Stimulation Effects of Glutamic and 5-Aminolevulinic Acids On Photosynthetic Pigments, Physio-biochemical Constituents, Antioxidant Activity, and Yield of Peanut

I. M. El-Metwally1, Mervat Sh. Sadak1, Hani Saber Saudy2
1Botany Department, Agricultural and Biological Research Institute, National Research Centre, El-Behos St. Dokki, P.O. Box 12622-Dokki, Cairo, Egypt
2Agronomy Department, Faculty of Agriculture, Ain Shams University, P.O. Box 68-Hadayek Shoubra, 11241, Cairo, Egypt

Tóm tắt

AbstractSoil not only represents the main supporter for root growth, but also is the supplier of water and nutrients. However, several soils, i.e. sandy soils, do not adequately fulfill the plant growth requirements of the environmental resources. Therefore, it is necessary to compensate, even partially, the lack of these required resources for better plant growth and development. Amino acids could introduce a substantial solution in this respect. Therefore, two field experiments under field conditions were carried out to investigate the effect of glutamic (GLA) and 5‑aminolevulinic (ALA) acids on photosynthesis pigments, oxidative defense indicators as well as yield and seed quality of peanut. Three concentrations of glutamic acid (10, 20 and 40 mg L−1, denoted GLA10, GLA20, and GLA40, respectively) and three concentrations of 5‑aminolevulinic acid, (10, 20 and 40 mg L−1, abbreviated to ALA10, ALA20, and ALA40, respectively), in addition to a check treatment (tap water) were applied. Treatments were arranged in a randomized complete block design with three replicates. Findings exhibited potentiality of GLA20 treatment for recording the highest values of chlorophyll a, chlorophyll b, chlorophyll a/b, carotenoids and total pigments compared to the other treatments. The increases in indole acetic acid, phenolics and free amino acids were 68.1, 58.9 and 19.6% as well as 64.6, 51.2 and 17.7%, due to application of GLA20 and ALA20, respectively. Substantial improvements in pod yield ha−1, oil %, flavonoids and antioxidant activity were obtained with GLA20 or ALA20. In conclusion, since glutamic or 5‑aminolevulinic acids at concentration of 20 mg L−1 showed promotive effect on physiological and biochemical status of peanut, such amino acids should be adopted as a promising practice in peanut cultivations.

Từ khóa


Tài liệu tham khảo

Abd–Elrahman ShH, Saudy HS, Abd El–Fattah DA, Hashem FA (2022) Effect of irrigation water and organic fertilizer on reducing nitrate accumulation and boosting lettuce productivity. J Soil Sci Plant Nutr. https://doi.org/10.1007/s42729-022-00799-8

Alfosea-Simón M, Zavala-Gonzalez EA, Camara-Zapata JM, Martínez-Nicolás JJ, Simón I, Simón-Grao S, García-Sánchez F (2020) Effect of foliar application of amino acids on the salinity tolerance of tomato plants cultivated under hydroponic system. Sci Hortic 272:109509. https://doi.org/10.1016/j.scienta.2020.109509

Ali B, Gill RA, Yang S, Gill MB, Farooq MA, Liu D, Daud MK, Ali S, Zhou W (2015) Regulation of cadmium-induced proteomic and metabolic changes by 5‑aminolevulinic acid in leaves of Brassica napus L. PLoS ONE 10:e123328. https://doi.org/10.1371/journal.pone.0123328

Amin AA, Gharib FAE, El-Awadia M, Rashad ESM (2011) Physiological response of onion plants to foliar application of putrescine and glutamine. Sci Hortic 129:353–360. https://doi.org/10.1016/j.scienta.2011.03.052

An YY, Cheng DX, Rao ZX, Sun YP, Tang Q, Wang LJ (2019) 5‑Aminolevulinic acid (ALA) promotes primary root elongation through modulation of auxin transport in Arabidopsis. Acta Physiol Plant 41:1–11. https://doi.org/10.1007/s11738-019-2878-x

An YY, Qi L, Wang LJ (2016) ALA pretreatment improves waterlogging tolerance of fig plants. PLoS ONE 11:e147202. https://doi.org/10.1371/journal.pone.0147202

