Spatial variations in intestinal skatole production and microbial composition in broilers

Animal Science Journal - Tập 90 Số 3 - Trang 412-422 - 2019
Guiqin Yang1, Pei Zhang1, Haiying Liu1, Xin Zhu1, Weiguo Dong1
1College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, P. R. China

Tóm tắt

AbstractSpatial variations in intestinal skatole production and microbial composition in broilers were evaluated. Fifteen 42‐day‐old broilers were slaughtered. Samples were taken from the broilers’ ileum, cecum, and rectum and analyzed for skatole levels. Denaturing gradient gel electrophoresis (DGGE) technique was used to analyze the microbial community from the intestinal digesta. The skatole levels could be arranged in decreasing order: cecum > rectum > ileum. Cecal lactate and acetate levels were higher than those of ileum and rectum (p < 0.01). Cecal microbial diversity and richness were higher than those of ileum (p < 0.05). One specific DGGE band was found in cecal sample and is closely related to Bacteroides uniformis. Cecum and rectum samples consisted of three coexistence bands, the related bacteria included Lactobacillus vaginalis and two members of Candidatus Arthromitus. The total bacterial population in cecum was higher than that in ileum and rectum (p < 0.05). Skatole levels were positively correlated with microbial Shannon‐Wiener index, richness, total bacteria (p < 0.01) and Lactobacilli and Bifidobacterium (p < 0.05) populations. These results suggest that the variations in fermentation patterns are more likely to explain differences in intestinal skatole level. Bacteroides uniformis may play a role in the production of skatole.

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Animal Science Journal

Tập 90 Số 3

412-422

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