Specific lipofuscin staining as a novel biomarker to detect replicative and stress-induced senescence. A method applicable in cryo-preserved and archival tissues

Aging - Tập 5 Số 1 - Trang 37-50 - 2012
EA Georgakopoulou1, K Tsimaratou1, Konstantinos Evangelou1, Marcos-PJ Fernandez2, Vassilios Zoumpourlis3, IP Trougakos4, Dimitris Kletsas5, Jiří Bártek6,7, Manuel Serrano2, Vassilis G. Gorgoulis8,1
1Molecular Carcinogenesis Group, Department of Histology and Embryology, School of Medicine, University of Athens, Greece
2Tumor Suppression Group, Molecular Oncology Program, Spanish National Cancer Centre (CNIO), Melchor Fernández Almagro 3, Madrid E-28029, Spain
3National Hellenic Research Foundation, Institute of Biological Research and Biotechnology, Athens, Greece
4Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Panepistimiopolis, Athens 15784, Greece
5Laboratory of Cell Proliferation and Ageing, Institute of Biology, National Centre for Scientific Research ‘Demokritos’, Athens, Greece
6Danish Cancer Society Research Center, Copenhagen, Denmark
7Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic
8Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece

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Halazonetis, 2010, Oncogene-induced senescence: the bright and dark side of the response, Curr Opin Cell Biol, 2, 816

Campisis, 1995, A biomarker that identifies senescent human cells in culture and in aging skin in vivo, Proc Natl Acad Sci U S A, 9, 9363

Narita, 2011, Impact of cellular senescence signature on ageing research, Ageing Res Rev, 1, 146

Hayflick, 1965, The limited in vitro lifespan of human diploid cell strains, Exp Cell Res, 614, 10.1016/0014-4827(65)90211-9

Serrano, 2005, Tumour biology: senescence in premalignant tumours, Nature, 43, 642

Peeper, 2005, BRAFE600-associated senescence-like cell cycle arrest of human naevi, Nature, 43, 720

Pandolfi, 2005, Crucial role of the p53-dependent cellular senescence in suppression of Pten-deficient tumorigenesis, Nature, 43, 725

Schmitt, 2005, Oncogene-induced senescence as an initial barrier in lymphoma development, Nature, 43, 660

Gorgoulis, 2006, Oncogene-induced senescence is part of the tumorigenesis barrier imposed by DNA damage checkpoints, Nature, 44, 633

Kletsas, 2005, p53-dependent ICAM-1 overexpression in senescent human cells identified in atherosclerotic lesions, Lab Invest, 8, 502

Sedivy, 2006, Cellular senescence in aging primates, Science, 31, 1257

Campisi, 2011, Four faces of cellular senescence, J Cell Biol, 19, 547

Roninson, 2004, Hallmarks of senescence in carcinogenesis and cancer therapy, Oncogene, 2, 2919

Toussaint, 2009, Protocols to detect senescence-associated beta-galactosidase (SA-betagal) activity, a biomarker of senescent cells in culture and in vivo, Nat Protoc, 4, 1798, 10.1038/nprot.2009.191

Brunk, 2004, Lipofuscin, Int J Biochem Cell Biol, 3, 1400

Grune, 2007, Lipofuscin: formation, distribution, and metabolic consequences, Ann N Y Acad Sci, 111, 97

Moussa, 1949, The sudan black B technique in cytology, J R Microsc Soc, 6, 72

Rasmussen, 1961, A method of staining the statoacoustic nerve in bulk with Sudan black B, Anat Rec, 13, 465, 10.1002/ar.1091390403

Grune, 2010, Lipofuscin: detection and quantification by microscopic techniques, Methods Mol Biol, 59, 173

Kletsas, 2011, Human lung fibroblasts prematurely senescent after exposure to ionizing radiation enhance the growth of malignant lung epithelial cells in vitro and in vivo, Int J Oncol, 3, 989

Lowe, 1997, Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a, Cell, 8, 593

Campisi, 2005, Senescent cells, tumor suppression, and organismal aging: good citizens, bad neighbors, Cell, 12, 513, 10.1016/j.cell.2005.02.003

Roninson, 2000, Effects of p21Waf1/Cip1/Sdi1 on cellular gene expression: implications for carcinogenesis, senescence, and age-related diseases, Proc Natl Acad Sci U S A, 9, 4291

Porter, 2001, Polyamine depletion in human melanoma cells leads to G1 arrest associated with induction of p21WAF1/CIP1/SDI1, changes in the expression of p21-regulated genes, and a senescence-like phenotype, Cancer Res, 6, 7754

Gorgoulis, 2009, Modulation of the E2F1-driven cancer cell fate by the DNA damage response machinery and potential novel E2F1 targets in osteosarcomas, Am J Pathol, 17, 376

Pool, 1999, Double immunolabeling of neuropeptides in the human hypothalamus as analyzed by confocal laser scanning fluorescence microscopy, J Histochem Cytochem, 4, 229

Dako: Special Stains and H & E Education Guide. 2010, Edited by George L. Kumar JAK.

Ittmann, 2003, Cellular senescence in the pathogenesis of benign prostatic hyperplasia, Prostate, 5, 30

Cristofalo, 2000, Is beta-galactosidase staining a marker of senescence in vitro and in vivo?, Exp Cell Res, 25, 162

Hu, 2005, The limitations and validities of senescence associated-beta-galactosidase activity as an aging marker for human foreskin fibroblast Hs68 cells, Exp Gerontol, 4, 813

Hwang, 2006, Senescence-associated β -galactosidase is lysosomal β-galactosidase, Aging cell, 5, 187, 10.1111/j.1474-9726.2006.00199.x

Terman, 2002, The mitochondrial-lysosomal axis theory of aging: accumulation of damaged mitochondria as a result of imperfect autophagocytosis, Eur J Biochem, 26, 1996

Mildvan, 1959, Rate and magnitude of age pigment accumulation in the human myocardium, J Gerontol, 1, 430

Korth, 2012, Human and rat brain lipofuscin proteome, Proteomics, 1, 2445

Blagosklonny, 2012, Tumor suppression by p53 without apoptosis and senescence: conundrum or rapalog-like gerosuppression?, Aging, 4, 450, 10.18632/aging.100475

Harris, 1981, Quantitative studies of the autofluorescence derived from neuronal lipofuscin, J Microsc, 12, 249

Charles C: “Pigments and Minerals”, Theory and Practice of Histological Techniques Edited by Bancroft JD GM. Churchill Livingstone, 2002, p. 208.

do Carmo, 2007, An improved and cost-effective methodology for the reduction of autofluorescence in direct immunofluorescence studies on formalin-fixed paraffin-embedded tissues, Eur J Histochem, 5, 59

Sedivy, 2008, Cellular senescence and organismal aging, Mech Ageing Dev, 12, 467

Serrano, 2006, The power and the promise of oncogene-induced senescence markers, Nat Rev Cancer, 6, 472, 10.1038/nrc1884

Keith, 2012, Cancer cell senescence: a new frontier in drug development, Drug Discov Today, 1, 269

Schmitt, 2010, Cellular senescence predicts treatment outcome in metastasised colorectal cancer, Br J Cancer, 10, 505

Halazonetis, 2005, Activation of the DNA damage checkpoint and genomic instability in human precancerous lesions, Nature, 43, 907