Spermiogenesis in Odontophrynus cultripes (Amphibia, Anura, Leptodactylidae): Ultrastructural and cytochemical studies of proteins using E‐PTA

Journal of Morphology - Tập 207 Số 3 - Trang 303-314 - 1991
Sônia Nair Báo1, Gustavo C. Dalton1, Sérgio Ferreira de Oliveira1
1Department of Genetics and Morphology, and Department of Cell Biology, University of Brasília, 70.919, Brasília, DF., Brazil

Tóm tắt

AbstractSpermiogenesis in the South American leptodactylid frog Odontophrynus cultripes was analyzed ultrastructurally. The spermatids undergo morphological modification while still enclosed in microtubule‐rich processes of Sertoli cells. Electron‐dense plates resembling junctional structures appear in regions at which the spermatids lie in close contact with the surface of Sertoli cell processes. Spermatid differentiation can be divided into five distinct stages based mainly on chromatin condensation. In the late stages, the densely compacted chromatin loses reactivity to ethanolic phosphotungstic acid (E‐PTA). Helical arrangements of microtubules appear in the cytoplasm that surrounds the spermatid nucleus after the second stage. The acrosomal vesicle differentiates into a cone‐shaped acrosome that caps the anterior region of the nucleus. The connecting piece, located in the flagellum implantation zone, has transverse striations, and is continuous with the axial rod. The tail is formed by a 9 + 2 axoneme, an undulating membrane, and an axial rod that is rich in basic proteins as demonstrated by E‐PTA staining.

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Thông tin xuất bản

Journal of Morphology

Tập 207 Số 3

303-314

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