AOAC (2012) Association of official agriculture chemists. Official methods of analysis, 19th edn. W. Hormitz, Washington

Bakry AB, Sadak MSh, Abd El-Monem AA (2020) Physiological aspects of tyrosine and salicylic acid on morphological, yield and biochemical constituents of peanut plants. Pak J Biol Sci 23:375–384. https://doi.org/10.3923/pjbs.2020.375.384

Beale SI (1990) Biosynthesis of the tetrapyrrole pigment precursor, δ‑Aminolevulinicacid, from glutamate. Plant Physiol 93:1273–1279. https://doi.org/10.1104/pp.93.4.1273

Bulgari R, Franzoni G, Ferrante A (2019) Biostimulants application in horticultural crops under abiotic stress conditions. Agron 9:306. https://doi.org/10.3390/agronomy9060306

Calvo P, Nelson L, Kloepper JW (2014) Agricultural uses of plant biostimulants. Plant Soil 383:3–41. https://doi.org/10.1007/s11104-014-2131-8

Cao YP, Gao ZK, Li JT, Xu GH, Wang M (2010) Effects of extraneous glutamic acid on nitrate contents and quality of Chinese chive. Acta Hortic 856:91–98. https://doi.org/10.17660/ActaHortic.2010.856.11

Casella G (2008) Statistical Design, 1st edn. Springer, Gainesville

Colla G, Rouphael Y (2015) Biostimulants in horticulture. Sci Hortic 196:1–2. https://doi.org/10.1016/j.scienta.2015.10.044

Czarnecki O, Grimm B (2012) Post-translational control of tetrapyrrole biosynthesis in plants, algae, and cyanobacteria. J Exp Bot 63:675–1687. https://doi.org/10.1093/jxb/err437

Danil AD, George CM (1972) Peach seed dormancy in relation to endogenous inhibitors and applied growth substances. J Am Soc Hortic Sci 17:621–624

El-Bially MA, Saudy HS, El-Metwally IM, Shahin MG (2018) Efficacy of ascorbic acid as a cofactor for alleviating water deficit impacts and enhancing sunflower yield and irrigation water-use efficiency. Agric Water Manag 208:132–139. https://doi.org/10.1016/j.agwat.2018.06.016

El-Bially MA, Saudy HS, El-Metwally IM, Shahin MG (2022a) Sunflower response to application of L‑ascorbate under thermal stress associated with different sowing dates. Gesunde Pflanz 74:87–96. https://doi.org/10.1007/s10343-021-00590-2

El-Bially MA, Saudy HS, Hashem FA, El-Gabry YA, Shahin MG (2022b) Salicylic acid as a tolerance inducer of drought stress on sunflower grown in sandy soil. Gesunde Pflanz. https://doi.org/10.1007/s10343-022-00635-0

El-Metwally IM, Saudy HS (2021) Interactional impacts of drought and weed stresses on nutritional status of seeds and water use efficiency of peanut plants grown in arid conditions. Gesunde Pflanz 73:407–416. https://doi.org/10.1007/s10343-021-00557-3

El-Metwally IM, Saudy HS, Abdelhamid MT (2021) Efficacy of benzyladenine for compensating the reduction in soybean productivity under low water supply. Ital J Agrometeorol 2:81–90. https://doi.org/10.36253/ijam-872

El-Metwally IM, Geries L, Saudy HS (2022) Interactive effect of soil mulching and irrigation regime on yield, irrigation water use efficiency and weeds of trickle–irrigated onion. Archiv Agron Soil Sci. https://doi.org/10.1080/03650340.2020.1869723

Fabbrin EGS, Mógor ÁF, Margoti G, Fowler JG, Bettoni MM (2013) Purple chicory ‘palla rossa’ seedlings growth according to the foliar application of L‑glutamic acid. Sci Agrar 14:91–94

Fahimi F, Souri MK, Yaghobi F (2016) Growth and development of greenhouse cucumber under foliar application of biomin and humifolin fertilizers in comparison to their soil application and NPK. J Sci Technol Greenh Cult 7:143–152. https://doi.org/10.18869/acadpub.ejgcst.7.1.143

