Structural Studies on the Coat Protein of Alfalfa‐Mosaic Virus

FEBS Journal - Tập 28 Số 1 - Trang 20-29 - 1972
Barend Kraal1, J.Martien de Graaf1, Ton A. Bakker1, Gerard M. A. VAN BEYNUM1, Menno Goedhart1, Leendert Bosch1
1Biochemisch Laboratorium, Rijksuniversiteit Wassenaarseweg 64, Leiden, The Netherlands

Tóm tắt

The investigation described in this paper deals with various aspects of the primary structure of the coat protein of the plant virus alfalfa‐mosaic virus (AMV 425). The N‐terminus of this protein has been identified as N‐acetylserine, the C‐terminal structure is ‐Arg‐His · COOH. Cyanogen bromide cleavage of the reduced and carboxymethylated protein yields four major peptide fragments which is in accordance with the presence of three methionine residues in the intact protein chain. These fragments have been separated and characterized by amino acid analysis, electrophoretic behaviour on polyacrylamide gel, end‐group determination and molecular weight. Together they account for all the amino acid residues in the entire protein chain. The alignment of the peptide fragments along the chain is presented. The data lend strong support to the molecular size of this protein reported previously from this laboratory.

Từ khóa


Tài liệu tham khảo

Vloten‐Doting L., 1968, Virology, 34, 728, 10.1016/0042-6822(68)90093-7

Moed J. R., 1968, Virology, 36, 459, 10.1016/0042-6822(68)90171-2

10.1016/S0022-2836(67)80123-2

10.1016/0042-6822(71)90007-9

10.1016/0042-6822(69)90224-4

10.1016/0042-6822(71)90192-9

Hagedorn D. J., 1963, Phytopathology, 53, 188

10.1021/bi00779a015

Black L. W., 1969, J. Biol. Chem., 244, 1976, 10.1016/S0021-9258(18)94355-3

10.1016/0005-2795(68)90116-5

10.1016/0003-9861(69)90069-1

10.1016/S0076-6879(67)11037-9

10.1016/0003-2697(71)90436-2

10.1016/0006-291X(71)90762-5

10.1016/0003-2697(69)90123-7

10.1016/0006-291X(69)90263-0

Power T. F., 1970, Anal. Biochem., 36, 537, 10.1016/0003-2697(70)90392-1

10.1016/0006-3002(58)90445-1

10.1016/0006-291X(69)90953-X

10.1016/0304-4165(65)90147-9

10.1016/0003-2697(69)90301-7

10.1111/j.1432-1033.1969.tb19632.x

10.1016/S0021-9673(00)99170-2

Jentsch J., 1968, Z. Naturforsch., 23, 1613, 10.1515/znb-1968-1215

10.1042/bj0680475

VanRavenswaay Claasen J. C.(1967)Ph.D. Thesis University of Leiden.

10.1042/bj0980567

Kraal B.(1972)Ph.D. Thesis Universits of Leiden.

Thông tin
Thông tin xuất bản

FEBS Journal

Tập 28 Số 1

20-29

Thông tin tác giả