Xác định độ ổn định của dẫn xuất cyclized 2,4-dinitrophenyl hydrazone của glucose

Journal of Analytical Science and Technology - Tập 8 - Trang 1-8 - 2017
Muhammed Alzweiri1,2, Sara Al-Marabeh1,3, Sanaa K. Bardaweel1, Rawhi Alfar4, Yusuf M. Al-Hiari1
1Department of Pharmaceutical sciences, Faculty of Pharmacy, The University of Jordan, Amman, Jordan
2Faculty of Pharmacy, Al-Zaytoonah Private University of Jordan, Amman, Jordan
3Pharmaceutical Sciences Department, Faculty of Pharmacy, Zarqa University, Zarqa, Jordan
4Department of Physics, School of Science, Al-Balqa Applied University, Salt, Jordan

Tóm tắt

Chiến thuật được thiết lập tốt nhất để phân tích monosaccharide, chẳng hạn như glucose, dựa trên các quy trình chuyển hóa, sử dụng các tác nhân như 2,4-dinitrophenylhydrazine (DNPH). Thông thường, sự không ổn định của sản phẩm imine được hình thành làm suy giảm khả năng phát hiện lượng đường trong mẫu nhỏ; gây khó khăn cực kỳ cho phân tích quang phổ của monosaccharide. Trong nghiên cứu này, chúng tôi đề xuất một quy trình chuyển hóa đã được điều chỉnh, dựa trên sự hình thành dẫn xuất DNPH-glucose ổn định, nhằm hỗ trợ trong việc phân tích quang phổ của glucose. Quy trình chuyển hóa đã được tùy chỉnh để thực hiện bước xử lý sản phẩm trong điều kiện axit. Môi trường giàu proton dẫn đến việc giảm trực tiếp base Schiff với sự sắp xếp lại nội phân tử đồng thời của sản phẩm để tạo ra dẫn xuất DNPH-glucose cyclized ổn định. Cấu trúc đồng nhất của hợp chất được đề cập đã được xác minh bằng 1NMR, 13C-NMR, HMBC và tinh thể tia X. Dẫn xuất cho thấy độ ổn định mở rộng trong các mẫu huyết tương bị nhiễm, điều này cho thấy tiềm năng áp dụng quy trình được mô tả để phân tích và phát hiện glucose trong các mẫu sinh học.

Từ khóa

#glucose #2 #4-dinitrophenylhydrazine #dẫn xuất cyclized #phân tích quang phổ #độ ổn định

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Journal of Analytical Science and Technology

Tập 8

1-8

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