Farid M, Farid S, Zubair M, Ghani MA, Rizwan M, Ishaq HK, Alkahtani S, Abdel-Daim MM, Ali S (2020) Glutamic acid-assisted phytomanagement of chromium contaminated soil by sunflower (Helianthus annuus L.): Morphophysiological and biochemical alterations. Front Plant Sci 11:1297. https://doi.org/10.3389/fpls.2020.01297

Fu J, Sun Y, Chu X, Xu Y, Hu T (2014) Exogenous 5‑aminolevulenic acid promotes seed germination in Elymus nutans against oxidative damage induced by cold stress. PLoS ONE 9:e107152. https://doi.org/10.1371/journal.pone.0107152

Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930. https://doi.org/10.1016/j.plaphy.2010.08.016

Gururani MA, Upadhyaya CP, Strasser RJ, Woong YJ, Park SW (2012) Physiological and biochemical responses of transgenic potato plants with altered expression of PSII manganese stabilizing protein. Plant Physiol Biochem 58:182–194. https://doi.org/10.1016/j.plaphy.2012.07.003

Gyamfi MA, Yonamine M, Aniya Y (1999) Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguine on experimentally-induced liver injuries. Gen Pharmacol 32:661–667. https://doi.org/10.1016/s0306-3623(98)00238-9

Hossain MA, Hoque MA, Burritt DJ, Fujita M (2014) Proline protects plants against abiotic oxidative stress: biochemical and molecular mechanisms. In: Ahmad P (ed) Oxidative damage to plants: antioxidant networks and signaling. Academic Press, New York, Boston, London, Oxford, pp 477–522

Jackson ML (1973) Soil chemical analysis. Prentice Hall, New Delhi

Khan AS, Ahmad B, Jaskani MJ, Ahmad R, Malik AU (2012) Foliar application of mixture of amino acids and seaweed (Ascophylum nodosum) extract improve growth and physicochemical properties of grapes. Int J Agric Biol 14:383–388

Krishna G, Singh BK, Kim EK, Morya VK, Ramteke PW (2015) Progress in genetic engineering of peanut (Arachis hypogaea L.): a review. Plant Biotechnol J 13:147–162. https://doi.org/10.1111/pbi.12339

Lafarga T (2021) Production and consumption of oils and oilseeds. In: Lafarga T, Bobo G, Aguilo-Aguayo I (eds) Oil and oilseed processing: opportunities and challenges. John Wiley & Sons Inc., Hoboken, pp 1–21 https://doi.org/10.1002/9781119575313.ch1

Larsen P, Harbo A, Klungsöyr S, Aashein T (1962) On the biosynthesis of some indole compounds in Acetobacter Xylinum. Physiol Plant 15:552–565. https://doi.org/10.1111/j.1399-3054.1962.tb08058.x

Lee HJ, Kim JS, Lee SG, Kim SK, Mun B, Choi CS (2017) Glutamic acid foliar application enhances antioxidant enzyme activities in kimchi cabbages treated with low air temperature. Hortic Sci Technol 35:700–706. https://doi.org/10.12972/kjhst.20170074

Levene H (1960) Robust tests of equality of variances. In: Olkin I, Ghurye SG, Hoeffding W, Madow WG, Mann HB (eds) Contributions to probability and statistics, essays in honor of harold hotelling. Stanford University Press, Stanford, pp 278–292

Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. Curr Protoc Food Anal Chem 1:F4.3.1–F4.3.8. https://doi.org/10.1002/0471142913.faf0403s01

Liu D, Pei ZF, Naeem MS, Ming DF, Liu HB, Khan F, Zhou WJ (2011) 5‑aminolevulinic acid activates antioxidative defense system and seedling growth in Brassica napus L. under water-deficit stress. J Agron Crop Sci 197:284–295. https://doi.org/10.1111/j.1439-037X.2011.00465.x

