008) Enhancement of upconversion Emission of LaPO4:Er@Yb Core–Shell nanoparticles/nanorods. J Phys Chem C 112:9650–9658. doi:10.1021/jp801978b Grzyb T, Lis S (2011) Structural and spectroscopic properties of LaOF:Eu3+ nanocrystals prepared by the sol–gel Pechini method. Inorg Chem 50:8112–8120. doi:10.1021/ic2005453 Grzyb T, Runowski M, Szczeszak A, Lis S (2012a) Influence of matrix on the luminescent and structural properties of glycerine-capped, Tb3+-doped fluoride nanocrystals. J Phys Chem C 116:17188–17196. doi:10.1021/jp3010579 Grzyb T, Szczeszak A, Rozowska J, Legendziewicz J, Lis S (2012b) Tunable luminescence of Sr2CeO4:M2+ (M=Ca, Mg, Ba, Zn) and Sr2CeO4:Ln3+ (Ln=Eu, Dy, Tm) nanophosphors. J Phys Chem C 116:3219–3226. doi:10.1021/jp208015z Grzyb T, Gruszeczka A, Wiglusz RJ, Śniadecki Z, Idzikowski B, Lis S (2012c) Multifunctionality of GdPO4:Yb3+, Tb3+ nanocrystals–luminescence and magnetic behaviour. J Mater Chem 22:22989–22997. doi:10.1039/c2jm34863b Grzyb T, Runowski M, Szczeszak A, Lis S (2013) Structural, morphological and spectroscopic properties of Eu3+-doped rare earth fluorides synthesized by hydrothermal method. J Solid State Chem 200:76–83. doi:10.1016/j.jssc.2013.01.012 Guo H (2006) Photoluminescent properties of CeF3:Tb3+ nanodiskettes prepared by hydrothermal microemulsion. Appl Phys B 84:365–369. doi:10.1007/s00340-006-2326-7 Guo C, Ding X, Seo HJ, Ren Z, Bai J (2011) Luminescent properties of UV excitable blue emitting phosphors MSr4(BO3)3:Ce3+ (M=Li and Na). J Alloys Compd 509:4871–4874. doi:10.1016/j.jallcom.2011.01.194 Henglein A, Giersig M (1999) Formation of colloidal silver nanoparticles: capping action of citrate. J Phys Chem B 103:9533–9539. doi:10.1021/jp9925334 Hu D, Chen M, Gao Y, Li F, Wu L (2011) A facile method to synthesize superparamagnetic and up-conversion luminescent NaYF4:Yb, Er/Tm@SiO2@Fe3O4 nanocomposite particles and their bioapplication. J Mater Chem 21:11276–11282. doi:10.1039/c1jm11172h Janowski M, Bulte JWM, Walczak P (2012) Personalized nanomedicine advancements for stem cell tracking. Adv Drug Deliv Rev 64:1488–1507. doi:10.1016/j.addr.2012.07.008 Ji JY, Shih PH, Yang CC, Chan TS, Ma YR, Wu SY (2010) Spontaneous self-organization of Cu2O/CuO core–shell nanowires from copper nanoparticles. Nanotechnology 21:45603. doi:10.1088/0957-4484/21/4/045603 Johnson NJJ, Oakden W, Stanisz GJ, Prosser RS, Van Veggel FCJM (2011) Size-tunable, ultrasmall NaGdF4 nanoparticles: insights into their T1 MRI contrast enhancement. Chem Mater 23:3714–3722. doi:10.1021/cm201297x Kang X, Cheng Z, Li C, Yang D, Shang M, Ma P, Li G, Liu N, Lin J (2011) Core–shell structured up-conversion luminescent and mesoporous NaYF4:Yb3+/Er3+@nSiO2@mSiO2 nanospheres as carriers for drug delivery. J Phys Chem C 115:15801–15811. doi:10.1021/jp203039t Karbowiak M, Mech A, Bednarkiewicz A, Strek W, Kepinski L (2005) Comparison of different NaGdF4: Eu3+ synthesis routes and their influence