Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics

Molecular & Cellular Proteomics - Tập 1 Số 5 - Trang 376-386 - 2002
Shao‐En Ong1, Blagoy Blagoev2, Irina Kratchmarova2, Dan Bach Kristensen3, Hanno Steen2, Akhilesh Pandey2, Matthias Mann2
1Protein Interaction Laboratory, University of Southern Denmark, Odense, Denmark
2Protein Interaction Laboratory, University of Southern Denmark, Odense M, DK-5230, Denmark
3MDS-Proteomics A/S, Staermosegaardsvej 6, Odense M, DK-5230, Denmark

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Tài liệu tham khảo

O’Farrell, 1975, High resolution two-dimensional electrophoresis of proteins, J. Biol. Chem., 250, 4007, 10.1016/S0021-9258(19)41496-8

Klose, 1995, Two-dimensional electrophoresis of proteins: an updated protocol and implications for a functional analysis of the genome, Electrophoresis., 16, 1034, 10.1002/elps.11501601175

Gorg, 2000, The current state of two-dimensional electrophoresis with immobilized pH gradients, Electrophoresis., 21, 1037, 10.1002/(SICI)1522-2683(20000401)21:6<1037::AID-ELPS1037>3.0.CO;2-V

Herbert, 2001, What place for polyacrylamide in proteomics?, Trends Biotechnol., 19, 3, 10.1016/S0167-7799(01)00002-6

Patton, 2002, Rainbow’s end: the quest for multiplexed fluorescence quantitative analysis in proteomics, Curr. Opin. Chem. Biol., 6, 63, 10.1016/S1367-5931(01)00284-8

Zhou, 2002, 2D differential in-gel electrophoresis for the identification of esophageal scans cell cancer-specific protein markers, Mol. Cell. Proteomics., 1, 117, 10.1074/mcp.M100015-MCP200

Browne, 1981, Kinetic equivalence of stable-isotope-labeled and unlabeled phenytoin, Clin. Pharmacol. Ther., 29, 511, 10.1038/clpt.1981.71

Shevchenko, 1997, Rapid “de novo” peptide sequencing by a combination of nanoelectrospray, isotopic labeling and a quadrupole/time-of-flight mass spectrometer, Rapid Commun. Mass Spectrom., 11, 1015, 10.1002/(SICI)1097-0231(19970615)11:9<1015::AID-RCM958>3.0.CO;2-H

Schnolzer, 1996, Protease-catalyzed incorporation of 18O into peptide fragments and its application for protein sequencing by electrospray and matrix-assisted laser desorption/ionization mass spectrometry, Electrophoresis., 17, 945, 10.1002/elps.1150170517

Uttenweiler-Joseph, 2001, Automated de novo sequencing of proteins using the differential scanning technique, Proteomics., 1, 668, 10.1002/1615-9861(200104)1:5<668::AID-PROT668>3.0.CO;2-S

Mirgorodskaya, 2000, Quantitation of peptides and proteins by matrix-assisted laser desorption/ionization mass spectrometry using (18)O-labeled internal standards, Rapid Commun. Mass Spectrom., 14, 1226, 10.1002/1097-0231(20000730)14:14<1226::AID-RCM14>3.0.CO;2-V

Yao, 2001, Proteolytic 18O labeling for comparative proteomics: model studies with two serotypes of adenovirus, Anal. Chem., 73, 2836, 10.1021/ac001404c

Larsen, 2001, Characterization of differently processed forms of enolase 2 from Saccharomyces cerevisiae by two-dimensional gel electrophoresis and mass spectrometry, Electrophoresis., 22, 566, 10.1002/1522-2683(200102)22:3<566::AID-ELPS566>3.0.CO;2-T

Stewart, 2001, 18O labeling: a tool for proteomics, Rapid Commun. Mass Spectrom., 15, 2456, 10.1002/rcm.525

Lahm, 2000, Mass spectrometry: a tool for the identification of proteins separated by gels, Electrophoresis., 21, 2105, 10.1002/1522-2683(20000601)21:11<2105::AID-ELPS2105>3.0.CO;2-M

Oda, 1999, Accurate quantitation of protein expression and site-specific phosphorylation, Proc. Natl. Acad. Sci. U. S. A., 96, 6591, 10.1073/pnas.96.12.6591

