Spectroscopic Studies on the Interaction of Synthetic Food Colorants with Bovine Serum Albumin

Zeitschrift fur Physikalische Chemie - Tập 225 Số 4 - Trang 455-468 - 2011
Baosheng Liu, Jing Wang1, Xue Chun-li1, Chao Yang1, Yun‐Kai Lv1
1Hebei University, College of Chemistry and Environmental Science, Baoding 071002, Volksrepublik China

Tóm tắt

Abstract The conjugation between bovine serum albumin (BSA) and three synthetic food colorants like tartrazine (TTZ), sunset yellow (SY) and erythrosine (ETS) was investigated by fluorescence spectroscopy in tris-HCl buffer solution of pH 7.40. The results indicated that TTZ, SY and ETS could form non-covalent compounds with BSA, which led to the static quenching of endogenous fluorescence of BSA. The binding ability followed the pattern: ETS > TTZ > SY. The values of Hill's coefficients were slightly more than 1 in all systems. Thermodynamic parameters indicated that hydrogen bond and Van der Waals played a major role in the binding of synthetic food colorants to BSA; besides, the competitive experiments suggested that the primary binding site for three synthetic food colorants was located at site I in sub-domain II A of BSA. According to Förster's non-radiative energy transfer theory, the binding distances (r) between synthetic food colorants and BSA were much smaller than 7ߙnm. Meanwhile, synthetic food colorants reduced the binding constants between ciprofloxacin hydrochloride (CPFX) and BSA, leading to the change of medicinal efficacy.

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Tài liệu tham khảo

Chen G. Q., 2009, Spectrosc. Spect. Anal., 29, 2518

10.1016/j.aca.2006.10.002

10.1007/BF02976239

10.1016/j.chroma.2005.11.061

10.1016/j.dyepig.2009.05.003

10.1016/j.talanta.2006.02.052

10.1016/j.jhazmat.2008.07.132

10.1016/j.molstruc.2007.09.002

Sjöholm I., 1979, Mol. Pharmacol., 16, 767

10.1016/j.dyepig.2008.07.013

10.1007/s00216-003-1964-4

10.1016/j.molstruc.2009.04.027

10.1007/s10895-008-0346-x

10.1007/s11434-006-2108-y

10.1016/j.jlumin.2009.09.002

10.1016/j.jpba.2009.09.004

10.1111/j.1749-6632.1973.tb20465.x

10.1021/bi00514a017

10.1016/j.saa.2008.06.027

10.1016/j.saa.2009.05.010

10.1016/j.jphotobiol.2009.08.001

10.1016/j.molstruc.2007.09.001

10.1016/S0065-3233(08)60640-3

10.1016/j.jpba.2010.01.006

10.3329/jhpn.v27i1.3315

10.1110/ps.9.8.1455

Vorum H., 1996, J. Pharm. Pharmacol., 48, 870, 10.1111/j.2042-7158.1996.tb03990.x

10.1002/andp.19484370105

Feng X. Z., 1998, Chin. J. Anal. Chem., 26, 154

10.1021/jp012314u

10.1016/j.dyepig.2008.02.008

10.1126/science.283.5408.1676

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Thông tin xuất bản

Zeitschrift fur Physikalische Chemie

Tập 225 Số 4

455-468

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