Lv DG, Yu C, Yang L, Qin SJ, Ma HY, Du GD, Liu GC, Khanizadeh S (2009) Effects of foliar-applied L‑glutamic acid on the diurnal variations of leaf gas exchange and chlorophyll fluorescence parameters in hawthorn (Crataegus pinnatifida Bge.). Eur J Hortic Sci 74:204–209

Ma Q, Cao X, Xie Y, Xiao H, Tan X, Wu L (2017) Effects of glucose on the uptake and metabolism of glycine in pakchoi (Brassica chinensis L.) exposed to various nitrogen sources. BMC Plant Biol 17:58. https://doi.org/10.1186/s12870-017-1006-6

Mahalingam R, Fedoroff N (2003) Stress response, cell death and signalling: the many faces of reactive oxygen species. Physiol Plant 119:56–68. https://doi.org/10.1034/j.1399-3054.2003.00156.x

Makhlouf BSI, Khalil SRA, Saudy HS (2022) Efficacy of humic acids and chitosan for enhancing yield and sugar quality of sugar beet under moderate and severe drought. J Soil Sci Plant Nutr. https://doi.org/10.1007/s42729-022-00762-7

Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9:490–498. https://doi.org/10.1016/j.tplants.2004.08.009

Mohammadipour N, Souri MK (2019a) Effects of different levels of glycine in the nutrient solution on the growth, nutrient composition and antioxidant activity of coriander (Coriandrum sativum L.). Acta Agrobot 72:1759. https://doi.org/10.5586/aa.1759

Mohammadipour N, Souri MK (2019b) Beneficial effects of glycine on growth and leaf nutrient concentrations of coriander (Coriandrum sativum) plants. J Plant Nutr 42:1637–1644. https://doi.org/10.1080/01904167.2019.1628985

Mubarak M, Salem EMM, Kenawey MKM, Saudy HS (2021) Changes in calcareous soil activity, nutrient availability, and corn productivity due to the integrated effect of straw mulch and irrigation regimes. J Soil Sci Plant Nutr 21:2020–2031. https://doi.org/10.1007/s42729-021-00498-w

Naeem MS, Warusawitharana H, Liu HB, Liu D, Ahmad R, Waraich EA, Xu L, Zhou WJ (2012) 5‑aminolevulinic acid alleviates the salinity-induced changes in Brassica napus as revealed by the ultrastructural study of chloroplast. Plant Physiol Bioch 57:84–92. https://doi.org/10.1016/j.plaphy.2012.05.018

Noroozlo YA, Souri MK, Delshad M (2019) Stimulation effects of foliar applied glycine and glutamine amino acids on lettuce growth. Open Agric 4:164–172

Nunkaew T, Kantachote D, Kanzaki H, Nitoda T, Ritchie RJ (2014) Effects of 5‑aminolevulinic acid (ALA)—containing supernatants from selected Rhodopseudomonas palustris strains on rice growth under NaCl stress, with mediating effects on chlorophyll, photosynthetic electron transport and antioxidative enzymes. Electron J Biotechnol 17:19–26. https://doi.org/10.1016/j.ejbt.2013.12.004

Okumoto S, Funck D, Trovato M, Forlani G (2016) Amino acids of the glutamate family: Functions beyond primary metabolism. Front Plant Sci 7:318. https://doi.org/10.3389/fpls.2016.00318

Ordoñez AAL, Gomez JD, Vattuone MA, Lsla MI (2006) Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food Chem 97:452–458. https://doi.org/10.1016/j.foodchem.2005.05.024

Pastori GM, Kiddle G, Antoniw J, Bernard S, Veljovic-Jovanovic S, Verrier PJ, Noctor G, Foyer CH (2003) Leaf vitamin C contents modulate plant defense transcripts and regulate genes that control development through hormone signaling. Plant Cell 15:939–951. https://doi.org/10.1105/tpc.010538

Rai VK (2002) Role of amino acids in plant responese to stresses. Biol plant 45:481–487. https://doi.org/10.1023/A:1022308229759

Röder C, Mógor ÁF, Szilagyi-Zecchin VJ, Gemin LG, Mógor G (2018) Potato yield and metabolic changes by use of biofertilizer containing L‑glutamic acid. Comun Sci 9:211–218. https://doi.org/10.14295/cs.v9i2.2564