on its structural and luminescent properties. J Phys Chem Solids 66:1008–1019. doi:10.1016/j.jpcs.2005.01.002 Kunath W, Zemlin F, Weiss K (1987) Refinement procedures for high-resolution electron microscopy. Optik 76:122–131 Lin W, Huang YW, Zhou XD, Ma Y (2006) In vitro toxicity of silica nanoparticles in human lung cancer cells. Toxicol Appl Pharmacol 217:252–259. doi:10.1016/j.taap.2006.10.004 Park MVDZ, Neigh AM, Vermeulen JP, De la Fonteyne LJJ, Verharen HW, Briedé JJ, Van Loveren H, De Jong WH (2011) The effect of particle size on the cytotoxicity, inflammation, developmental toxicity and genotoxicity of silver nanoparticles. Biomaterials 32:9810–9817. doi:10.1016/j.biomaterials.2011.08.085 Park Y, Kim HM, Kim JH, Moon KC, Yoo B, Lee KT, Lee N, Choi Y, Park W, Ling D, Na K, Moon WK, Choi SH, Park HS, Yoon S-Y, Suh YD, Lee SH, Hyeon T (2012) Theranostic probe based on lanthanide-doped nanoparticles for simultaneous in vivo dual-modal imaging and photodynamic therapy. Adv Mater 24:5755–5761. doi:10.1002/adma.201202433 Philipse AP, Van Bruggen MPB, Pathmamanoharan C (1994) Magnetic silica dispersions: preparation and stability of surface-modified silica particles with a magnetic core. Langmuir 10:92–99. doi:10.1021/la00013a014 Qiu S (2000) Synthesis of CeF3 nanoparticles from water-in-oil microemulsions. Powder Technol 113:9–13. doi:10.1016/S0032-5910(99)00249-1 Qiu H, Chen G, Sun L, Hao S, Han G, Yang C (2011) Ethylenediaminetetraacetic acid (EDTA)-controlled synthesis of multicolor lanthanide doped BaYF5 upconversion nanocrystals. J Mater Chem 21:17202–17208. doi:10.1039/c1jm12950c Ren W, Tian G, Zhou L, Yin W, Yan L, Jin S, Zu Y, Li S, Gu Z, Zhao Y (2012) Lanthanide ion-doped GdPO4 nanorods with dual-modal bio-optical and magnetic resonance imaging properties. Nanoscale 4:3754–3760. doi:10.1039/c2nr30683b Ricci PC, Salis M, Corpino R, Carbonaro CM, Fortin E, Anedda A (2010) A kinetics model for Tb3+ recombinations in low doped Tb:Lu1.8Y0.2SiO5 crystals. J Appl Phys 108:43512–43517. doi:10.1063/1.3467788 Runowski M, Grzyb T, Lis S (2012) Magnetic and luminescent hybrid nanomaterial based on Fe3O4 nanocrystals and GdPO4:Eu3+ nanoneedles. J Nanopart Res 14:1185–1188. doi:10.1007/s11051-012-1188-7 Selvan ST, Tan TTY, Yi DK, Jana NR (2009) Functional and multifunctional nanoparticles for bioimaging and biosensing. Langmuir 26:11631–11641. doi:10.1021/la903512m Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR (1990) New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst 82:1107–1112. doi:10.1093/jnci/82.13.1107 Tang Z, Kotov N, Giersig M (2002) Spontaneous organization of single CdTe nanoparticles into luminescent nanowires. Science 297:237–240. doi:10.1126/science.1072086 Bedekar V, Dutta DPD, Mohapatra M, Godbole SV, Ghildiyal R, Tyagi AK, SV (2009) Rare-earth doped gadolinia based phosphors for potential multicolor and white light emitting deep UV LEDs. Nanotechnology 20: 