Veenstra, 2000, Proteome analysis using selective incorporation of isotopically labeled amino acids, J. Am. Soc. Mass Spectrom., 11, 78, 10.1016/S1044-0305(99)00120-8

Gygi, 1999, Quantitative analysis of complex protein mixtures using isotope-coded affinity tags, Nat. Biotechnol., 17, 994, 10.1038/13690

Han, 2001, Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry, Nat. Biotechnol., 19, 946, 10.1038/nbt1001-946

Zhou, 2002, Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry, Nat. Biotechnol., 20, 512, 10.1038/nbt0502-512

Munchbach, 2000, Quantitation and facilitated de novo sequencing of proteins by isotopic N-terminal labeling of peptides with a fragmentation-directing moiety, Anal. Chem., 72, 4047, 10.1021/ac000265w

Cagney, 2002, De novo peptide sequencing and quantitative profiling of complex protein mixtures using mass-coded abundance tagging, Nat. Biotechnol., 20, 163, 10.1038/nbt0202-163

Goodlett, 2001, Differential stable isotope labeling of peptides for quantitation and de novo sequence derivation, Rapid Commun. Mass Spectrom., 15, 1214, 10.1002/rcm.362

Regnier, 2002, Comparative proteomics based on stable isotope labeling and affinity selection, J. Mass Spectrom., 37, 133, 10.1002/jms.290

Oda, 2001, Enrichment analysis of phosphorylated proteins as a tool for probing the phosphoproteome, Nat. Biotechnol., 19, 379, 10.1038/86783

Zhou, 2001, A systematic approach to the analysis of protein phosphorylation, Nat. Biotechnol., 19, 375, 10.1038/86777

Goshe, 2001, Phosphoprotein isotope-coded affinity tag approach for isolating and quantitating phosphopeptides in proteome-wide analyses, Anal. Chem., 73, 2578, 10.1021/ac010081x

Shevchenko, 1996, Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels, Anal. Chem., 68, 850, 10.1021/ac950914h

Pandey, 2000, Use of mass spectrometry to study signaling pathways, Sci. STKE., 2000, PL1, 10.1126/stke.2000.37.pl1

Wilm, 1996, Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry, Nature., 379, 466, 10.1038/379466a0

Gobom, 1999, Sample purification and preparation technique based on nano-scale reversed-phase columns for the sensitive analysis of complex peptide mixtures by matrix-assisted laser desorption/ionization mass spectrometry, J. Mass Spectrom., 34, 105, 10.1002/(SICI)1096-9888(199902)34:2<105::AID-JMS768>3.0.CO;2-4

Mann, 1994, Error-tolerant identification of peptides in sequence databases by peptide sequence tags, Anal. Chem., 66, 4390, 10.1021/ac00096a002

Galbiati, 1999, J. Biol. Chem., 274, 30315, 10.1074/jbc.274.42.30315

Odelberg, 2000, Dedifferentiation of mammalian myotubes induced by msx1, Cell., 103, 1099, 10.1016/S0092-8674(00)00212-9

Jehn, 2002, c-Cbl binding and ubiquitin-dependent lysosomal degradation of membrane-associated Notch1, J. Biol. Chem., 277, 8033, 10.1074/jbc.M108552200

Dominov, 1998, Bcl-2 expression identifies an early stage of myogenesis and promotes clonal expansion of muscle cells, J. Cell Biol., 142, 537, 10.1083/jcb.142.2.537

Melo, 1996, Extracellular matrix is required for skeletal muscle differentiation but not myogenin expression, J. Cell. Biochem., 62, 227, 10.1002/(SICI)1097-4644(199608)62:2<227::AID-JCB11>3.0.CO;2-I

Kawachi, 2001, Dendritic cells presenting pyruvate kinase M1/M2 isozyme peptide can induce experimental allergic myositis in BALB/c mice, J. Neuroimmunol., 117, 108, 10.1016/S0165-5728(01)00327-7

Serini, 1998, The fibronectin domain ED-A is crucial for myofibroblastic phenotype induction by transforming growth factor-β1, J. Cell Biol., 142, 873, 10.1083/jcb.142.3.873