Sadak MS, Ramadan AA (2021) Impact of melatonin and tryptophan on water stress tolerance in white lupine (Lupinus termis L.). Physiol Mol Biol Plants 27:469–481. https://doi.org/10.1007/s12298-021-00958-8

Sadak MS, Abdoelhamid MT, Schmidhalter U (2015) Effect of foliar application of amino acids on plant yield and some physiological parameters in bean plants irrigated with sea water. Acta Biol Colomb 20:141–152. https://doi.org/10.15446/abc.v20n1.42865

Salem EMM, Kenawey MKM, Saudy HS, Mubarak M (2021) Soil mulching and deficit irrigation effect on sustainability of nutrients availability and uptake, and productivity of maize grown in calcareous soils. Commun Soil Sci Plant Anal 52:1745–1761. https://doi.org/10.1080/00103624.2021.1892733

Salem EMM, Kenawey MKM, Saudy HS, Mubarak M (2022) Influence of silicon forms on nutrient accumulation and grain yield of wheat under water deficit conditions. Gesunde Pflanzen. https://doi.org/10.1007/s10343-022-00629-y

Saudy HS, El-Metwally IM (2019) Nutrient utilization indices of NPK and drought management in groundnut under sandy soil conditions. Comm Soil Sci Plant Anal 50:1821–1828. https://doi.org/10.1080/00103624.2019.1635147

Saudy HS, El-Bially MA, El-Metwally IM, Shahin MG (2021) Physio-biochemical and agronomic response of ascorbic acid-treated sunflower (Helianthus annuus) grown at different sowing dates and under various irrigation regimes. Gesunde Pflanz 73:169–179. https://doi.org/10.1007/s10343-020-00535-1

Saudy HS, El-Metwally IM, Abd El-Samad GA (2020a) Physio-biochemical and nutrient constituents of peanut plants under bentazone herbicide for broad-leaved weed control and water regimes in dry land areas. J Arid Land 12:630–639. https://doi.org/10.1007/s40333-020-0020-y

Saudy HS, Hamed MF, Abd El-Momen WR, Hussein H (2020b) Nitrogen use rationalization and boosting wheat productivity by applying packages of humic, amino acids and microorganisms. Commun Soil Sci Plant Anal 51:1036–1047. https://doi.org/10.1080/00103624.2020.1744631

Scholz FW, Stephens MA (1987) K‑Sample Anderson-Darling tests. J Am Stat Assoc 82:918–924. https://doi.org/10.1080/01621459.1987.10478517

Senge M, Ryan A, Letchford K, Macgowan S, Mielke T (2014) Chlorophylls, symmetry, chirality, and photosynthesis. Symmetry 6:781–843. https://doi.org/10.3390/sym6030781

Shahid M, Shamshad S, Rafiq M, Khalid S, Bibi I, Niazi NK, Dumat C, Rashid MI (2017) Chromium speciation, bioavailability, uptake, toxicity and detoxification in soil-plant system: a review. Chemosphere 178:513–533. https://doi.org/10.1016/j.chemosphere.2017.03.074

Shams M, Yildirim E, Ekinci M, Turan M, Dursun A, Parlakova F, Kul R (2016) Exogenously applied glycine betaine regulates some chemical characteristics and antioxidative defence systems in lettuce under salt stress. Hortic Environ Biotechnol 57:225–231. https://doi.org/10.1007/s13580-016-0021-0

Sharma SS, Dietz KJ (2006) The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress. J Exp Bot 57:711–726. https://doi.org/10.1093/jxb/erj073

Sharma P, Jha AB, Dubey RS, Pessarakli M (2012) Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J Bot. https://doi.org/10.1155/2012/217037

Souri MK (2016) Aminochelate fertilizers: the new approach to the old problem; a review. Open Agric 1:118–123. https://doi.org/10.1515/opag-2016-0016

Souri MK, Hatamian M (2019) Aminochelates in plant nutrition: a review. J Plant Nutr 42:67–78. https://doi.org/10.1080/01904167.2018.1549671

