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Mô hình cấu trúc và sự điều chỉnh môi trường của arginine decarboxylase trong Synechocystis sp. PCC 6803
Tóm tắt
Arginine decarboxylase (ADC) là enzyme đầu tiên trong con đường thay thế để tổng hợp putrescine trong con đường sinh tổng hợp polyamine ở vi khuẩn và thực vật. Trong nghiên cứu này, chúng tôi đã tập trung vào tác động của các loại stress ngắn hạn khác nhau đối với lượng bản sao và hoạt tính cụ thể của ADC Synechocystis sp. PCC 6803. Kết quả của chúng tôi cho thấy sự tích lũy bản sao trong trạng thái ổn định và hoạt động enzyme không liên quan đến nhau theo cách đơn giản, vì chỉ có quá trình quang heterotrophy và sự kết hợp giữa stress muối và stress ánh sáng mạnh tác động đến cả hai tham số một cách tương tự. Sự thay đổi trong sự tích lũy mRNA ADC trong trạng thái ổn định dưới các điều kiện stress ngắn hạn khác mà chúng tôi đã nghiên cứu chỉ có tác động nhỏ đến hoạt tính enzyme, cho thấy sự điều chỉnh sau phiên mã. Dựa trên mô hình cấu trúc, ADCs của Synechocystis có một miền dư thuyết, có thể tham gia vào việc điều chỉnh sau phiên mã hoạt động của ADC trong Synechocystis. Ngoài ra, hai liên kết disulfide đối xứng giữa các tiểu đơn vị dường như ổn định cấu trúc dimer của ADCs. Có hai gen mã hóa cho ADC và agmatinase, một enzyme khác trong con đường polyamine, trong bộ gen của Synechocystis, trong khi các gen mã hóa cho ornithine decarboxylase và một số enzyme khác trong con đường polyamine chưa được xác định thông qua các tìm kiếm tính đồng nhất.
Từ khóa
#argine decarboxylase #Synechocystis #enzyme #stress ngắn hạn #điều chỉnh sau phiên mã #mô hình cấu trúcTài liệu tham khảo
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