Spontaneous Recurrent Seizures in Rats: Amino Acid and Monoamine Determination in the Hippocampus

Epilepsia - Tập 35 Số 1 - Trang 1-11 - 1994
Ésper A. Cavalheiro1, Maria José da Silva Fernandes2, Lechosław Turski3, Maria da Graça Naffah‐Mazzacoratti2
1Disciplina de Neurologia Experimental, Escola Paulista de Medicina, São Paulo, Brazil.
2Disciplina de Neurologia Experimental, Escola Paulista de Medicina, São Paulo (Brasil)
3Dept. of Neuropsychopharmacology, Schering AG, Berlin, Germany

Tóm tắt

Summary: Rats subjected to structural brain damage induced by sustained convulsions triggered by systemic administration of pilocarpine (PILO) are a useful model for investigation of the mechanisms essential for seizure generation and spread in rodents. After PILO administration, three distinct phases are observed: (a) an acute period of 1–2 days’ duration corresponding to a pattern of repetitive limbic seizures and status epilepticus; (b) a seizurefree (silent) period characterized by a progressive return to normal EEG and behavior of 4–44 days’ duration; and (c) a period of spontaneous recurrent seizures (SRS) starting 5‐45 days after PILO administration and lasting throughout the animal's life. PILO (320–350 mgikg intraperitoneally, i.p.) was administered to rats, and the content of hippocampal monoamines and amino acids was measured in the acute, silent, and SRS periods by liquid chromatography. Norepinephrine (NE) level was decreased during all periods whereas dopamine (DA) content was increased. Serotonin (5‐hydroxytryptamine, 5‐HT) was increased only in the acute period. Utilization rate measurement of monoamines showed increased NE consumption and decreased DA consumption during all phases. 5‐HT utilization rate was increased only in the acute period. Amino acid content showed a decrease in aspartate (ASP) and glutamate (GLU) concentrations associated with increased y‐aminobutyric acid (GABA) level during the acute period. The silent phase was characterized by a decrease in glycine (GLY) and GABA levels and an increase in GLU concentration. The SRS period showed an increase in all amino acid concentrations. These findings show important neurochemical changes in the course of establishment of an epileptic focus after brain damage induced by status epilepticus triggered by pilocarpine.

Từ khóa


Tài liệu tham khảo

10.1016/0028-3908(90)90027-O

10.1111/j.1476-5381.1975.tb07378.x

10.1007/BF00973756

Babb TL, 1987, Surgical treatment of the epilepsies, 511

10.1016/0306-4522(80)90049-4

10.1016/0091-3057(86)90591-5

10.1016/0014-4886(91)90070-S

10.1016/0028-3908(79)90098-4

Campos CJ, 1986, Catecholaminergic influences on induced hippocampal paroxysmal activity in rats, Acta Physiol Pharmacol Lat Am, 36, 233

Cavalheiro EA., 1990, GAD‐Immunoreactive neurons are preserved in the hippocampus of rats with spontaneous recurrent seizures, Braz J Med Biol Res, 23, 555

10.1016/0091-3057(81)90263-X

10.1111/j.1528-1157.1991.tb05533.x

10.1016/0006-8993(90)91054-K

10.1016/0006-8993(80)90289-9

Corsellis JAN, 1976, Greenfield's neuropathology, 771

10.1016/0006-8993(82)90024-5

10.1016/0024-3205(88)90267-6

10.1016/0006-8993(85)91357-5

10.1016/0304-3940(91)90153-K

10.3758/BF03337227

10.1016/0024-3205(86)90455-8

10.1016/0006-8993(84)90956-9

10.1016/S0378-4347(00)84829-X

10.1016/0306-4522(89)90286-8

Laird HE., 1983, Abnormal concentration and turnover rates of nor‐epinephrine and dopamine in brains of genetically seizuresusceptible rats, Epilepsia, 24, 107

Laird HE, 1976, Taurine, 23

Laird HE, 1987, Neurotransmitter systems and epilepsy, 1

10.1016/0006-8993(85)91422-2

10.1016/S0149-7634(05)80076-4

10.1002/ana.410290513

10.1016/0014-4886(82)90240-0

10.1016/0006-8993(79)90967-3

Mathern GW, 1992, Onset and patterns of hippocampal sprouting in the rat kainate seizure model: evidence for progressive cell loss and neoinnervation in regio inferior and superior, Dendron, 1, 69

Mazzacoratti MGN, 1990, HPLC determination of norepinephrine, 5‐hydroxytyramine and 5‐hydroxytryptamine in rat brain using dodecyl‐sulphate as ion‐pair, Braz J Med Biol Res, 23, 255

Meldrum BS., 1991, Neurobehavioral problems in epilepsy, 35

10.1016/0014-2999(75)90284-8

Mello LEAM, 1994, Granule cell dispersion in relation to mossy fiber sprouting, hippocampal cell loss, silent period and seizure frequency in pilocarpine model of epilepsy, Epilepsia

MillanMH ChapmanA MeldrumBS.Pilocarpine induces neuronal release of glutamate and aspartate in the hippocampus [Abstract]. Third IBRO World Congress of Neurosciences Montreal 1991:221.

NajlerahimA WilliansSF PearsonRCA JefferyJGR.Increased GAD mRNA in the chronic epileptic focus induced by intrahippocampal tetanus toxin [Abstract].Focus on epilepsy symposium. Third IBRO World Congress of Neurosciences Montreal Quebec 1991:10.

10.1038/292165a0

10.1038/301520a0

10.1002/syn.890050210

10.1111/j.1471-4159.1985.tb10522.x

10.1212/WNL.30.11.1169

10.1016/0006-8993(78)90951-4

10.1111/j.1471-4159.1982.tb08708.x

10.1016/0306-4522(83)90113-6

10.1016/0920-1211(91)90096-X

10.1016/0166-4328(83)90136-5

10.1016/0006-8993(86)91247-3

10.1016/0014-4886(90)90008-G

10.1016/0361-9230(79)90043-1

10.1016/S0378-4347(00)80426-0

10.1016/0014-4886(84)90020-7

10.1016/0014-2999(87)90468-7

Thông tin
Thông tin xuất bản

Epilepsia

Tập 35 Số 1

1-11

Thông tin tác giả