Streptomyces vilmorinianum sp. nov. Isolated from Soil of Zhengzhou
Tóm tắt
Streptomyces vilmorinianum sp. nov. (CGMCC.23383), is aerobic, gram-positive-staining bacterium isolated from soil of Zhengzhou, Henan Province in China. Granaticin was revealed among the secondary metabolites of S. vilmorinianum YP1 by high performance liquid chromatography (HPLC) and ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS-MS). Strain YP1 grew in a mineral medium with lactose and peptone. Whole-cell hydrolysates of strain YP1 contained LL-diaminopimelic acid, glucose and ribose. The major isoprenoid quinones were MK-9 (H8) and MK-9 (H6). The characteristic polar lipids were phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylinositol mannoside (PIM), and an unidentified lipid. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YP1 was closely related to Streptomyces lateritius LMG 19372T (NBCR 12788T) and Streptomyces zaomyceticus NBRC 13348T. The DNA–DNA hybridization (dDDH) values of strain YP1 and the closely related type strains were 28.5 and 33.9%, respectively. The average nucleotide identity (ANI) values were 83.95 and 87.56%, respectively. Based on the polyphasic study of this bacterium, we proposed a novel species of the genus Streptomyces, Streptomyces vilmorinianum with the strain YP1.
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