Stepwise Gradient Elution in RP HPTLC with a New Horizontal Developing Chamber

A general equation for determination of relative position, Rpg, of solutes chromatographed under conditions of stepwise gradient elution with one void volume of mobile phase in reverse-phase thinlayer chromatography has been applied to separation of ten component dye mixture. A satisfactory agreement between calculated (by computer program) and experimental values of Rpg has been demonstrated. A device with the new horizontal developing chamber has been applied to the investigations. This device showed practical advantages in comparison to other chambers especially in respect of its application to stepwise gradient elution.