Souri MK, Yaghoubi F, Moghadamyar M (2017) Growth and quality of cucumber, tomato, and green bean plants under foliar and soil applications of an aminochelate fertilizer. Hortic Environ Biotechnol 58:530–536. https://doi.org/10.1007/s13580-017-0349-0

Svennerstam H, Ganeteg U, Bellini C, Näsholm T (2008) Root uptake of cationic amino acids by Arabidopsis depends on functional expression of amino acid permease 5. New Phytol 180:620–630. https://doi.org/10.1111/j.1469-8137.2008.02589.x

Teixeira WF, Soares LH, Fagan EB, da Costa MS, Reichardt K, Dourado-Neto D (2020) Amino acids as stress reducers in soybean plant growth under different water-deficit conditions. J Plant Growth Regul 39:905–919. https://doi.org/10.1007/s00344-019-10032-z

Vartainan N, Hervochon P, Marcotte L, Larher F (1992) Proline accumulation during drought rhizogenesis in Brassica napus var. oleifera. J Plant Physiol 140:623–628. https://doi.org/10.1016/S0176-1617(11)80799-6

Venkatesh J, Park SW (2014) Role of L‑ascorbate in alleviating abiotic stresses in crop plants. Bot Stud 55:38. https://doi.org/10.1186/1999-3110-55-38

Wang J, Zhang J, Li J, Dawuda MM, Ali B, Wu Y, Yu J, Tang Z, Lyu J, Xiao X, Hu L, Xie J (2021) Exogenous application of 5‑aminolevulinic acid promotes coloration and improves the quality of tomato fruit by regulating carotenoid metabolism. Front Plant Sci 12:683868. https://doi.org/10.3389/fpls.2021.683868

Wang LJ, Jiang WB, Huang BJ (2004) Promotion of 5‑aminolevulinic acid on photosynthesis of melon (Cucumis melo) seedlings under low light and chilling stress conditions. Physiol Plant 121:258–264. https://doi.org/10.1111/j.0031-9317.2004.00319.x

Wang YX, Wei SM, Wang JN, Su XY, Suo B, Qin FJ, Zhao HJ (2018) Exogenous application of 5‑ aminolevulinic acid on wheat seedlings under drought stress enhances the transcription of psbA and psbD genes and improves photosynthesis. Braz J Bot 41:275–285. https://doi.org/10.1007/s40415-018-0455-y

Wu Y, Liao WB, Dawuda MM, Hu LL, Yu JH (2019) 5‑Aminolevulinic acid (ALA) biosynthetic and metabolic pathways and its role in higher plants: a review. Plant Growth Regul 88:327. https://doi.org/10.1007/s10725-018-0463-8

Xu L, Zhang W, Ali B, Islam F, Zhu J, Zhou W (2015) Synergism of herbicide toxicity by 5‑aminolevulinic acid is related to physiological and ultrastructural disorders in crickweed (Malachium aquaticum L.). Pest Biochem Physiol 125:53–61. https://doi.org/10.1016/j.pestbp.2015.06.002

Yemm EW, Cocking EC (1955) The determination of amino acids with ninhydrin. Analyst 80:209–214. https://doi.org/10.1039/AN9558000209

Youssef T, Awad MA (2008) Mechanisms of enhancing photosynthetic gas exchange in date palm seedlings (Phoenix dactylifera L.) under salinity stress by a 5-aminolevulinic acid-based fertilizer. J Plant Growth Regul 27:1–9. https://doi.org/10.1007/s00344-007-9025-4

Zhang J, Li DM, Gao Y, Yu B, Xia CX, Bai JG (2012) Pretreatment with 5‑aminolevulinic acid mitigates heat stress of cucumber leaves. Biol Plant 56:780–784. https://doi.org/10.1007/s10535-012-0136-9

Zulfiqar F, Akram NA, Ashraf M (2020) Osmoprotection in plants under abiotic stresses: new insights into a classical phenomenon. Planta 251:3. https://doi.org/10.1007/s00425-019-03293-1