125707-125717. doi:10.1088/0957-4484/20/12/125707 Väisänen V, Härmä H, Lilja H, Bjartell A (2000) Time-resolved fluorescence imaging for quantitative histochemistry using lanthanide chelates in nanoparticles and conjugated to monoclonal antibodies. Luminescence 15:389–397. doi:10.1002/1522-7243(200011/12)15:6<389:AID-BIO626>3.0.CO;2-7 Wang F, Zhang Y, Fan X, Wang M (2006a) Facile synthesis of water-soluble LaF3:Ln3 + nanocrystals. J Mater Chem 16:1031–1034. doi:10.1039/b518262j Wang ZL, Quan ZW, Jia PY, Lin CK, Luo Y, Chen Y, Fang J, Zhou W, O’Connor CJ, Lin J (2006b) Facile synthesis and photoluminescent properties of redispersible CeF3, CeF3: Tb3+, and CeF3:Tb3+/LaF3 (core/shell) nanoparticles. Chem Mater 18:2030–2037. doi:10.1021/Cm052360x Wang Y, Qin W, Zhang J, Cao C, Lü S, Ren X (2009) Photoluminescence of colloidal YVO4:Eu/SiO2 core/shell nanocrystals. Opti Commun 282:1148–1153. doi:10.1016/j.optcom.2008.12.007 Wang F, Han Y, Lim CS, Lu Y, Wang J, Xu J, Chen H, Zhang C, Hong M, Liu X (2010) Simultaneous phase and size control of upconversion nanocrystals through lanthanide doping. Nature 463:1061–1065. doi:10.1038/nature08777 Wang G, Peng Q, Li Y (2011a) Lanthanide-doped nanocrystals: synthesis, optical-magnetic properties, and applications. Acc Chem Res 44:322–332. doi:10.1021/ar100129p Wang M, Abbineni G, Clevenger A, Mao C, Xu S (2011b) Upconversion nanoparticles: synthesis, surface modification and biological applications. Nanomedicine 7:710–729. doi:10.1016/j.nano.2011.02.013 Wang Q, Bao Y, Zhang X, Coxon PR, Jayasooriya U, Chao Y (2012) Uptake and toxicity studies of poly-acrylic acid functionalized silicon nanoparticles in cultured mammalian cells. Adv Healthcare Mater 1:189–198. doi:10.1002/adhm.201100010 Wiglusz R, Grzyb T, Lis S, Strek W (2009) Preparation and spectroscopy characterization of Eu: MgAl2O4 nanopowder prepared by modified Pechini method. J Nanosci Nanotechnol 9:5803–5810. doi:10.1166/jnn.2009.1259 Yan ZG, Yan C (2008) Controlled synthesis of rare earth nanostructures. J Mater Chem 18:5046–5059. doi:10.1039/b810586c Yang X, Liu J, He H, Zhou L, Gong C, Wang X, Yang L, Yuan J, Huang H, He L, Zhang B, Zhuang Z (2010) SiO2 nanoparticles induce cytotoxicity and protein expression alteration in HaCaT cells. Part Fibre Toxicol 7:1–12. doi:10.1186/1743-8977-7-1 Yang D, Kang X, Shang M, Li G, Peng C, Li C, Lin J (2011) Size and shape controllable synthesis and luminescent properties of BaGdF5:Ce3+/Ln3+ (Ln = Sm, Dy, Eu, Tb) nano/submicrocrystals by a facile hydrothermal process. Nanoscale 3:2589–2595. doi:10.1039/c1nr10203f Zhang YW, Sun X, Si R, You LP, Yan C-H (2005) Single-crystalline and monodisperse LaF3 triangular nanoplates from a single-source precursor. J Am Chem Soc 127:3260–3271. doi:10.1021/ja042801y Zhu L, Meng J, Cao XQ (2008) Synthesis and photoluminescent properties of silica-coated LaCeF3:Tb nanocrystals. J Nanopart Res 10:383–386. doi:10.1007/s11051-007-9262-2